Featured Publications
Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function
Gorenberg EL, Tieze S, Yücel B, Zhao HR, Chou V, Wirak GS, Tomita S, Lam TT, Chandra SS. Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function. PLOS Biology 2022, 20: e3001590. PMID: 35358180, PMCID: PMC9004782, DOI: 10.1371/journal.pbio.3001590.Peer-Reviewed Original ResearchConceptsPalmitoyl-protein thioesterase 1Disulfide bond formationNeuronal ceroid lipofuscinosisPosttranslational modificationsRole of PPT1Identification of substratesResin-assisted captureEnzyme palmitoyl-protein thioesterase 1Synaptic adhesion moleculesNeurodegenerative diseasesMature proteinMitochondrial proteinsMolecular dissectionPutative substratesDepalmitoylationKnockout mouse brainFunction mutationsStringent screenMolecular pathwaysSynapse functionDisulfide bondsProteinDevastating neurodegenerative diseaseDisease mechanismsSynaptic function
2023
Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration
Tejwani L, Jung Y, Kokubu H, Sowmithra S, Ni L, Lee C, Sanders B, Lee P, Xiang Y, Luttik K, Soriano A, Yoon J, Park J, Ro H, Ju H, Liao C, Tieze S, Rigo F, Jafar-Nejad P, Lim J. Reduction of Nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration. Journal Of Clinical Investigation 2023, 133: e138207. PMID: 37384409, PMCID: PMC10425213, DOI: 10.1172/jci138207.Peer-Reviewed Original ResearchConceptsAmyotrophic lateral sclerosisTDP-43-related neurodegenerationNeurodegenerative disordersTransactive response DNA-binding protein 43Sporadic amyotrophic lateral sclerosisDNA-binding protein 43Subset of patientsTDP-43 speciesTDP-43 inclusionsDistinct mouse modelsTDP-43 proteinopathyFamilial amyotrophic lateral sclerosisNemo-like kinaseMultiple neurodegenerative disordersAutophagy/lysosome pathwayTDP-43-positive aggregatesALS patientsALS casesSporadic ALSPharmacological reductionProtein 43Lateral sclerosisMouse modelParkinson's diseaseTDP-43BioSentinel: Validating Sensitivity of Yeast Biosensors to Deep Space Relevant Radiation
Liddell L, Gentry D, Gilbert R, Marina D, Tieze S, Padgen M, Akiyama K, Keenan K, Bhattacharya S, Santa Maria S. BioSentinel: Validating Sensitivity of Yeast Biosensors to Deep Space Relevant Radiation. Astrobiology 2023, 23: 648-656. PMID: 37052477, PMCID: PMC10254971, DOI: 10.1089/ast.2022.0124.Peer-Reviewed Original Research
2022
Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain.
Massaro Tieze S, Chandra SS, Vidyadhara DJ. Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain. Journal Of Visualized Experiments 2022 PMID: 36190269, PMCID: PMC10936730, DOI: 10.3791/64574.Peer-Reviewed Original ResearchConceptsSubcellular fractionationPost-synaptic compartmentsBiochemical isolationSub-synaptic compartmentsSynaptic cytosolPlasma membraneMolecular basisEnrichment analysisNeuronal preMolecular etiologySynaptic vesiclesSynaptic terminalsSynaptic plasma membranesSynaptic componentsBrain tissue homogenizationSynaptic terminal structureTerminal structureActive stateNeuronal communicationMurine brainSynaptic structureFunctional characteristicsMouse brainHypotonic lysisSynaptic dysfunction
2020
BioSentinel: A Biological CubeSat for Deep Space Exploration
Tieze S, Liddell LC, Santa Maria SR, Bhattacharya S. BioSentinel: A Biological CubeSat for Deep Space Exploration. Astrobiology 2020, 23: 631-636. PMID: 32282239, PMCID: PMC10254969, DOI: 10.1089/ast.2019.2068.Peer-Reviewed Original ResearchBioSentinel: Long-Term Saccharomyces cerevisiae Preservation for a Deep Space Biosensor Mission
Santa Maria SR, Marina DB, Tieze S, Liddell LC, Bhattacharya S. BioSentinel: Long-Term Saccharomyces cerevisiae Preservation for a Deep Space Biosensor Mission. Astrobiology 2020, 23: 617-630. PMID: 31905002, PMCID: PMC10254977, DOI: 10.1089/ast.2019.2073.Peer-Reviewed Original Research