2020
Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
Micali N, Kim SK, Diaz-Bustamante M, Stein-O’Brien G, Seo S, Shin JH, Rash BG, Ma S, Wang Y, Olivares NA, Arellano JI, Maynard KR, Fertig EJ, Cross AJ, Bürli RW, Brandon NJ, Weinberger DR, Chenoweth JG, Hoeppner DJ, Sestan N, Rakic P, Colantuoni C, McKay RD. Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates. Cell Reports 2020, 31: 107599. PMID: 32375049, PMCID: PMC7357345, DOI: 10.1016/j.celrep.2020.107599.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationCells, CulturedEmbryonic Stem CellsHumansInduced Pluripotent Stem CellsNeural Stem CellsNeurogenesisNeuronsSignal TransductionConceptsNeural stem cellsNeuronal fateProliferative neural stem cellsStem cellsPluripotent linesTelencephalic fateRNA sequencingLineage tracingHuman neural stem cellsGlutamatergic excitatory neuronsMonkey brain sectionsNeuronal trajectoriesCell imagingCortical excitatoryCerebral cortexFateExcitatory neuronsBrain sectionsHuman telencephalonNeuropsychiatric disordersAcute transitionPluripotencyCellsCortexSequencingDissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs
Burke EE, Chenoweth JG, Shin JH, Collado-Torres L, Kim SK, Micali N, Wang Y, Colantuoni C, Straub RE, Hoeppner DJ, Chen HY, Sellers A, Shibbani K, Hamersky GR, Diaz Bustamante M, Phan BN, Ulrich WS, Valencia C, Jaishankar A, Price AJ, Rajpurohit A, Semick SA, Bürli RW, Barrow JC, Hiler DJ, Page SC, Martinowich K, Hyde TM, Kleinman JE, Berman KF, Apud JA, Cross AJ, Brandon NJ, Weinberger DR, Maher BJ, McKay RDG, Jaffe AE. Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs. Nature Communications 2020, 11: 462. PMID: 31974374, PMCID: PMC6978526, DOI: 10.1038/s41467-019-14266-z.Peer-Reviewed Original ResearchConceptsHuman induced pluripotent stem cellsNeural precursor cellsExpression dataSingle-cell expression dataNeuronal differentiationSequencing read alignmentsInduced pluripotent stem cellsEarly neuronal differentiationPluripotent stem cellsTranscriptomic resourcesIPSC donorNeuronal culturesSubclonal linesNeural differentiationTranscriptomic signaturesHuman neural precursor cellsNeuronal cellsStem cellsPrecursor cellsCell sortingGlobal patternsPowerful modelSubset of neuronsRead alignmentDifferentiation
2018
Nanotopographical regulation of pancreatic islet-like cluster formation from human pluripotent stem cells using a gradient-pattern chip
Kim J, Park B, Kim S, Lee D, Lee G, Kim D, Choi B, Lee K, Kim J. Nanotopographical regulation of pancreatic islet-like cluster formation from human pluripotent stem cells using a gradient-pattern chip. Acta Biomaterialia 2018, 95: 337-347. PMID: 30529081, DOI: 10.1016/j.actbio.2018.12.011.Peer-Reviewed Original ResearchConceptsHuman pluripotent stem cellsPancreatic islet-like clustersStem cell fatePluripotent stem cellsIslet-like clustersCell fateSpecific functional cell typesCell typesPancreatic endocrine progenitorsStem cellsStem cell nichePancreatic islet developmentFunctional cell typesSpecific cell typesPancreatic cellsDevelopmental regulatorsEndocrine progenitorsCell nicheMechanical cuesPancreatic β-cellsPolyhormonal cells
2015
Pancreatic Islet-Like Three-Dimensional Aggregates Derived From Human Embryonic Stem Cells Ameliorate Hyperglycemia in Streptozotocin-Induced Diabetic Mice
Shim J, Kim J, Han J, An S, Jang Y, Son J, Woo D, Kim S, Kim J. Pancreatic Islet-Like Three-Dimensional Aggregates Derived From Human Embryonic Stem Cells Ameliorate Hyperglycemia in Streptozotocin-Induced Diabetic Mice. Cell Transplantation 2015, 24: 2155-2168. PMID: 25397866, DOI: 10.3727/096368914x685438.Peer-Reviewed Original ResearchConceptsDiabetic miceHuman embryonic stem cellsClinical islet transplantationBlood glucose levelsMature pancreatic endocrine cellsPancreatic endocrine cellsStem cellsInhibition of NotchAmeliorate hyperglycemiaIslet transplantationPancreatic hormonesGlucose levelsPancreatic endodermDonor isletsPancreatic featuresActivin APharmacological drugsEndocrine cellsIncreased expressionThree-dimensional aggregatesExpression of Neurog3Transient upregulationProgenitor cellsRetinoic acidActivin βB
