2023
Activity‐based Tools for Interrogating Host Biology During Infection
Ramanathan R, Hatzios S. Activity‐based Tools for Interrogating Host Biology During Infection. Israel Journal Of Chemistry 2023, 63 PMID: 37744997, PMCID: PMC10512441, DOI: 10.1002/ijch.202200095.Peer-Reviewed Original ResearchActivity-based protein profilingPost-translational modificationsHost post-translational modificationsHost-microbe interactionsHost biologyCells senseCell signalingMicrobial mechanismsEnzyme functionProtein profilingProtein structureSide-chain reactivityChemical probesInfected cellsHost-directed therapiesPotential targetRapid modulationHost responseSignalingBiologyInfectionEnzymeProfilingPathwayPathogens
2019
Functional characterization of a subtilisin-like serine protease from Vibrio cholerae
Howell M, Dumitrescu DG, Blankenship LR, Herkert D, Hatzios SK. Functional characterization of a subtilisin-like serine protease from Vibrio cholerae. Journal Of Biological Chemistry 2019, 294: 9888-9900. PMID: 31076508, PMCID: PMC6597830, DOI: 10.1074/jbc.ra119.007745.Peer-Reviewed Original ResearchConceptsSubtilisin-like enzymesSerine proteasesSubtilisin-like serine proteaseProtein domain structureDiarrheal disease choleraSerine hydrolase activityNutrient acquisitionAquatic nichesCarbohydrate-binding proteinsPathogen enzymesMature enzymeReporter proteinBiofilm assemblyFunctional characterizationNumerous enzymesDisease choleraCleavage eventsIntelectinLarge familyStepwise processingVibrio choleraeCleavage patternsEnzymeProteaseHydrolase activity
2018
Activity-Based Protein Profiling at the Host–Pathogen Interface
Kovalyova Y, Hatzios SK. Activity-Based Protein Profiling at the Host–Pathogen Interface. Current Topics In Microbiology And Immunology 2018, 420: 73-91. PMID: 30203396, DOI: 10.1007/82_2018_129.BooksConceptsActivity-based protein profilingHost-pathogen interfaceProtein profilingFunctional proteomePathogen interactionsMicrobial pathogenicityComplex proteomesEnzyme-mediated mechanismReactive amino acidsActive enzymeChemical probesAmino acidsMetabolic adaptationProteomeMicrobial infectionsCo-culture systemBiological systemsHost immunityEnzymeProfilingPathogenicityHostProbeAnimal modelsAdaptation