Tumor Necrosis Factor-α and Interleukin-1β Regulate Interleukin-8 Expression in Third Trimester Decidual Cells Implications for the Genesis of Chorioamnionitis
Lockwood CJ, Arcuri F, Toti P, De Felice C, Krikun G, Guller S, Buchwalder LF, Schatz F. Tumor Necrosis Factor-α and Interleukin-1β Regulate Interleukin-8 Expression in Third Trimester Decidual Cells Implications for the Genesis of Chorioamnionitis. American Journal Of Pathology 2006, 169: 1294-1302. PMID: 17003486, PMCID: PMC1698845, DOI: 10.2353/ajpath.2006.060185.Peer-Reviewed Original ResearchConceptsTerm decidual cellsIL-8 expressionInterleukin-8 expressionTranscriptase-polymerase chain reactionDecidual cellsNeutrophil infiltrationIL-8TNF-alphaIL-8 secretion levelsIL-8 levelsIntense neutrophil infiltrationReverse transcriptase-polymerase chain reactionChain reactionInterleukin-1 betaTumor necrosis factorIL-8 mRNAEnzyme-linked immunosorbentIL-8 outputConcentration-response studiesNeutrophil infiltrateSteroidal milieuTerm deciduaTumor necrosisNecrosis factorImmunohistochemical stainingFirst-trimester trophoblast cell model gene response to hypoxia
Koklanaris N, Nwachukwu JC, Huang SJ, Guller S, Karpisheva K, Garabedian M, Lee MJ. First-trimester trophoblast cell model gene response to hypoxia. American Journal Of Obstetrics And Gynecology 2006, 194: 687-693. PMID: 16522398, DOI: 10.1016/j.ajog.2006.01.067.Peer-Reviewed Original ResearchConceptsReverse transcriptase-polymerase chain reactionQuantitative reverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionPolymerase chain reactionHypoxia-responsive genesHTR-8/SVneoChain reactionCell linesTrophoblast cell linePregnancy outcomesEarly pregnancyMotility/migrationHypoxic environmentTotal RNAHypoxiaPlacentationMicroarray studiesGene responsesPreeclampsiaPregnancy