2011
The initial U3 snoRNA:pre‐rRNA base‐pairing interaction required for pre‐18S rRNA folding revealed by in vivo chemical probing
Baserga S, Dutca L, Gallagher J. The initial U3 snoRNA:pre‐rRNA base‐pairing interaction required for pre‐18S rRNA folding revealed by in vivo chemical probing. The FASEB Journal 2011, 25: 704.1-704.1. DOI: 10.1096/fasebj.25.1_supplement.704.1.Peer-Reviewed Original ResearchU3 snoRNABase-pairing interactionsSmall subunitRibosomal subunitU3 small nucleolar RNAPre-rRNA foldingVivo chemical probingSmall nucleolar RNAsSmall ribosomal subunitRRNA foldingEukaryotic nucleolusRibosomal proteinsU3 snoRNPCytoplasmic ribosomesNucleolar RNAsGenetic manipulationS. cerevisiaeChemical probingSnoRNAsVivo chemicalRRNAEssential processSubunitsFoldingNucleotide reactivity
2005
Autoantibody Recognition of Macromolecular Structures and Their Subunits
Champion E, Baserga S. Autoantibody Recognition of Macromolecular Structures and Their Subunits. 2005, 379-417. DOI: 10.1002/3527607854.ch17.Peer-Reviewed Original ResearchSm monoclonal antibodyRNA polymerase IBox C/D small nucleolar RNAsPre-mRNA splicingRNase MRPSmall nucleolar RNAsRNase PPolymerase IU3 snoRNASubcellular localizationMolecular markersProtein componentsNucleolar RNAsHuman exosomeNew functionsPolymyositis-scleroderma overlap syndromeCurrent understandingSnRNPsSnoRNAsSplicingFibrillarinNucleolarMonoclonal antibodiesExosomesHUBF
2001
An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association.
Wormsley S, Samarsky D, Fournier M, Baserga S. An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association. RNA 2001, 7: 904-19. PMID: 11421365, PMCID: PMC1370138, DOI: 10.1017/s1355838201010238.Peer-Reviewed Original ResearchConceptsU3 snoRNAU3 snoRNPYeast U3 snoRNAU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinSmall nucleolar RNAsStretch of nucleotidesHinge regionBase-pairing interactionsRRNA transcriptsNucleolar RNAsNucleolar ribonucleoproteinConserved elementSequence elementsProtein componentsSnoRNAsProcessing reactionsBase pairsMpp10pBase pairingSnoRNPsFunctional centersRRNACritical roleProtein
2000
The genes for small nucleolar RNAs in Trypanosoma brucei are organized in clusters and are transcribed as a polycistronic RNA
Dunbar D, Chen A, Wormsley S, Baserga S. The genes for small nucleolar RNAs in Trypanosoma brucei are organized in clusters and are transcribed as a polycistronic RNA. Nucleic Acids Research 2000, 28: 2855-2861. PMID: 10908346, PMCID: PMC102681, DOI: 10.1093/nar/28.15.2855.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacteriophage P1Base SequenceBlotting, SouthernChromosome MappingCloning, MolecularDNA Restriction EnzymesGenesMolecular Sequence DataMultigene FamilyPolymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, ProtozoanRNA, Small NucleolarTandem Repeat SequencesTranscription, GeneticTrypanosoma brucei bruceiFibrillarin-associated Box C/D Small Nucleolar RNAs inTrypanosoma brucei SEQUENCE CONSERVATION AND IMPLICATIONS FOR 2′-O-RIBOSE METHYLATION OF rRNA*
Dunbar D, Wormsley S, Lowe T, Baserga S. Fibrillarin-associated Box C/D Small Nucleolar RNAs inTrypanosoma brucei SEQUENCE CONSERVATION AND IMPLICATIONS FOR 2′-O-RIBOSE METHYLATION OF rRNA*. Journal Of Biological Chemistry 2000, 275: 14767-14776. PMID: 10747997, DOI: 10.1074/jbc.m001180200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceChromosomal Proteins, Non-HistoneCloning, MolecularConserved SequenceDNA PrimersMethylationMolecular Sequence DataPrecipitin TestsRabbitsRiboseRNA, ProtozoanRNA, RibosomalRNA, Small NuclearSequence Homology, Amino AcidSequence Homology, Nucleic AcidTrypanosoma brucei bruceiConceptsRibose methylationBox C/D snoRNAsMethylation guide snoRNAsSmall nucleolar RNAsAncient eukaryotesBrucei extractsComparative genomicsEarly eukaryotesGuide snoRNAsD snoRNAsFibrillarin proteinSequence conservationSnoRNA sequencesNucleolar RNAsNucleotides upstreamBox CBox DSnoRNAsT. bruceiCommon proteinsEukaryotesFibrillarinRNA 1MethylationRRNA
1998
M Phase Phosphoprotein 10 Is a Human U3 Small Nucleolar Ribonucleoprotein Component
Westendorf J, Konstantinov K, Wormsley S, Shu M, Matsumoto-Taniura N, Pirollet F, Klier F, Gerace L, Baserga S. M Phase Phosphoprotein 10 Is a Human U3 Small Nucleolar Ribonucleoprotein Component. Molecular Biology Of The Cell 1998, 9: 437-449. PMID: 9450966, PMCID: PMC25272, DOI: 10.1091/mbc.9.2.437.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCell FractionationCell NucleolusChromosomal Proteins, Non-HistoneCloning, MolecularDactinomycinDNA, ComplementaryHeLa CellsHumansIsoelectric PointMitosisMolecular Sequence DataMolecular WeightNuclear ProteinsPhosphoproteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRNA, Small NuclearSequence Analysis, DNASpecies SpecificityConceptsM-phase phosphoproteinsPrenucleolar bodiesBox C/D snoRNAsU3 small nucleolar RNASmall nucleolar RNAsIsolation of cDNAsHuman U3D snoRNAsRRNA processingNucleolar proteinsP80-coilinNovel proteinNucleolar functionU3 snoRNAChromosome surfaceNucleolar RNAsCell fractionationMpp10FibrillarinInterphase cellsCell cycleRibonucleoprotein componentsM phaseProteinSnoRNAs
1997
Mpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors.
Baserga S, Agentis T, Wormsley S, Dunbar D, Lee S. Mpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors. Nucleic Acids Symposium Series 1997, 64-7. PMID: 9478208.Peer-Reviewed Original ResearchConceptsPre-rRNA processingNew protein componentsU3 snoRNPProtein componentsU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinEssential genesSlow growthConditional promoterU3 snoRNAMpp10pNucleolar ribonucleoproteinRRNA precursorProcessing eventsYeastSnoRNPsMpp10ProteinModel systemDegrees C. AnalysisC. AnalysisSnoRNAsMutantsRibonucleoproteinSaccharomyces