A novel genetic strategy reveals unexpected roles of the Swi–Snf–like chromatin-remodeling BAF complex in thymocyte development
Jani A, Wan M, Zhang J, Cui K, Wu J, Preston-Hurlburt P, Khatri R, Zhao K, Chi T. A novel genetic strategy reveals unexpected roles of the Swi–Snf–like chromatin-remodeling BAF complex in thymocyte development. Journal Of Experimental Medicine 2008, 205: 2813-2825. PMID: 18955569, PMCID: PMC2585832, DOI: 10.1084/jem.20080938.Peer-Reviewed Original ResearchConceptsPoint mutantsUnexpected roleImportant gene functionsThymocyte developmentNovel genetic strategyPoint mutationsEarly thymocyte developmentMammalian geneticsChromatin templatesSWI-SNFBAF complexGene functionATPase subunitsDeletion mutantsFactor complexCD4 locusTarget genesGenetic strategiesCD4 activationMutantsNovel activityPhysical interactionDeletionBRGMutationsNucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development
Yu M, Wan M, Zhang J, Wu J, Khatri R, Chi T. Nucleoprotein structure of the CD4 locus: Implications for the mechanisms underlying CD4 regulation during T cell development. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 3873-3878. PMID: 18322012, PMCID: PMC2268827, DOI: 10.1073/pnas.0800810105.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD4 AntigensChromatin ImmunoprecipitationEnhancer Elements, GeneticGene DeletionGene Expression RegulationHistonesLymphocyte SubsetsMiceMice, Inbred C57BLModels, BiologicalNucleoproteinsPromoter Regions, GeneticProtein BindingSilencer Elements, TranscriptionalT-LymphocytesTranscription FactorsConceptsT cell developmentCell developmentNucleoprotein structuresCD4 locusHeterochromatin-like structureOpen chromatin configurationStage-specific mannerSame regulatory elementsDP cellsRepression patternsTranscription activatorCD4 silencerP300 recruitmentChromatin configurationRegulatory elementsDN cellsMolecular basisMolecular mechanismsCD4 enhancerCD4 transcriptionMature T cellsCD4 regulationCD4 geneCD4 promoterSilencer