1996
Rabies virus binding to the nicotinic acetylcholine receptor α subunit demonstrated by virus overlay protein binding assay
Gastka M, Horvath J, Lentz T. Rabies virus binding to the nicotinic acetylcholine receptor α subunit demonstrated by virus overlay protein binding assay. Journal Of General Virology 1996, 77: 2437-2440. PMID: 8887475, DOI: 10.1099/0022-1317-77-10-2437.Peer-Reviewed Original ResearchConceptsAlpha subunitNicotinic acetylcholine receptor α subunitAcetylcholine receptor α subunitElectric organ membranesVirus overlay proteinVirus overlay protein binding assaysTransfer of proteinsReceptor α subunitProtein binding assaysAChR alpha subunitOverlay proteinRabies virusΑ-subunitSubunitsGel electrophoresisAcetylcholine receptorsBinding assaysCuraremimetic neurotoxinsProteinMembraneVirusBindingAChRBlot
1993
Effects of mutations of Torpedo acetylcholine receptor alpha 1 subunit residues 184-200 on alpha-bungarotoxin binding in a recombinant fusion protein.
Chaturvedi V, Donnelly-Roberts D, Lentz T. Effects of mutations of Torpedo acetylcholine receptor alpha 1 subunit residues 184-200 on alpha-bungarotoxin binding in a recombinant fusion protein. Biochemistry 1993, 32: 9570-6. PMID: 8373764, DOI: 10.1021/bi00088a008.Peer-Reviewed Original ResearchConceptsAlpha 1 subunitFusion proteinAlpha-bungarotoxin bindingFusion protein containingOligonucleotide-directed mutagenesisEffects of mutationsPossible structure-function relationshipsStructure-function relationshipsTyr-189Dissimilar residuesPro-194Recombinant fusion proteinHigher apparent affinityAlpha subunitTyr-198Asp-195Cys-192Cys-193Protein containingFunctional interactionAmino acidsSubunitsResiduesMutationsPosition 184
1988
Chapter 9 Synthetic Peptides in the Study of the Nicotinic Acetylcholine Receptor
Hawrot E, Colson K, Lentz T, Wilson P. Chapter 9 Synthetic Peptides in the Study of the Nicotinic Acetylcholine Receptor. Current Topics In Membranes 1988, 33: 165-195. DOI: 10.1016/s0070-2161(08)60899-0.Peer-Reviewed Original Research
1985
Determination of the primary amino acid sequence specifying the alpha-bungarotoxin binding site on the alpha subunit of the acetylcholine receptor from Torpedo californica.
Wilson P, Lentz T, Hawrot E. Determination of the primary amino acid sequence specifying the alpha-bungarotoxin binding site on the alpha subunit of the acetylcholine receptor from Torpedo californica. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 8790-8794. PMID: 3866252, PMCID: PMC391523, DOI: 10.1073/pnas.82.24.8790.Peer-Reviewed Original ResearchConceptsBungarotoxin-binding sitesPrimary amino acid sequenceAmino acid sequenceAlpha subunitAmino acidsAcid sequenceAcetylcholine receptorsNicotinic acetylcholine receptorsAlpha-bungarotoxin binding sitesEndoglycosidase H.Residues 173Torpedo californicaV8 proteaseSubunitsOligosaccharide chainsProteolytic fragmentsBinding sitesPresumed siteSize of fragmentsFragmentsSynthetic peptidesSequenceBind 125IDigestionMajor determinant
1983
Binding of alpha-bungarotoxin to isolated alpha subunit of the acetylcholine receptor of Torpedo californica: quantitative analysis with protein blots.
Gershoni J, Hawrot E, Lentz T. Binding of alpha-bungarotoxin to isolated alpha subunit of the acetylcholine receptor of Torpedo californica: quantitative analysis with protein blots. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 4973-4977. PMID: 6576369, PMCID: PMC384170, DOI: 10.1073/pnas.80.16.4973.Peer-Reviewed Original ResearchConceptsAlpha subunitProtein blotsEndoglycosidase HHigh-mannose oligosaccharide chainsAcetylcholine receptorsAssociation of toxinAcetylcholine receptor subunitsReceptor-containing membranesOligosaccharide sideTorpedo electric organSingle labeled bandDirect bindingTorpedo acetylcholine receptorTorpedo californicaSubunits