2016
Flow-activated proximal tubule function underlies glomerulotubular balance.
Du Z, Duan Y, Yan Q, Weinbaum S, Weinstein A, Wang T. Flow-activated proximal tubule function underlies glomerulotubular balance. 2016, 46: 105-117. PMID: 31105444, PMCID: PMC6519745.Peer-Reviewed Original ResearchProximal tubulesModulating Na<sup>+</sup>Isolated perfused proximal tubulesTubule cell volumeExchanger isoform 3Proximal tubule cellsProximal tubule functionPerfused proximal tubulesH-ATPase activityMouse tubulesNHE3 expressionStimulate sodiumApical membraneTubule functionBicarbonate absorptionTubule cellsReceptor-mediatedGlomerulotubular balanceMouse kidneyIsoform 3H-ATPaseCellular mechanismsTubulesTransporter densityNHE3
2013
Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion
Li H, Du Z, Barone S, Rubera I, McDonough A, Tauc M, Zahedi K, Wang T, Soleimani M. Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion. Journal Of Molecular Medicine 2013, 91: 951-963. PMID: 23508938, PMCID: PMC3730089, DOI: 10.1007/s00109-013-1015-3.Peer-Reviewed Original ResearchConceptsNa+/H+ exchanger NHE3Proximal convoluted tubulesKO miceMetabolic acidosisBicarbonate reabsorptionDays of acid loadingIsolated proximal convoluted tubulesConvoluted tubulesIn vitro microperfusion studyCompared to WT miceNHE3 knockout miceProximal tubule NHE3Acid loadNH4Cl acid loadMild metabolic acidosisWT miceNHE3Microperfusion studiesKnockout miceBicarbonate absorptionMutant miceVolume reabsorptionFloxed miceNH4Cl loadingTransgenic mice
2011
DOPAMINE MODULATES FLOW‐DEPENDENT TRANSPORT BY NHE3, BUT NOT H+‐ATPASE, IN MOUSE PROXIMAL TUBULES
Du Z, Gotoh N, Weinbaum S, Weinstein A, Wang T. DOPAMINE MODULATES FLOW‐DEPENDENT TRANSPORT BY NHE3, BUT NOT H+‐ATPASE, IN MOUSE PROXIMAL TUBULES. The FASEB Journal 2011, 25: 1041.18-1041.18. DOI: 10.1096/fasebj.25.1_supplement.1041.18.Peer-Reviewed Original ResearchEffects of DAProtein kinase A inhibitor H89Proximal tubules in vitroPKA-mediated mechanismProximal tubule transportReabsorption of fluidTubules in vitroHCO3- absorptionLuminal perfusion rateTubule transportJHCO3Cl- transportProximal tubulesNHE3Perfusion rateDopamineSCH23390JNaPresence of DAInhibitory effectSignaling mechanismsH+-ATPaseDA1No effectFlow-dependent transport
2010
Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells
Duan Y, Weinstein A, Weinbaum S, Wang T. Shear stress-induced changes of membrane transporter localization and expression in mouse proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 21860-21865. PMID: 21106755, PMCID: PMC3003103, DOI: 10.1073/pnas.1015751107.Peer-Reviewed Original ResearchConceptsProximal tubule cellsMouse proximal tubuleNa/K-ATPaseTubule cellsApical NHE3Proximal tubulesMouse proximal tubule cellsFSS-induced changesApical plasma membraneV-ATPaseAmount of protein expressionBasolateral membraneWestern blot analysisNHE3Volume reabsorptionTransporter expressionBasolateral transportProtein expressionMPT cellsShear stress-induced changesVacuolar H(+)-ATPaseBlock microtubule polymerizationControl cellsModulate cellular functionsTransporter localizationROLE OF DOPAMINE IN MODULATING FLOW‐DEPENDENT SODIUM AND BICARBONATE TRANSPORT IN MOUSE PROXIMAL TUBULE
