In vivo imaging reveals PKA regulation of ERK activity during neutrophil recruitment to inflamed intestines
Mizuno R, Kamioka Y, Kabashima K, Imajo M, Sumiyama K, Nakasho E, Ito T, Hamazaki Y, Okuchi Y, Sakai Y, Kiyokawa E, Matsuda M. In vivo imaging reveals PKA regulation of ERK activity during neutrophil recruitment to inflamed intestines. Journal Of Experimental Medicine 2014, 211: 1123-1136. PMID: 24842369, PMCID: PMC4042632, DOI: 10.1084/jem.20132112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Inflammatory Agents, Non-SteroidalBenzamidesCell AdhesionCell MovementCyclic AMP-Dependent Protein KinasesDiphenylamineEndothelial CellsEnteritisExtracellular Signal-Regulated MAP KinasesFemaleFluorescence Resonance Energy TransferGreen Fluorescent ProteinsIntestine, SmallMaleMethyl EthersMiceMice, Inbred C57BLMice, TransgenicMicroscopy, Fluorescence, MultiphotonNaphthalenesNeutrophil InfiltrationNeutrophilsPhenylbutyratesReceptors, Prostaglandin E, EP4 SubtypeTime-Lapse ImagingConceptsNonsteroidal antiinflammatory drugsExtracellular signal-regulated kinaseChemical mediatorsNeutrophil recruitmentEndothelial cellsInterstitial tissueSuch chemical mediatorsInflamed intestinal tissueProstaglandin receptor EP4Migration of neutrophilsProtein kinase ANeutrophil recruitment cascadeInflamed intestineEP4 agonistReceptor EP4ERK activityEP4 receptorsAntiinflammatory drugsIntravenous administrationInflammatory sitesIntestinal tissueTransgenic miceSignal-regulated kinaseNeutrophilsRecruitment cascade