2024
Targeting hypoxia and thrombospondin‐2 in diabetic wound healing
Huang Y, Xing H, Naud S, Kyriakides T. Targeting hypoxia and thrombospondin‐2 in diabetic wound healing. The FASEB Journal 2024, 38: e70091. PMID: 39383062, PMCID: PMC11486302, DOI: 10.1096/fj.202302429rrr.Peer-Reviewed Original ResearchConceptsThrombospondin-2Diabetic miceWound healingHIF-1aMatricellular protein thrombospondin-2Diabetic woundsImpaired wound healingWounds of diabetic miceDimethyloxalylglycine treatmentTargeting hypoxiaSustained hypoxiaDiabetic patientsTSP2 expressionCell dysfunctionIncreased neovascularizationDiabetic wound healingGenetic ablationDiabetic fibroblastsElevated glucoseReduced hypoxiaImprove healingImmunofluorescence analysisHIF-1a activationHypoxiaWestern blotting
2022
Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction
Xing H, Huang Y, Kunkemoeller B, Dahl P, Muraleetharan O, Malvankar N, Murrell M, Kyriakides T. Dysregulation of TSP2-Rac1-WAVE2 axis in diabetic cells leads to cytoskeletal disorganization, increased cell stiffness, and dysfunction. Scientific Reports 2022, 12: 22474. PMID: 36577792, PMCID: PMC9797577, DOI: 10.1038/s41598-022-26337-1.Peer-Reviewed Original ResearchConceptsCell-derived matricesCell stiffnessFamily verprolin-homologous protein 2Active Rac1Thrombospondin-2Homologous protein 2Less traction forceCytoskeleton organizationExtracellular matrix productionMajor cell populationF-actinCytoskeletal disorganizationRegulatory roleProtein 2Matrix productionCritical functionsECM productionArt microscopy techniquesNew functionsCell populationsSpindle-like shapeRac1Normal fibroblastsFibroblastsWound space
2019
Elevated Thrombospondin 2 Contributes to Delayed Wound Healing in Diabetes
Kunkemoeller B, Bancroft T, Xing H, Morris AH, Luciano AK, Wu J, Fernandez-Hernando C, Kyriakides TR. Elevated Thrombospondin 2 Contributes to Delayed Wound Healing in Diabetes. Diabetes 2019, 68: 2016-2023. PMID: 31391172, PMCID: PMC6754242, DOI: 10.2337/db18-1001.Peer-Reviewed Original ResearchConceptsThrombospondin-2TSP2 expressionDiabetic control miceWound healingEffects of hyperglycemiaImpaired wound healingUnderlying pathological mechanismsDelayed Wound HealingMajor cellular sourceBlood vessel maturationGranulation tissue formationMajor complicationsDiabetic miceControl miceTreatment strategiesDiabetesPathological mechanismsDiabetic woundsAccelerated reepithelializationCellular sourceHigh glucoseHyperglycemiaMatricellular proteinExpression contributesHexosamine pathway
2018
Tunable Hydrogels Derived from Genetically Engineered Extracellular Matrix Accelerate Diabetic Wound Healing
Morris AH, Lee H, Xing H, Stamer DK, Tan M, Kyriakides TR. Tunable Hydrogels Derived from Genetically Engineered Extracellular Matrix Accelerate Diabetic Wound Healing. ACS Applied Materials & Interfaces 2018, 10: 41892-41901. PMID: 30424595, PMCID: PMC9996546, DOI: 10.1021/acsami.8b08920.Peer-Reviewed Original ResearchDecellularized materials derived from TSP2-KO mice promote enhanced neovascularization and integration in diabetic wounds
Morris AH, Stamer DK, Kunkemoeller B, Chang J, Xing H, Kyriakides TR. Decellularized materials derived from TSP2-KO mice promote enhanced neovascularization and integration in diabetic wounds. Biomaterials 2018, 169: 61-71. PMID: 29631168, PMCID: PMC5933884, DOI: 10.1016/j.biomaterials.2018.03.049.Peer-Reviewed Original ResearchConceptsUltimate tensile strengthECM-based materialsTensile strengthMechanical testingDecellularized materialsElastic modulusSynthetic biomaterialsIntact slabsMatrix propertiesExtracellular matrix propertiesCell-derived ECMBiologic scaffoldsElectron microscopyEngineering controlsMaterialsWtEfficient integrationEnhanced vascularizationModulusPromigratory propertiesScaffoldsPropertiesBiomaterialsEnhanced remodelingGreater cell migration
2015
Cryopreserved human amniotic membrane and a bioinspired underwater adhesive to seal and promote healing of iatrogenic fetal membrane defect sites
Papanna R, Mann LK, Tseng SC, Stewart RJ, Kaur SS, Swindle MM, Kyriakides TR, Tatevian N, Moise KJ. Cryopreserved human amniotic membrane and a bioinspired underwater adhesive to seal and promote healing of iatrogenic fetal membrane defect sites. Placenta 2015, 36: 888-894. PMID: 26059341, PMCID: PMC4529759, DOI: 10.1016/j.placenta.2015.05.015.Peer-Reviewed Original Research
1999
Accelerated Wound Healing in Mice With a Disruption of the Thrombospondin 2 Gene
Kyriakides T, Tam J, Bornstein P. Accelerated Wound Healing in Mice With a Disruption of the Thrombospondin 2 Gene. Journal Of Investigative Dermatology 1999, 113: 782-787. PMID: 10571734, DOI: 10.1046/j.1523-1747.1999.00755.x.Peer-Reviewed Original ResearchConceptsGranulation tissueThrombospondin-2Late woundsControl woundsWound healingExtent of angiogenesisRe-epithelization rateAbnormal collagen fibrilsVascular densityLess scarringRete pegsHistologic analysisExcisional woundsControl epitheliumSuch woundsThrombospondin 2 geneTotal cellular contentAccelerated wound healingElevated levelsHealing processMiceWoundsBiopsy punchEarly woundsHealing