2024
Comparative study of functional and structural muscle changes in peripheral artery disease: rubidium-82 positron emission tomography and histological correlation
Alashi A, Vermillion B, Callegari S, Burns R, Guo L, Moulton E, Guerrera N, Depino A, Papademetris X, Zeiss C, Thorn S, Liu C, Sinusas A. Comparative study of functional and structural muscle changes in peripheral artery disease: rubidium-82 positron emission tomography and histological correlation. European Heart Journal - Cardiovascular Imaging 2024, 25: jeae142.087. DOI: 10.1093/ehjci/jeae142.087.Peer-Reviewed Original ResearchPeripheral arterial diseaseStandardized uptake valueHindlimb ischemia modelReactive hyperemiaSkeletal muscle perfusionPerfusion reserveCapillary densityPET imagingArtery diseaseNon-ischemicRubidium-82 positron emission tomographyType 2 muscle fibersRelevant pre-clinical modelIndicative of fibrosisManagement of peripheral arterial diseaseCapillary to muscle fiber ratioClinically relevant pre-clinical modelPre-clinical modelsMuscle perfusionFast myosinWeeks post-ligationRb-82 uptakeEvaluate treatment strategiesRabbit hindlimb ischemia modelPositron emission tomography
2016
Effects of an endothelin receptor antagonist, Macitentan, on right ventricular substrate utilization and function in a Sugen 5416/hypoxia rat model of severe pulmonary arterial hypertension
Drozd K, Ahmadi A, Deng Y, Jiang B, Petryk J, Thorn S, Stewart D, Beanlands R, deKemp RA, DaSilva JN, Mielniczuk LM. Effects of an endothelin receptor antagonist, Macitentan, on right ventricular substrate utilization and function in a Sugen 5416/hypoxia rat model of severe pulmonary arterial hypertension. Journal Of Nuclear Cardiology 2016, 24: 1979-1989. PMID: 27688036, DOI: 10.1007/s12350-016-0663-4.Peer-Reviewed Original ResearchConceptsPulmonary arterial hypertensionRV FDG uptakePositron emission tomographyFDG uptakeFTHA uptakeFatty acid metabolismArterial hypertensionRV functionMacitentan treatmentRat modelSevere pulmonary arterial hypertensionWeek 5Effect of macitentanImproved RV functionRV systolic pressureWeeks of hypoxiaEndothelin receptor antagonistsRV ejection fractionHypoxia rat modelSingle subcutaneous injectionMale Sprague-DawleyAcid metabolismMyocardial energy metabolismPAH severitySugen 5416
2015
Insulin therapy normalizes reduced myocardial β-adrenoceptors at both the onset and after sustained hyperglycemia in diabetic rats
Haley JM, Thackeray JT, Kolajova M, Thorn SL, DaSilva JN. Insulin therapy normalizes reduced myocardial β-adrenoceptors at both the onset and after sustained hyperglycemia in diabetic rats. Life Sciences 2015, 132: 101-107. PMID: 25934520, DOI: 10.1016/j.lfs.2015.03.024.Peer-Reviewed Original ResearchConceptsCardiac β-ARPositron emission tomographyΒ-ARHeart rateInsulin therapyDiabetes mellitusΒ-adrenoceptorsCV functionWestern blottingInsulin effectDuration of hyperglycemiaMyocardial β-adrenoceptorsΒ-AR expressionOnset of hyperglycemiaWeeks of hyperglycemiaCardiac β-adrenoceptorsΒ-AR subtypesReduced heart rateMyocardial β-ARsGlycemic therapyStreptozotocin ratsSerial echocardiographySTZ ratsGlycemic controlCardiovascular dysfunctionPET imaging of a collagen matrix reveals its effective injection and targeted retention in a mouse model of myocardial infarction
Ahmadi A, Thorn SL, Alarcon EI, Kordos M, Padavan DT, Hadizad T, Cron GO, Beanlands RS, DaSilva JN, Ruel M, deKemp RA, Suuronen EJ. PET imaging of a collagen matrix reveals its effective injection and targeted retention in a mouse model of myocardial infarction. Biomaterials 2015, 49: 18-26. PMID: 25725551, DOI: 10.1016/j.biomaterials.2015.01.016.Peer-Reviewed Original ResearchConceptsMyocardial infarctionPositron emission tomographyPET imagingMouse modelNon-invasive PET imagingCardiac regeneration therapyIschemic territoryPET resultsInfarcted myocardiumEmission tomographyCollagen matrixMyocardial injectionEarly retentionPromising modalityRegeneration therapyInfarctionLabeling efficiencyMyocardiumFluorescence imagingImagingBiodistributionInjectionQdot labelingEx