Du Z, Yan Q, Weinbaum S, Weinstein A, Wang T. ROLE OF DOPAMINE IN MODULATING FLOW‐DEPENDENT SODIUM AND BICARBONATE TRANSPORT IN MOUSE PROXIMAL TUBULE. The FASEB Journal 2010, 24: 1024.2-1024.2. DOI: 10.1096/fasebj.24.1_supplement.1024.2.Peer-Reviewed Original ResearchProximal tubule transportEffects of dopamineMouse proximal tubuleTubule transportProximal tubulesNHE3 endocytosisCAMP-dependent protein kinase inhibitor H89PKA-mediated mechanismMicroperfused in vitroProtein kinase inhibitor H89Kidney proximal tubulesReabsorption of fluidLuminal flow rateKinase inhibitor H89Sodium reabsorptionApical membraneHCO-3 absorptionInhibitor H89DopaminePerfusion rateTubulesSCH23390NHE3Net reabsorptionSulpiride
2005
Axial flow modulates proximal tubule NHE3 and H-ATPase activities by changing microvillus bending moments
Du Z, Yan Q, Duan Y, Weinbaum S, Weinstein A, Wang T. Axial flow modulates proximal tubule NHE3 and H-ATPase activities by changing microvillus bending moments. American Journal Of Physiology. Renal Physiology 2005, 290: f289-f296. PMID: 16144961, DOI: 10.1152/ajprenal.00255.2005.Peer-Reviewed Original ResearchConceptsProximal tubules in vitroHCO3- absorptionTubules in vitroBrush-border microvilliHCO3- permeabilityH(+)-ATPase inhibitor bafilomycinInhibition of NHE3Proximal tubule NHE3Transepithelial potential differenceH-ATPase activityLuminal cell membraneH(+)-ATPaseTransepithelial PDNa+ absorptionParacellular permeabilityH(+)-ATPase activityInhibitor EIPAMembrane transport activityProximal tubulesHCO3- reabsorptionNHE3HCO3- transportH-ATPaseBicarbonate transportPermeability changesRole of PDZK1 in membrane expression of renal brush border ion exchangers
Thomson RB, Wang T, Thomson BR, Tarrats L, Girardi A, Mentone S, Soleimani M, Kocher O, Aronson PS. Role of PDZK1 in membrane expression of renal brush border ion exchangers. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 13331-13336. PMID: 16141316, PMCID: PMC1201624, DOI: 10.1073/pnas.0506578102.Peer-Reviewed Original ResearchConceptsProximal tubulesExpression of NHE3Functional activityRole of PDZK1Protein PDZK1Reabsorption of NaBrush border expressionMammalian kidneyCFEXBrush border localizationMutant miceGST fusion proteinProtein expressionKidneyBrush border membrane proteinsExchanger NHE3Membrane expressionNHE3Brush borderBrush border membrane vesiclesPDZK1 interactionTubulesNormal expressionMembrane proteinsPDZK1
2001
Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron
Wang T, Hropot M, Aronson P, Giebisch G. Role of NHE isoforms in mediating bicarbonate reabsorption along the nephron. American Journal Of Physiology. Renal Physiology 2001, 281: f1117-f1122. PMID: 11704563, DOI: 10.1152/ajprenal.2001.281.6.f1117.Peer-Reviewed Original ResearchConceptsLoop of HenleHOE 694Proximal tubulesAddition of ethylisopropylamilorideDistal convoluted tubuleBicarbonate reabsorptionConvoluted tubulesSitu microperfusionRat kidneySignificant inhibitionNHE isoformsHenlePredominant isoformTubulesNHE3Functional roleInhibitionNHE2Apical membraneIsoformsApical isoformKidneyMicroperfusionEthylisopropylamilorideNephron
1998
Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger
Schultheis P, Clarke L, Meneton P, Miller M, Soleimani M, Gawenis L, Riddle T, Duffy J, Doetschman T, Wang T, Giebisch G, Aronson P, Lorenz J, Shull G. Renal and intestinal absorptive defects in mice lacking the NHE3 Na+/H+ exchanger. Nature Genetics 1998, 19: 282-285. PMID: 9662405, DOI: 10.1038/969.Peer-Reviewed Original ResearchConceptsAcid-base balanceAbsorptive defectsNHE3-deficient miceFluid volume homeostasisRenal proximalSlight diarrheaPlasma aldosteroneBlood pressureHomozygous mutant miceExchanger mRNANHE2 isoformsBlood analysisMutant miceCompensatory mechanismsFluid absorptionNHE3 functionVolume homeostasisMiceDistribution of NHE3Cl-/HCO3Channel activityNHE3KidneyATPase mRNAIntestine