2014
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
Wu X, Scott DA, Kriz AJ, Chiu AC, Hsu PD, Dadon DB, Cheng AW, Trevino AE, Konermann S, Chen S, Jaenisch R, Zhang F, Sharp PA. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nature Biotechnology 2014, 32: 670-676. PMID: 24752079, PMCID: PMC4145672, DOI: 10.1038/nbt.2889.Peer-Reviewed Original ResearchConceptsMammalian cellsGenome-wide bindingGenome-wide analysisWidespread bindingBindingCellsCRISPRLow levelsCleavage
2012
Adaptive Evolution and the Birth of CTCF Binding Sites in the Drosophila Genome
Ni X, Zhang YE, Nègre N, Chen S, Long M, White KP. Adaptive Evolution and the Birth of CTCF Binding Sites in the Drosophila Genome. PLOS Biology 2012, 10: e1001420. PMID: 23139640, PMCID: PMC3491045, DOI: 10.1371/journal.pbio.1001420.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, BiologicalAnimalsBase SequenceBinding SitesCCCTC-Binding FactorConserved SequenceDrosophilaDrosophila ProteinsEvolution, MolecularGenetic DriftGenetics, PopulationGenome, InsectOligonucleotide Array Sequence AnalysisPhylogenyProtein BindingRepressor ProteinsSelection, GeneticSpecies SpecificityTime FactorsConceptsCTCF binding siteDrosophila melanogasterNew genesCis-regulatory DNA sequencesGene expressionSpecies of DrosophilaGenome-wide bindingSignatures of selectionGenomes of humansComparative gene expression profilingGenome sequence dataRecent common ancestorGene expression profilingBinding sitesExpression divergenceGenome evolutionAdaptive evolutionDrosophila genomeDrosophila simulansEvolutionary ratesCTCF bindingCTCF proteinEvolutionary distanceNew CTCFCommon ancestor