2013
Chronic AMPK activity dysregulation produces myocardial insulin resistance in the human Arg302Gln-PRKAG2 glycogen storage disease mouse model
Thorn SL, Gollob MH, Harper ME, Beanlands RS, deKemp RA, DaSilva JN. Chronic AMPK activity dysregulation produces myocardial insulin resistance in the human Arg302Gln-PRKAG2 glycogen storage disease mouse model. EJNMMI Research 2013, 3: 48. PMID: 23829931, PMCID: PMC3707764, DOI: 10.1186/2191-219x-3-48.Peer-Reviewed Original ResearchMyocardial glucose uptakeCardiac glycogen storesSystolic functionInsulin resistanceAcute insulinMouse modelGlycogen storesDeoxyglucose positron emission tomographyProgressive conduction system diseaseGlucose uptakeSkeletal muscle glucose uptakeExcessive glycogen depositionDisease mouse modelMyocardial insulin resistanceMuscle glucose uptakeConduction system diseaseInsulin receptor expressionPositron emission tomographyPRKAG2 cardiac syndromeFrequent arrhythmiaInsulin stimulationMetabolic therapyReceptor expressionCardiac syndromeSystem diseases
2012
18F-FDG Cell Labeling May Underestimate Transplanted Cell Homing: More Accurate, Efficient, and Stable Cell Labeling with Hexadecyl-4-[18F]Fluorobenzoate for in Vivo Tracking of Transplanted Human Progenitor Cells by Positron Emission Tomography
Zhang Y, Dasilva JN, Hadizad T, Thorn S, Kuraitis D, Renaud JM, Ahmadi A, Kordos M, Dekemp RA, Beanlands RS, Suuronen EJ, Ruel M. 18F-FDG Cell Labeling May Underestimate Transplanted Cell Homing: More Accurate, Efficient, and Stable Cell Labeling with Hexadecyl-4-[18F]Fluorobenzoate for in Vivo Tracking of Transplanted Human Progenitor Cells by Positron Emission Tomography. Cell Transplantation 2012, 21: 1821-1835. PMID: 22469629, DOI: 10.3727/096368911x637416.Peer-Reviewed Original ResearchConceptsCirculating Progenitor CellsPositron emission tomographyEmission tomographyCell therapyPET imagingCell transplantation groupProgenitor cellsCoronary artery ligationRat myocardial infarction modelInfarct border zoneHeart tissue sectionsMyocardial infarction modelBorder zoneCardiac cell therapyTransplantation groupIschemic/Artery ligationH posttransplantationBest modalityHuman progenitor cellsIntramyocardial injectionH postinjectionInjection siteTissue biodistributionInfarction model
2011
Reduced CGP12177 binding to cardiac β-adrenoceptors in hyperglycemic high-fat-diet-fed, streptozotocin-induced diabetic rats
Thackeray JT, Parsa-Nezhad M, Kenk M, Thorn SL, Kolajova M, Beanlands RS, DaSilva JN. Reduced CGP12177 binding to cardiac β-adrenoceptors in hyperglycemic high-fat-diet-fed, streptozotocin-induced diabetic rats. Nuclear Medicine And Biology 2011, 38: 1059-1066. PMID: 21831645, DOI: 10.1016/j.nucmedbio.2011.04.002.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic beta-AntagonistsAnimalsBiomarkersBlotting, WesternDiabetes Mellitus, ExperimentalDiet, High-FatGene Expression RegulationHyperglycemiaMaleMyocardiumPositron-Emission TomographyPropanolaminesProtein BindingRatsRats, Sprague-DawleyReceptors, Adrenergic, beta-1Receptors, Adrenergic, beta-2Substrate SpecificityConceptsAbnormal sympathetic nervous systemΒ-AR expressionSympathetic nervous systemDiabetic rat heartsSingle intraperitoneal injectionVehicle-treated controlsInfusion of isoproterenolCardiac β-adrenoceptorsΒ-AR subtypesΒ-AR antagonistsSpecific bindingHalf of ratsNormal Sprague-DawleyΒ-AR stimulationPositron emission tomographyΒ-adrenoceptor signalingCardiac bindingAR expressionCardiac dysfunctionDiabetic ratsInsulin resistanceSustained hyperglycemiaIntraperitoneal injectionΒ-adrenoceptorsDiet feeding
2010
Alterations of pre- and postsynaptic noradrenergic signaling in a rat model of adriamycin-induced cardiotoxicity
