1994
25 Electron Microscopic Double and Triple Labeling Immunocytochemistry in Elucidation of Synaptological Interactions between Ovarian Steroid-Sensitive Neurons and Circuits
Leranth C, Naftolin F, Shanabrough M, Horvath T. 25 Electron Microscopic Double and Triple Labeling Immunocytochemistry in Elucidation of Synaptological Interactions between Ovarian Steroid-Sensitive Neurons and Circuits. International Review Of Neurobiology 1994, 22: 403-434. DOI: 10.1016/b978-0-12-185292-4.50031-0.Peer-Reviewed Original ResearchDouble immunostaining techniqueHormone-releasing hormone (LHRH) neuronsSteroid-sensitive neuronsImmunostaining techniquesRetrograde tracer techniqueTriple-labeling immunocytochemistryHormone neuronsDouble immunostainingTissue antigensDifferent anestheticsAnatomical basisSame satisfactory resultsTissue preparationsNeuronsElectron microscopicElectron-dense labelKetaminePentobarbitalAnestheticsImmunostainingAntigenDegenerationMethoxyfluraneChloropentAntibodies
1992
GABAergic and catecholaminergic innervation of mediobasal hypothalamic β-endorphin cells projecting to the medial preoptic area
Horvath TL, Naftolin F, Leranth C. GABAergic and catecholaminergic innervation of mediobasal hypothalamic β-endorphin cells projecting to the medial preoptic area. Neuroscience 1992, 51: 391-399. PMID: 1281529, DOI: 10.1016/0306-4522(92)90323-t.Peer-Reviewed Original ResearchConceptsMedial preoptic areaPreoptic areaHormone-producing cellsArcuate nucleusHypothalamic beta-endorphin neuronsHormone-releasing hormone (LHRH) neuronsBeta-endorphin neuronsDirect estrogen actionHormone-containing neuronsVentromedial arcuate nucleusRetrograde tracer horseradish peroxidasePro-opiomelanocortin (POMC) neuronsCellular estrogen receptorsPro-opiomelanocortin cellsTracer horseradish peroxidaseΒ-endorphin cellsHormone neuronsImmunoreactive neuronsCatecholaminergic innervationCatecholaminergic neuronsRetrograde tracingSynaptic contactsEstrogen actionCatecholamine fibersAxon terminals