2023
LC3 conjugation to lipid droplets
Omrane M, Melia T, Thiam A. LC3 conjugation to lipid droplets. Autophagy 2023, 19: 3251-3253. PMID: 37599471, PMCID: PMC10621252, DOI: 10.1080/15548627.2023.2249390.Peer-Reviewed Original ResearchConceptsLipid dropletsResponse to different signalsDegradation of lipid dropletsUbiquitin-conjugating enzymeLong-term-starved cellsLC3-interacting regionArtificial lipid dropletsPatatin-like phospholipase domainMicrotubule-associated protein 1 light chain 3 betaFYVE domainTethering factorsE2 enzymesLIR motifLD surfaceZinc fingerEndoplasmic reticulumLipidated LC3BPerilipin 1Phospholipase domainAutophagosome formationAssembly platformPromote degradationProlonged starvationSequestosome 1ZFYVE1/DFCP1
2018
Delipidation of mammalian Atg8-family proteins by each of the four ATG4 proteases
Kauffman KJ, Yu S, Jin J, Mugo B, Nguyen N, O'Brien A, Nag S, Lystad AH, Melia TJ. Delipidation of mammalian Atg8-family proteins by each of the four ATG4 proteases. Autophagy 2018, 14: 992-1010. PMID: 29458288, PMCID: PMC6103404, DOI: 10.1080/15548627.2018.1437341.Peer-Reviewed Original ResearchConceptsAtg8-family proteinsAtg4 proteasesATG8 proteinsMammalian Atg8 proteinsMacroautophagy/autophagySimilar enzymatic activityProteolytic processing eventsLIR motifATG4 familyProcessing eventsPhysical anchoringProteinEnzymatic activityProteaseSoluble substratesATG4BTerminal glycinePhagophoreHomologLipidsDelipidationAutophagosomesAutophagyMotifSubstrate