2016
Probing the mechanisms underlying human diseases in making ribosomes.
Farley KI, Baserga SJ. Probing the mechanisms underlying human diseases in making ribosomes. Biochemical Society Transactions 2016, 44: 1035-44. PMID: 27528749, PMCID: PMC5360156, DOI: 10.1042/bst20160064.Peer-Reviewed Original ResearchBox C/D sRNA stem ends act as stabilizing anchors for box C/D di-sRNPs
Yip WS, Shigematsu H, Taylor DW, Baserga SJ. Box C/D sRNA stem ends act as stabilizing anchors for box C/D di-sRNPs. Nucleic Acids Research 2016, 44: 8976-8989. PMID: 27342279, PMCID: PMC5062973, DOI: 10.1093/nar/gkw576.Peer-Reviewed Original ResearchConceptsBox C/DArchaeal box C/D sRNPsBox C/D sRNAsBox C/D sRNPRibosomal RNA modificationsElectron microscopy modelCryo-EM reconstructionCellular organismsRibosome functionRNA modificationsSRNAsSRNPModification typesStructural techniquesEukaryaPotential interactionsStemArchaeaStructural analysisStem endClose proximityOrganismsNuclear magnetic resonance studiesCatalyseInteraction
2001
An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association.
Wormsley S, Samarsky D, Fournier M, Baserga S. An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association. RNA 2001, 7: 904-19. PMID: 11421365, PMCID: PMC1370138, DOI: 10.1017/s1355838201010238.Peer-Reviewed Original ResearchConceptsU3 snoRNAU3 snoRNPYeast U3 snoRNAU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinSmall nucleolar RNAsStretch of nucleotidesHinge regionBase-pairing interactionsRRNA transcriptsNucleolar RNAsNucleolar ribonucleoproteinConserved elementSequence elementsProtein componentsSnoRNAsProcessing reactionsBase pairsMpp10pBase pairingSnoRNPsFunctional centersRRNACritical roleProtein
1998
The U14 snoRNA is required for 2'-O-methylation of the pre-18S rRNA in Xenopus oocytes.
Dunbar D, Baserga S. The U14 snoRNA is required for 2'-O-methylation of the pre-18S rRNA in Xenopus oocytes. RNA 1998, 4: 195-204. PMID: 9570319, PMCID: PMC1369608.Peer-Reviewed Original Research
1992
Beta-globin nonsense mutation: deficient accumulation of mRNA occurs despite normal cytoplasmic stability.
Baserga S, Benz E. Beta-globin nonsense mutation: deficient accumulation of mRNA occurs despite normal cytoplasmic stability. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 2935-2939. PMID: 1557399, PMCID: PMC48778, DOI: 10.1073/pnas.89.7.2935.Peer-Reviewed Original ResearchConceptsMRNA metabolismHeterologous transfection systemRNA polymerase IIHuman beta-globin geneMetabolism of mRNAAmber nonsense mutationsAnalysis of transcriptionAccumulation of mRNARNA expression studiesBeta-globin genePolymerase IIMRNA polyadenylationCytoplasmic stabilityMRNA stabilityExpression studiesPermanent cell linesProtein synthesisTransfection systemMediterranean populationsNonsense mutationSplicing accuracyMutationsDeficient accumulationCell linesMRNA
1988
Detecting small mutations in expressed genes by a combination of S1 nuclease and RNase A
Atweh G, Baserga S, Brickner H. Detecting small mutations in expressed genes by a combination of S1 nuclease and RNase A. Nucleic Acids Research 1988, 16: 8709-8709. PMID: 2843823, PMCID: PMC338588, DOI: 10.1093/nar/16.17.8709.Peer-Reviewed Original ResearchNonsense mutations in the human beta-globin gene affect mRNA metabolism.
Baserga S, Benz E. Nonsense mutations in the human beta-globin gene affect mRNA metabolism. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 2056-2060. PMID: 3353367, PMCID: PMC279927, DOI: 10.1073/pnas.85.7.2056.Peer-Reviewed Original ResearchConceptsHeterologous transfection systemBeta-globin mRNABeta-globin geneNonsense mutationHuman beta-globin geneTransfection systemTranslation termination codonPeripheral blood cellsHuman beta-globin mRNATranslation termination mutationsMammalian mRNAsMRNA metabolismBeta 17Steady-state levelsTermination codonMRNA accumulationNormal levelsTypes of mutationsTermination mutationsBlood cellsMissense mutationsGenesHuman alphaMutationsZero-thalassemia