Determination of the primary amino acid sequence specifying the alpha-bungarotoxin binding site on the alpha subunit of the acetylcholine receptor from Torpedo californica.
Wilson P, Lentz T, Hawrot E. Determination of the primary amino acid sequence specifying the alpha-bungarotoxin binding site on the alpha subunit of the acetylcholine receptor from Torpedo californica. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 8790-8794. PMID: 3866252, PMCID: PMC391523, DOI: 10.1073/pnas.82.24.8790.Peer-Reviewed Original ResearchConceptsBungarotoxin-binding sitesPrimary amino acid sequenceAmino acid sequenceAlpha subunitAmino acidsAcid sequenceAcetylcholine receptorsNicotinic acetylcholine receptorsAlpha-bungarotoxin binding sitesEndoglycosidase H.Residues 173Torpedo californicaV8 proteaseSubunitsOligosaccharide chainsProteolytic fragmentsBinding sitesPresumed siteSize of fragmentsFragmentsSynthetic peptidesSequenceBind 125IDigestionMajor determinant