Site-Specific Cleavage of Human Chromosome 4 Mediated by Triple-Helix Formation
Strobel S, Doucette-Stamm L, Riba L, Housman D, Dervan P. Site-Specific Cleavage of Human Chromosome 4 Mediated by Triple-Helix Formation. Science 1991, 254: 1639-1642. PMID: 1836279, DOI: 10.1126/science.1836279.Peer-Reviewed Original ResearchConceptsHuman chromosome 4Human chromosomesChromosome 4Disease genesTriple helix formationEntire candidate regionSpecific DNA segmentsCandidate gene lociSite-specific cleavageGigabase pairsHuman genomeEnzymatic cleavageRecognition motifDNA segmentsGene locusGenomic DNAHuntington's disease geneHuman geneticsTarget siteChromosomesSized fragmentsCandidate regionsCleavageGenomePhysical isolationSingle-site enzymatic cleavage of yeast genomic DNA mediated by triple helix formation
Strobel S, Dervan P. Single-site enzymatic cleavage of yeast genomic DNA mediated by triple helix formation. Nature 1991, 350: 172-174. PMID: 1848684, DOI: 10.1038/350172a0.Peer-Reviewed Original ResearchConceptsTriple helix formationGenomic DNABase pairsYeast genomic DNAHelix formationSequence-specific DNALarge genomic DNAGenomic libraryMegabase pairsPhysical mappingEnzymatic cleavageRecognition motifSpecificity of bindingChromosomal DNARestriction endonuclease digestionManageable fragmentsRecognition sequenceGenetic markersDNA showRestriction enzymesQuantitative cleavageLarge DNADNAEndonuclease digestionChromosomes