2012
Sox2 Acts through Sox21 to Regulate Transcription in Pluripotent and Differentiated Cells
Kuzmichev A, Kim S, D’Alessio A, Chenoweth J, Wittko I, Campanati L, McKay R. Sox2 Acts through Sox21 to Regulate Transcription in Pluripotent and Differentiated Cells. Current Biology 2012, 22: 1705-1710. PMID: 22902753, DOI: 10.1016/j.cub.2012.07.013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCDX2 Transcription FactorCell DifferentiationCell LineColonic NeoplasmsFibroblastsGene Expression RegulationHomeodomain ProteinsIntestinal MucosaIntestinesMiceMice, TransgenicOctamer Transcription Factor-3Pluripotent Stem CellsSOXB1 Transcription FactorsSOXB2 Transcription FactorsTranscription FactorsTranscription, GeneticTranscriptional ActivationConceptsStem cellsImportant transcriptional regulatorStem cell renewalIntestinal stem cellsAdult stem cellsColon cancer cellsEndodermal identityPluripotent stem cellsEffect of SOX2Gene familyCell identityPluripotent stateTranscriptional partnersTranscriptional regulatorsTranscriptional inductionTranscription factorsMaster regulatorTranscriptional actionGene expressionSox21Differentiated cellsCell renewalSOX2General mediatorCell types
2011
Direct and Indirect Contribution of Human Embryonic Stem Cell–Derived Hepatocyte-Like Cells to Liver Repair in Mice
Woo D, Kim S, Lim H, Heo J, Park H, Kang G, Kim S, You H, Hoeppner D, Kim Y, Kwon H, Choi T, Lee J, Hong S, Song K, Ahn E, Chenoweth J, Tesar P, McKay R, Kim J. Direct and Indirect Contribution of Human Embryonic Stem Cell–Derived Hepatocyte-Like Cells to Liver Repair in Mice. Gastroenterology 2011, 142: 602-611. PMID: 22138358, DOI: 10.1053/j.gastro.2011.11.030.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCarbon TetrachlorideCell DifferentiationCell ProliferationCell SeparationCells, CulturedChemical and Drug Induced Liver InjuryCoculture TechniquesDisease Models, AnimalEmbryonic Stem CellsHepatocytesHumansImmunohistochemistryInduced Pluripotent Stem CellsLaser Capture MicrodissectionLithium ChlorideLiverLiver RegenerationMass SpectrometryMiceMice, Inbred BALB CMice, NudeMicroscopy, ElectronNeovascularization, PhysiologicPolymerase Chain ReactionProteomicsTime FactorsWound HealingConceptsHepatocyte-like cellsLiver repairLiver tissueCell replacementEndogenous liver regenerationAcute liver injuryDirect cell replacementHost liver tissueStem cellsLiver of miceIndocyanine green stainingHuman embryonic stem cell-derived cellsEmbryonic stem cell-derived cellsCell-derived signalsHost tissue repairStem cell-derived cellsStem cell-derived hepatocyte-like cellsCell-derived hepatocyte-like cellsLiver injuryCell-derived cellsPolymerase chain reactionCell graftsIntraperitoneal injectionHepatic featuresTrophic factorsBiochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies
Lim H, Han J, Woo D, Kim S, Kim S, Kang H, Kim J. Biochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies. Molecules And Cells 2011, 31: 123-132. PMID: 21347709, PMCID: PMC3932683, DOI: 10.1007/s10059-011-0016-8.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsMild hypoxiaEmbryonic stem cellsEmbryoid bodiesLong-term cultureBrdU incorporationCell fate determinationDifferentiating embryoid bodiesExpression of markersCaspase-3 immunostainingStem cellsPercentage of cellsEmbryonic germ layersActive caspase-3Hematoxylineosin stainingMammalian reproductive tract
2009
Differential cytotoxic effects of mono-(2-ethylhexyl) phthalate on blastomere-derived embryonic stem cells and differentiating neurons
