2015
Pancreatic Islet-Like Three-Dimensional Aggregates Derived From Human Embryonic Stem Cells Ameliorate Hyperglycemia in Streptozotocin-Induced Diabetic Mice
Shim J, Kim J, Han J, An S, Jang Y, Son J, Woo D, Kim S, Kim J. Pancreatic Islet-Like Three-Dimensional Aggregates Derived From Human Embryonic Stem Cells Ameliorate Hyperglycemia in Streptozotocin-Induced Diabetic Mice. Cell Transplantation 2015, 24: 2155-2168. PMID: 25397866, DOI: 10.3727/096368914x685438.Peer-Reviewed Original ResearchConceptsDiabetic miceHuman embryonic stem cellsClinical islet transplantationBlood glucose levelsMature pancreatic endocrine cellsPancreatic endocrine cellsStem cellsInhibition of NotchAmeliorate hyperglycemiaIslet transplantationPancreatic hormonesGlucose levelsPancreatic endodermDonor isletsPancreatic featuresActivin APharmacological drugsEndocrine cellsIncreased expressionThree-dimensional aggregatesExpression of Neurog3Transient upregulationProgenitor cellsRetinoic acidActivin βB
2011
Biochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies
Lim H, Han J, Woo D, Kim S, Kim S, Kang H, Kim J. Biochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies. Molecules And Cells 2011, 31: 123-132. PMID: 21347709, PMCID: PMC3932683, DOI: 10.1007/s10059-011-0016-8.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsMild hypoxiaEmbryonic stem cellsEmbryoid bodiesLong-term cultureBrdU incorporationCell fate determinationDifferentiating embryoid bodiesExpression of markersCaspase-3 immunostainingStem cellsPercentage of cellsEmbryonic germ layersActive caspase-3Hematoxylineosin stainingMammalian reproductive tract
2007
Directed differentiation of human embryonic stem cells towards a pancreatic cell fate
Shim J, Kim S, Woo D, Kim S, Oh C, McKay R, Kim J. Directed differentiation of human embryonic stem cells towards a pancreatic cell fate. Diabetologia 2007, 50: 1228-1238. PMID: 17457565, DOI: 10.1007/s00125-007-0634-z.Peer-Reviewed Original ResearchMeSH KeywordsActivinsAnimalsCell Culture TechniquesCell DifferentiationDiabetes Mellitus, ExperimentalEmbryonic Stem CellsGene Expression RegulationHomeodomain ProteinsHumansMaleMiceMice, Inbred BALB CMice, NudePancreasReverse Transcriptase Polymerase Chain ReactionStem Cell TransplantationTrans-ActivatorsTransplantation, HeterologousTretinoinConceptsHuman embryonic stem cellsPdx1-positive cellsForkhead box protein A2Embryonic stem cellsEndodermal cellsCaudal-type homeobox transcription factor 2Differentiation of hESCsCell typesPancreatic cell fateRetinoic acidStem cellsMature cell typesEmbryonic germ layersEmbryoid body formationPancreatic cell typesBox protein A2Transcription factor 2Vivo pancreatic developmentEpigenetic signalsCell fateEctodermal differentiationDuodenal homeobox 1Pancreatic fateGene 17Gene expression