2021
Restoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease
Du Z, Tian X, Ma M, Somlo S, Weinstein AM, Wang T. Restoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease. JCI Insight 2021, 6: e146041. PMID: 33886508, PMCID: PMC8262298, DOI: 10.1172/jci.insight.146041.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateGlomerulotubular balanceRenal cyst formationCyst formationReceptor 1 antagonistPolycystic kidney diseaseKidney weightUntreated miceDA1 antagonistControl miceKidney diseaseFiltration rateFractional reabsorptionCystic indexMouse modelCyst growthConditional KOHCO3- absorptionHeterozygous miceSame antagonistsMicePT transportAntagonistEpithelial ciliaHCO3- transport
2017
Regulation of glomerulotubular balance: flow-activated proximal tubule function
Wang T, Weinbaum S, Weinstein A. Regulation of glomerulotubular balance: flow-activated proximal tubule function. Pflügers Archiv - European Journal Of Physiology 2017, 469: 643-654. PMID: 28271233, PMCID: PMC6162058, DOI: 10.1007/s00424-017-1960-8.Peer-Reviewed Original ResearchConceptsNa/H exchanger isoform 3Proximal tubulesTubule functionIntracellular Ca2+ signalingProximal tubule transportMammalian proximal tubuleAcid-base transportersProximal tubule functionRegulation of sodiumCa2+ signalingHCO3- absorptionStimulate Na+Tubule transportMicropuncture studiesSolute reabsorptionPeritubular membraneBrush border membraneBrush border microvilliGlomerulotubular balanceHCO3- transportIsoform 3Flow velocity changesTubulesRegulatory mechanismsTransporter availability
2016
Flow-activated proximal tubule function underlies glomerulotubular balance.
Du Z, Duan Y, Yan Q, Weinbaum S, Weinstein A, Wang T. Flow-activated proximal tubule function underlies glomerulotubular balance. 2016, 46: 105-117. PMID: 31105444, PMCID: PMC6519745.Peer-Reviewed Original ResearchProximal tubulesModulating Na<sup>+</sup>Isolated perfused proximal tubulesTubule cell volumeExchanger isoform 3Proximal tubule cellsProximal tubule functionPerfused proximal tubulesH-ATPase activityMouse tubulesNHE3 expressionStimulate sodiumApical membraneTubule functionBicarbonate absorptionTubule cellsReceptor-mediatedGlomerulotubular balanceMouse kidneyIsoform 3H-ATPaseCellular mechanismsTubulesTransporter densityNHE3
2015
Regulation of glomerulotubular balance. III. Implication of cytosolic calcium in flow-dependent proximal tubule transport
Du Z, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. III. Implication of cytosolic calcium in flow-dependent proximal tubule transport. American Journal Of Physiology. Renal Physiology 2015, 308: f839-f847. PMID: 25651568, PMCID: PMC4398834, DOI: 10.1152/ajprenal.00601.2014.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBicarbonatesBiological TransportCalciumCalcium-Transporting ATPasesChelating AgentsCytosolEnzyme InhibitorsIn Vitro TechniquesInositol 1,4,5-Trisphosphate ReceptorsKidney Tubules, ProximalKineticsMice, Inbred C57BLPerfusionRenal ReabsorptionSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsNa/H exchanger 3Luminal Ca(2Flow-induced increaseCytosolic calciumProximal tubulesCa(2+) chelator BAPTA-AMAntagonist 2-aminoethoxydiphenyl borateCa-ATPase inhibitor thapsigarginProximal tubule transportNa(+) reabsorptionMicroperfused in vitroMouse proximal tubuleChelator BAPTA-AMH-ATPase activityNo effectControl tubulesInhibitor thapsigarginBAPTA-AMIntracellular Ca(2Decreased transportTubule transportBaseline transportStimulates Na(+Modulate Na(+Glomerulotubular balance
2010
Mechanotransduction in the renal tubule
Weinbaum S, Duan Y, Satlin L, Wang T, Weinstein A. Mechanotransduction in the renal tubule. American Journal Of Physiology. Renal Physiology 2010, 299: f1220-f1236. PMID: 20810611, PMCID: PMC3006307, DOI: 10.1152/ajprenal.00453.2010.Peer-Reviewed Original ResearchConceptsCortical collecting ductCa(2+) signalingProximal tubulesModulation of Na(+Stretch-activated ion channelsDistal nephronRenal epitheliumWater reabsorptionRenal tubulesGlomerulotubular balanceInner earEndothelial cellsIon channelsHair cellsIntracellular signalingBone cellsCa(2TubulesPrimary ciliaIntracellular cytoskeletonCellsShear stressHydrodynamic forcesApical structuresFluid shear stress
2007
Flow-dependent transport in a mathematical model of rat proximal tubule
Weinstein A, Weinbaum S, Duan Y, Du Z, Yan Q, Wang T. Flow-dependent transport in a mathematical model of rat proximal tubule. American Journal Of Physiology. Renal Physiology 2007, 292: f1164-f1181. PMID: 17213461, DOI: 10.1152/ajprenal.00392.2006.Peer-Reviewed Original ResearchConceptsNa(+) reabsorptionRat proximal tubulesProximal tubulesModel tubuleDiuretic effectMouse proximal tubuleLuminal perfusion rateLow flowsSolute transportNaCl reabsorptionPeritubular transportTranscellular NaCl reabsorptionCarbonic anhydrase inhibitionGlomerulotubular balanceTubulesReabsorptionTransporter densityLuminal flowModel calculationsRatsFlow-dependent transportTransporter insertionMathematical modelPerfusion rateRate of hydrationFlow‐dependent transport in a mathematical model of rat proximal tubule
Weinstein A, Weinbaum S, Duan Y, Du Z, Yan Q, Wang T. Flow‐dependent transport in a mathematical model of rat proximal tubule. The FASEB Journal 2007, 21: a827-a828. DOI: 10.1096/fasebj.21.6.a827-d.Peer-Reviewed Original ResearchRat proximal tubulesNa+ reabsorptionProximal tubulesDiuretic effectModel tubuleOsmotic diuretic effectLow flowsSolute transportMouse tubulesLuminal perfusion rateNaCl reabsorptionPeritubular transportTranscellular NaCl reabsorptionCarbonic anhydrase inhibitionGlomerulotubular balanceTubulesReabsorptionTransporter densityLuminal flowMathematical modelRate of hydrationFlow-dependent transportInlet flowTransportTorque calculation