Lim C, Kim S, Ko D, Cho J, Jun J, An S, Han J, Kim J, Yoon Y. Differential cytotoxic effects of mono-(2-ethylhexyl) phthalate on blastomere-derived embryonic stem cells and differentiating neurons. Toxicology 2009, 264: 145-154. PMID: 19720108, DOI: 10.1016/j.tox.2009.08.015.Peer-Reviewed Original ResearchDifferentiation of Neural Progenitor Cells in a Microfluidic Chip‐Generated Cytokine Gradient
Park J, Kim S, Woo D, Lee E, Kim J, Lee S. Differentiation of Neural Progenitor Cells in a Microfluidic Chip‐Generated Cytokine Gradient. Stem Cells 2009, 27: 2646-2654. PMID: 19711444, DOI: 10.1002/stem.202.Peer-Reviewed Original ResearchConceptsCell typesNeural progenitorsHuman embryonic stem cellsEarly embryonic developmentStem cellsEmbryonic stem cellsCytokine gradientsEnriched populationNeural progenitor cellsPrimitive stem cellsEmbryonic developmentSignaling moleculesDiverse tissuesCell body clustersProgenitor cellsNeurite bundlesGrowth factorProgenitorsCell-friendly microenvironmentCellsDifferentiationExogenous cytokinesImportant roleBody clustersSpatial gradients
2007
Directed differentiation of human embryonic stem cells towards a pancreatic cell fate
Shim J, Kim S, Woo D, Kim S, Oh C, McKay R, Kim J. Directed differentiation of human embryonic stem cells towards a pancreatic cell fate. Diabetologia 2007, 50: 1228-1238. PMID: 17457565, DOI: 10.1007/s00125-007-0634-z.Peer-Reviewed Original ResearchMeSH KeywordsActivinsAnimalsCell Culture TechniquesCell DifferentiationDiabetes Mellitus, ExperimentalEmbryonic Stem CellsGene Expression RegulationHomeodomain ProteinsHumansMaleMiceMice, Inbred BALB CMice, NudePancreasReverse Transcriptase Polymerase Chain ReactionStem Cell TransplantationTrans-ActivatorsTransplantation, HeterologousTretinoinConceptsHuman embryonic stem cellsPdx1-positive cellsForkhead box protein A2Embryonic stem cellsEndodermal cellsCaudal-type homeobox transcription factor 2Differentiation of hESCsCell typesPancreatic cell fateRetinoic acidStem cellsMature cell typesEmbryonic germ layersEmbryoid body formationPancreatic cell typesBox protein A2Transcription factor 2Vivo pancreatic developmentEpigenetic signalsCell fateEctodermal differentiationDuodenal homeobox 1Pancreatic fateGene 17Gene expressionComparative Analysis of the Developmental Competence of Three Human Embryonic Stem Cell Lines in Vitro
Kim S, Kim B, Gil J, Kim S, Kim J. Comparative Analysis of the Developmental Competence of Three Human Embryonic Stem Cell Lines in Vitro. Molecules And Cells 2007, 23: 49-56. PMID: 17464211, DOI: 10.1016/s1016-8478(23)07388-0.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersCell DifferentiationCell LineCell LineageEctodermEmbryonic Stem CellsEndodermGene Expression RegulationHumansMesodermPluripotent Stem CellsConceptsHuman embryonic stem cellsHESC linesHuman embryonic stem cell linesEmbryonic stem cell linesPrimordial germ cellsDevelopmental potentialEmbryonic stem cellsEmbryonic germ layersEmbryoid body formationSpecific cell typesDevelopmental competenceStem cell linesCell replacement therapyExtraembryonic tissuesStem cell technologyGerm layersTerminal differentiationGerm cellsBody formationPluripotency markersCell typesNeuronal cellsTelomerase activityStem cellsHSF6
2006
Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells
Kim B, Kim S, Shim J, Woo D, Gil J, Kim S, Kim J. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells. FEBS Letters 2006, 580: 5869-5874. PMID: 17027979, DOI: 10.1016/j.febslet.2006.09.053.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsVascular endothelial growth factorGerm layer formationEmbryonic stem cellsEmbryoid bodiesEndothelial growth factorNeurogenic effectsGrowth factorStem cellsEffects of VEGFNeuroectodermal genesVascular endothelial cellsHESC differentiationNeural rosettesDopaminergic neuronsGene expressionNeural progenitorsTerminal differentiationVEGF treatmentPotent mitogenEndothelial cellsNeuroectodermal differentiationDifferentiationCellsExpression