2019
Investigating the role of Osiris genes in Drosophila sechellia larval resistance to a host plant toxin
Lanno S, Shimshak S, Peyser R, Linde S, Coolon J. Investigating the role of Osiris genes in Drosophila sechellia larval resistance to a host plant toxin. Ecology And Evolution 2019, 9: 1922-1933. PMID: 30847082, PMCID: PMC6392368, DOI: 10.1002/ece3.4885.Peer-Reviewed Original ResearchOA resistanceAdaptive phenotypic changesResistant to OAGenomic regionsGenetic basisGenome-wide differential gene expressionPhenotypic changesToxic host plantsFine-mapping QTLDifferential gene expressionHost plant toxinsMultiple life stagesOsiris genesLife stagesGenetic mapHost specializationFamily genesHost plantsResistance genesRNA sequencingRNA interferenceExposure to OAGene expressionGenesFruit fly
2017
Transcriptomic Analysis of Octanoic Acid Response in Drosophila sechellia Using RNA-Sequencing
Lanno S, Gregory S, Shimshak S, Alverson M, Chiu K, Feil A, Findley M, Forman T, Gordon J, Ho J, Krupp J, Lam I, Lane J, Linde S, Morse A, Rusk S, Ryan R, Saniee A, Sheth R, Siranosian J, Sirichantaropart L, Sternlieb S, Zaccardi C, Coolon J. Transcriptomic Analysis of Octanoic Acid Response in Drosophila sechellia Using RNA-Sequencing. G3: Genes, Genomes, Genetics 2017, 7: 3867-3873. PMID: 29021218, PMCID: PMC5714484, DOI: 10.1534/g3.117.300297.Peer-Reviewed Original ResearchConceptsQuantitative trait lociOA resistanceGene expression analysisGene OntologyResistance genesRNA sequencingRNA interferenceExpression analysisConsistent with previous functional studiesFunctional studiesGene family membersAnnotated orthologsDrosophila sechelliaEffect lociRNA-seqLevels of octanoic acidTrait lociCuticle developmentDefense responsesExpressed genesToxic fruitsTranscriptome analysisDownregulated genesUpregulated genesGenesGenetic basis of octanoic acid resistance in Drosophila sechellia: functional analysis of a fine‐mapped region
López J, Lanno S, Auerbach J, Moskowitz E, Sligar L, Wittkopp P, Coolon J. Genetic basis of octanoic acid resistance in Drosophila sechellia: functional analysis of a fine‐mapped region. Molecular Ecology 2017, 26: 1148-1160. PMID: 28035709, PMCID: PMC5330365, DOI: 10.1111/mec.14001.Peer-Reviewed Original ResearchConceptsD. sechelliaOA resistanceDrosophila sechelliaRNA interferenceGenetic basisEvolutionary changesTissue-specific RNA interferenceFine-mapped regionKnock down expressionGene expression analysisD. simulansSister speciesChromosome 3RGenomic regionsD. melanogasterDefence compoundsLevel of expressionToxic fruitsFat bodySpecies of fruit fliesEnvironmental plasticityGenesSeychelles IslandsFruit flyFunctional analysis