2019
Differential gene expression during placentation in pregnancies conceived with different fertility treatments compared with spontaneous pregnancies
Lee B, Koeppel AF, Wang ET, Gonzalez TL, Sun T, Kroener L, Lin Y, Joshi NV, Ghadiali T, Turner SD, Rich SS, Farber CR, Rotter JI, Ida Chen YD, Goodarzi MO, Guller S, Harwood B, Serna TB, Williams J, Pisarska MD. Differential gene expression during placentation in pregnancies conceived with different fertility treatments compared with spontaneous pregnancies. Fertility And Sterility 2019, 111: 535-546. PMID: 30611556, PMCID: PMC7156023, DOI: 10.1016/j.fertnstert.2018.11.005.Peer-Reviewed Original ResearchConceptsFirst trimester placentaSpontaneous pregnancyIVF groupFertility treatmentMaternal medical conditionsTreatment-related factorsAcademic medical centerTranscriptomic profilesGestational age 11Fetal demographicsInfertility groupPregnancy complicationsCohort studySpontaneous conceptionMedical CenterMAIN OUTCOMEMedical conditionsPregnancyDifferent fertility treatmentsIVFLarger studyDifferential gene expressionChorionic villiGene expressionPlacenta
2016
Optimization of techniques for multiple platform testing in small, precious samples such as human chorionic villus sampling
Pisarska MD, Akhlaghpour M, Lee B, Barlow GM, Xu N, Wang ET, Mackey AJ, Farber CR, Rich SS, Rotter JI, Chen Y, Goodarzi MO, Guller S, Williams J. Optimization of techniques for multiple platform testing in small, precious samples such as human chorionic villus sampling. Prenatal Diagnosis 2016, 36: 1061-1070. PMID: 27718505, PMCID: PMC5142835, DOI: 10.1002/pd.4936.Peer-Reviewed Original ResearchConceptsGenome-wide methylation profilingRNA-seq librariesSystems biology approachGene expression studiesBiology approachEpigenetic modificationsLow DNA yieldRNA sequencingMethylation profilingExpression studiesGene expressionPurification of DNAQuality DNAGlobal changeHighest RNA yieldDNA yieldRNA yieldRNADNAIsolation kitRNAlater samplesRNAlaterGenesChorionic villiMultiple testing