Kenk M, Thackeray JT, Thorn SL, Dhami K, Chow BJ, Ascah KJ, DaSilva JN, Beanlands RS. Alterations of pre- and postsynaptic noradrenergic signaling in a rat model of adriamycin-induced cardiotoxicity. Journal Of Nuclear Cardiology 2010, 17: 254-263. PMID: 20182926, DOI: 10.1007/s12350-009-9190-x.Peer-Reviewed Original ResearchConceptsPositron emission tomographyRat modelNoradrenergic signalingHeart/body weight ratioBeta-adrenergic receptor antagonistMyocardial noradrenaline levelsSympathetic nervous systemBody weight ratioPhosphodiesterase 4 inhibitorBeta-adrenergic receptorsVentricle free wallAnthracycline chemotherapeutic agentDesipramine treatmentNoradrenaline levelsNoradrenaline uptakeAnthracycline cardiotoxicityReceptor antagonistAcute increaseCardiac functionRight ventricleLeft atriumInteraction of preAdriamycin cardiotoxicityFree wallNervous system
2009
In Vivo Assessment of Myocardial Glucose Uptake by Positron Emission Tomography in Adults With the PRKAG2 Cardiac Syndrome
Ha AC, Renaud JM, deKemp RA, Thorn S, DaSilva J, Garrard L, Yoshinaga K, Abraham A, Green MS, Beanlands RS, Gollob MH. In Vivo Assessment of Myocardial Glucose Uptake by Positron Emission Tomography in Adults With the PRKAG2 Cardiac Syndrome. Circulation Cardiovascular Imaging 2009, 2: 485-491. PMID: 19920047, DOI: 10.1161/circimaging.109.853291.Peer-Reviewed Original ResearchMeSH KeywordsAdultAMP-Activated Protein KinasesCase-Control StudiesEchocardiographyFemaleFluorodeoxyglucose F18Heart DiseasesHumansMaleMetabolic DiseasesMiddle AgedMutationMyocardiumPhenotypeRadiographic Image Interpretation, Computer-AssistedRubidium RadioisotopesStatistics, NonparametricSyndromeTomography, Emission-ComputedConceptsMyocardial glucose uptakeSignificant myocardial scarFDG-PET imagingPRKAG2 cardiac syndromeCardiac syndromeGlucose uptakeAdult patientsMyocardial scarMetabolic diseasesControl groupMedian blood glucoseEuglycemic hyperinsulinemic clampStable blood glucose levelsBlood glucose levelsInherited metabolic diseaseMin/Positron emission tomography (PET) imagingEmission Tomography ImagingPositron emission tomographyMyocardial glucose transportPerfusion scanBlood glucoseControl subjectsHyperinsulinemic clampAffected patients
2008
Collagen-Based Matrices Improve the Delivery of Transplanted Circulating Progenitor Cells
Zhang Y, Thorn S, DaSilva JN, Lamoureux M, deKemp RA, Beanlands RS, Ruel M, Suuronen EJ. Collagen-Based Matrices Improve the Delivery of Transplanted Circulating Progenitor Cells. Circulation Cardiovascular Imaging 2008, 1: 197-204. PMID: 19808543, DOI: 10.1161/circimaging.108.781120.Peer-Reviewed Original ResearchConceptsPositron emission tomographyIschemic hind limbIschemic hind limb musclesCirculating Progenitor CellsHind limbProgenitor cellsRats 2 weeksFemoral artery ligationNonspecific tissueHind limb musclesMechanism of actionEarly posttransplantationCollagen matrixSmall animal positron emission tomographyArtery ligationMinutes postinjectionProgenitor cell retentionAnimal positron emission tomographyLimb musclesCell therapyHuman CPCsTarget tissuesPosttransplantationTomographyFDG
2005
Application of cardiac molecular imaging using positron emission tomography in evaluation of drug and therapeutics for cardiovascular disorders.
Yoshinaga K, Chow BJ, dekemp RA, Thorn S, Ruddy TD, Davies RA, DaSilva JN, Beanlands R. Application of cardiac molecular imaging using positron emission tomography in evaluation of drug and therapeutics for cardiovascular disorders. Current Pharmaceutical Design 2005, 11: 903-32. PMID: 15777243, DOI: 10.2174/1381612053381800.Peer-Reviewed Original ResearchConceptsVivo pharmacokinetic studyDrug deliveryMolecular probesCardiovascular diseaseEffect of therapyAccuracy of PETPET/CTNon-invasive imaging modalityEvaluation of drugsMolecular imagingPositron emission tomography (PET) imagingEmission Tomography ImagingPositron emission tomographyPotential of PETNon-invasive modalityMechanism of actionPharmacokinetic studyCardiac molecular imagingEarly drug developmentAdvantages of PETDrug therapyDisease progressionSerial evaluationDrug treatmentReceptor levels