2012
Regulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport
Du Z, Yan Q, Wan L, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f386-f395. PMID: 22552936, PMCID: PMC3433864, DOI: 10.1152/ajprenal.00531.2011.Peer-Reviewed Original ResearchMeSH Keywords8-Bromo Cyclic Adenosine MonophosphateAlgorithmsAnimalsBenzazepinesBicarbonatesBiological Transport, ActiveChloridesCyclic AMP-Dependent Protein KinasesDopamineDopamine AntagonistsDopamine D2 Receptor AntagonistsFemaleIsoquinolinesKidney GlomerulusKidney TubulesKidney Tubules, ProximalMacrolidesMiceMicrovilliProton-Translocating ATPasesReceptors, Dopamine D1SodiumSulfonamidesSulpirideConceptsNa/H exchanger 3Impact of dopamineNa(+) transportNa-K-ATPase activityResponse to volume expansionProximal tubules in vitroCl(-) reabsorptionPKA-mediated mechanismMicroperfused in vitroEffects of exogenous dopamineMouse proximal tubuleNa-K-ATPaseProximal tubule reabsorptionStimulation of Cl(-PKA inhibitor H89Luminal flowH-ATPase activityTubules in vitroLuminal flow rateNHE3 activityDA1 receptorsNa/H exchangeTubule reabsorptionProximal tubulesInhibitor H89
2011
Chloride Channel (Clc)-5 Is Necessary for Exocytic Trafficking of Na+/H+ Exchanger 3 (NHE3)*
Lin Z, Jin S, Duan X, Wang T, Martini S, Hulamm P, Cha B, Hubbard A, Donowitz M, Guggino SE. Chloride Channel (Clc)-5 Is Necessary for Exocytic Trafficking of Na+/H+ Exchanger 3 (NHE3)*. Journal Of Biological Chemistry 2011, 286: 22833-22845. PMID: 21561868, PMCID: PMC3123051, DOI: 10.1074/jbc.m111.224998.Peer-Reviewed Original ResearchConceptsKO miceTrafficking of NHE3Proximal tubulesOpossum kidney cellsNHE3 activityDent's diseaseClC-5Surface expressionNHE3 surface expressionKidney cellsRenal proximal tubulesTotal protein levelsChloride/proton exchangerRates of basalReduced surface expressionKnockdown cellsParathyroid hormoneWT miceDegree of inhibitionCLCN5 geneSurface NHE3MiceTubule perfusionReduced expressionTwo-photon microscopy
2007
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity
Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. American Journal Of Physiology. Renal Physiology 2007, 293: f212-f218. PMID: 17409282, DOI: 10.1152/ajprenal.00042.2007.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAnimalsCells, CulturedColforsinCyclic AMP-Dependent Protein KinasesElectrophoresis, Polyacrylamide GelKidneyKidney Tubules, ProximalMaleMicrovilliParathyroid HormonePhosphodiesterase InhibitorsPhosphorylationRatsRats, Sprague-DawleySerineSodiumSodium RadioisotopesSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersStimulation, ChemicalConceptsSerine 552NHE3 phosphorylationNHE3 activityMicrovillar membrane vesiclesPhosphorylation of NHE3Direct phosphorylationPhosphospecific antibodiesSprague-Dawley ratsExchanger type 3PKA activationMembrane vesiclesSite-specific changesPhosphorylationTransport activityOpossum kidney cellsParathyroid hormoneIntravenous infusionRat modelExchange activityKidney cellsOKP cellsNHE3 inhibitionPKACell modelExact role
2006
Flow-activated transport events along the nephron
Wang T. Flow-activated transport events along the nephron. Current Opinion In Nephrology & Hypertension 2006, 15: 530-536. PMID: 16914967, DOI: 10.1097/01.mnh.0000242180.46362.c4.Peer-Reviewed Original ResearchConceptsBrush-border microvilliProximal tubulesCultured proximal tubule cellsProximal tubules in vitroGlomerular filtration rateProximal tubule cellsBrush-borderH-ATPase activityTubules in vitroNHE3 activityHCO3- absorptionENOS translocationTubule cellsFiltration rateMg2+ transportReceptor localizationSignal transduction mechanismsH-ATPaseHormonal regulationCa2+NephronTubulesResponse to flowTransduction mechanismsIntact actin cytoskeleton