2023
High dietary K+ intake inhibits proximal tubule transport
Wang T, Liu T, Xu S, Frindt G, Weinstein A, Palmer L. High dietary K+ intake inhibits proximal tubule transport. American Journal Of Physiology. Renal Physiology 2023, 325: f224-f234. PMID: 37318989, PMCID: PMC10396284, DOI: 10.1152/ajprenal.00013.2023.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFree-flow micropunctureUrine volumeFractional excretion of Na<sup>+</sup>Proximal tubulesFiltration rateFractional NaNephron segmentsExcretion of Na<sup>+</sup>Dietary K<sup>+</sup>NHE3 protein expressionExchanger isoform 3Increased urine volumeIncreased fractional excretionVolume reabsorptionAbsolute NaKidney functionProtein expressionIsoform 3PT transportAnesthetized animalsReabsorptionWestern blottingRatsExcretion
2021
Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice
Xu S, Li J, Yang L, Wang CJ, Liu T, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice. Pflügers Archiv - European Journal Of Physiology 2021, 473: 1749-1760. PMID: 34455480, PMCID: PMC8528772, DOI: 10.1007/s00424-021-02611-5.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFemale miceUrine volumeNaCl cotransporterLow K intakeMeasurement of functionSex differencesNatriuretic responseFractional excretionK intakeTransport protein expressionFiltration rateNCC abundanceNCC expressionRenal clearanceDistal nephronLK dietCation excretionPlasma KNCC proteinNKCC2 expressionWestern blottingProtein expressionMiceFENa
2010
Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells
Duan Y, Weinstein A, Weinbaum S, Wang T. Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 21860-21865. PMID: 21106755, PMCID: PMC3003103, DOI: 10.1073/pnas.1015751107.Peer-Reviewed Original ResearchConceptsProximal tubule cellsMouse proximal tubuleNa/K-ATPaseTubule cellsApical NHE3Proximal tubulesMouse proximal tubule cellsFSS-induced changesApical plasma membraneV-ATPaseAmount of protein expressionBasolateral membraneWestern blot analysisNHE3Volume reabsorptionTransporter expressionBasolateral transportProtein expressionMPT cellsShear stress-induced changesVacuolar H(+)-ATPaseBlock microtubule polymerizationControl cellsModulate cellular functionsTransporter localization
2005
Role of PDZK1 in membrane expression of renal brush border ion exchangers
Thomson RB, Wang T, Thomson BR, Tarrats L, Girardi A, Mentone S, Soleimani M, Kocher O, Aronson PS. Role of PDZK1 in membrane expression of renal brush border ion exchangers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13331-13336. PMID: 16141316, PMCID: PMC1201624, DOI: 10.1073/pnas.0506578102.Peer-Reviewed Original ResearchConceptsProximal tubulesExpression of NHE3Functional activityRole of PDZK1Protein PDZK1Reabsorption of NaBrush border expressionMammalian kidneyCFEXBrush border localizationMutant miceGST fusion proteinProtein expressionKidneyBrush border membrane proteinsExchanger NHE3Membrane expressionNHE3Brush borderBrush border membrane vesiclesPDZK1 interactionTubulesNormal expressionMembrane proteinsPDZK1