Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA
Zhang J, McCann KL, Qiu C, Gonzalez LE, Baserga SJ, Hall TM. Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA. Nature Communications 2016, 7: 13085. PMID: 27725644, PMCID: PMC5062617, DOI: 10.1038/ncomms13085.Peer-Reviewed Original ResearchConceptsEukaryotic ribosome biogenesisCorrect subcellular locationRibosome assembly factorsPre-ribosomal RNAPumilio repeatsRibosome biogenesisHuman ribosomopathiesAssembly factorsBiogenesis factorsRepeat proteinsMature rRNASubcellular locationNop9RNA complexCleavage siteRRNATimely cleavageProteinStructural featuresFinal processing stepRibosomopathiesBiogenesisCleavageYeastNucleaseBox C/D sRNA stem ends act as stabilizing anchors for box C/D di-sRNPs
Yip WS, Shigematsu H, Taylor DW, Baserga SJ. Box C/D sRNA stem ends act as stabilizing anchors for box C/D di-sRNPs. Nucleic Acids Research 2016, 44: 8976-8989. PMID: 27342279, PMCID: PMC5062973, DOI: 10.1093/nar/gkw576.Peer-Reviewed Original ResearchConceptsBox C/DArchaeal box C/D sRNPsBox C/D sRNAsBox C/D sRNPRibosomal RNA modificationsElectron microscopy modelCryo-EM reconstructionCellular organismsRibosome functionRNA modificationsSRNAsSRNPModification typesStructural techniquesEukaryaPotential interactionsStemArchaeaStructural analysisStem endClose proximityOrganismsNuclear magnetic resonance studiesCatalyseInteraction