2022
Dysregulated stem cell niches and altered lymphocyte recirculation cause B and T cell lymphopenia in WHIM syndrome
Zehentmeier S, Lim VY, Ma Y, Fossati J, Ito T, Jiang Y, Tumanov AV, Lee HJ, Dillinger L, Kim J, Csomos K, Walter JE, Choi J, Pereira JP. Dysregulated stem cell niches and altered lymphocyte recirculation cause B and T cell lymphopenia in WHIM syndrome. Science Immunology 2022, 7: eabo3170. PMID: 36149943, PMCID: PMC9614684, DOI: 10.1126/sciimmunol.abo3170.Peer-Reviewed Original ResearchConceptsSecondary lymphoid organsWHIM syndromeMesenchymal stem cellsInterleukin-7B lymphopeniaBone marrowBM mesenchymal stem cellsT cell numbersIL-7 productionT-cell lymphopeniaLymphotoxin beta receptorEarly progenitor stageLymphoid organsCell lymphopeniaMouse modelBeta receptorsB cellsB cell developmentLymphopeniaStromal cellsLeukocyte retentionSyndromeGOF mutationsLymphopoietic activityCritical pathways
2020
Bone Marrow Endothelial Cells Take Up Blood-Borne Immune Complexes via Fcγ Receptor IIb2 in an Erythropoietin-Dependent Manner.
Ito T, Kometani K, Minato N, Hamazaki Y. Bone Marrow Endothelial Cells Take Up Blood-Borne Immune Complexes via Fcγ Receptor IIb2 in an Erythropoietin-Dependent Manner. The Journal Of Immunology 2020, 205: 2008-2015. PMID: 32907997, DOI: 10.4049/jimmunol.1901101.Peer-Reviewed Original ResearchConceptsImmune complexesHealthy miceBone marrowUptake of ICsEndothelial cellsAdministration of erythropoietinSoluble immune complexesLiver reticuloendothelial systemSinusoidal endothelial cellsBone marrow endothelial cellsMarrow endothelial cellsEPO administrationKupffer cellsTransgenic miceAnemic conditionBM erythroblastsReticuloendothelial systemSinusoidal vasculatureMiceBMECsEPO receptorHematopoietic cellsAdministrationEPOHematopoietic nicheSelective expression of claudin-5 in thymic endothelial cells regulates the blood–thymus barrier and T-cell export
Nagatake T, Zhao Y, Ito T, Itoh M, Kometani K, Furuse M, Saika A, Node E, Kunisawa J, Minato N, Hamazaki Y. Selective expression of claudin-5 in thymic endothelial cells regulates the blood–thymus barrier and T-cell export. International Immunology 2020, 33: 171-182. PMID: 33038259, PMCID: PMC7936066, DOI: 10.1093/intimm/dxaa069.Peer-Reviewed Original ResearchConceptsCortico-medullary junctionBlood-thymus barrierEndothelial cellsClaudin-5Biotin tracerCortical vasculatureBlood-borne moleculesCentral tolerance inductionT cell exportThymic endothelial cellsEC cell linesSphingosine-1-PhosphateT cell developmentDendritic cellsThymic cortexTolerance inductionTrans-endothelial resistanceThymocyte egressVascular permeabilityThymic microenvironmentMature thymocytesMedullaBlood circulationSelective expressionMedullary parenchyma
2017
Bone marrow endothelial cells induce immature and mature B cell egress in response to erythropoietin
Ito T, Hamazaki Y, Takaori-Kondo A, Minato N. Bone marrow endothelial cells induce immature and mature B cell egress in response to erythropoietin. Cell Structure And Function 2017, 42: 17018. PMID: 29070774, DOI: 10.1247/csf.17018.Peer-Reviewed Original ResearchConceptsBone marrow endothelial cellsMarrow endothelial cellsStromal cellsEndothelial cellsEmergency hematopoiesisBone marrow stromal cellsMature B cellsPhosphorylation of STAT5Immature B cellsSpecific lineagesB cell egressCxcl12 transcriptionMarrow stromal cellsB cell retentionErythropoietin receptorBone marrow microenvironmentBone marrowHematopoietic stemB cellsPrecursor cellsPhenylhydrazine-induced hemolytic anemiaExpression levelsErythropoietin stimulationMesenchymal stromal cellsMarrow microenvironment
2014
In vivo imaging reveals PKA regulation of ERK activity during neutrophil recruitment to inflamed intestines
Mizuno R, Kamioka Y, Kabashima K, Imajo M, Sumiyama K, Nakasho E, Ito T, Hamazaki Y, Okuchi Y, Sakai Y, Kiyokawa E, Matsuda M. In vivo imaging reveals PKA regulation of ERK activity during neutrophil recruitment to inflamed intestines. Journal Of Experimental Medicine 2014, 211: 1123-1136. PMID: 24842369, PMCID: PMC4042632, DOI: 10.1084/jem.20132112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Inflammatory Agents, Non-SteroidalBenzamidesCell AdhesionCell MovementCyclic AMP-Dependent Protein KinasesDiphenylamineEndothelial CellsEnteritisExtracellular Signal-Regulated MAP KinasesFemaleFluorescence Resonance Energy TransferGreen Fluorescent ProteinsIntestine, SmallMaleMethyl EthersMiceMice, Inbred C57BLMice, TransgenicMicroscopy, Fluorescence, MultiphotonNaphthalenesNeutrophil InfiltrationNeutrophilsPhenylbutyratesReceptors, Prostaglandin E, EP4 SubtypeTime-Lapse ImagingConceptsNonsteroidal antiinflammatory drugsExtracellular signal-regulated kinaseChemical mediatorsNeutrophil recruitmentEndothelial cellsInterstitial tissueSuch chemical mediatorsInflamed intestinal tissueProstaglandin receptor EP4Migration of neutrophilsProtein kinase ANeutrophil recruitment cascadeInflamed intestineEP4 agonistReceptor EP4ERK activityEP4 receptorsAntiinflammatory drugsIntravenous administrationInflammatory sitesIntestinal tissueTransgenic miceSignal-regulated kinaseNeutrophilsRecruitment cascade