2020
Dual role of reactive oxygen species in autophagy and apoptosis induced by compound PN in prostate cancer cells
Choi H, Kim K, Park K, Kim S, Park S, Yu S, Kim Y, Kim D, Chung K, Ahn S. Dual role of reactive oxygen species in autophagy and apoptosis induced by compound PN in prostate cancer cells. Molecular & Cellular Toxicology 2020, 17: 41-50. DOI: 10.1007/s13273-020-00107-4.Peer-Reviewed Original ResearchMitochondrial reactive oxygen speciesReactive oxygen speciesCell deathMolecular mechanismsProstate cancer cellsPC-3 cellsCell survivalAnti-cancer activityROS productionApoptotic cell deathCancer cellsOxygen speciesROS-dependent mannerInduction of apoptosisProstate cancer PC-3 cellsHuman prostate cancer cellsAcceleration of apoptosisCancer PC-3 cellsInhibition of autophagyProduction of intracellularIntracellular ROS scavengerProstate cancer treatmentExpression of apoptosisAutophagy inductionWestern blot analysis
2017
Salinomycin induces endoplasmic reticulum stress-mediated autophagy and apoptosis through generation of reactive oxygen species in human glioma U87MG cells
Yu S, Kim S, Kim K, Ji J, Seo Y, Yu H, Ahn S. Salinomycin induces endoplasmic reticulum stress-mediated autophagy and apoptosis through generation of reactive oxygen species in human glioma U87MG cells. Oncology Reports 2017, 37: 3321-3328. PMID: 28498472, DOI: 10.3892/or.2017.5615.Peer-Reviewed Original ResearchConceptsReactive oxygen speciesER stressHuman glioma U87MG cellsEndoplasmic reticulumGlioma U87MG cellsU87MG cellsStress-related proteinsAcidic vesicular organellesOxygen speciesER stress-related proteinsHuman cancer cellsAutophagy marker proteinsVesicular organellesImpact of salinomycinEndoplasmic reticulum stress-mediated autophagyMolecular mechanismsCell deathAutophagyMarker proteinsAutophagy inhibitorCancer cell linesGlioma cancer cell linesInduced apoptosisROS scavengerExpression levelsDeoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells
Kim S, Son K, Kim K, Yu S, Park S, Kim Y, Nam H, Suh J, Ji J, Ahn S. Deoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells. Pharmacological Reports 2017, 69: 878-884. PMID: 28623712, DOI: 10.1016/j.pharep.2017.04.007.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic Agents, PhytogenicApoptosisAutophagyBone NeoplasmsCell Line, TumorCell SurvivalDrugs, Chinese HerbalGene Expression RegulationHumansOsteosarcomaPhosphoinositide-3 Kinase InhibitorsPodophyllotoxinProto-Oncogene Proteins c-aktReactive Oxygen SpeciesTOR Serine-Threonine KinasesConceptsReactive oxygen speciesPI3K/AKT/mTOR pathwayAKT/mTOR pathwayU2OS cellsAcidic vesicular organelles (AVOs) formationMTOR pathwayMitochondrial reactive oxygen speciesOsteosarcoma U2OS cellsCell death modeOxygen speciesPropidium iodidePI3K/Akt/mTORCell cycle arrestLight chain 3Cell viabilityCellular processesInhibition of apoptosisAkt/mTOROrganelle formationAcridine orange stainingAutophagy processApoptotic responseDeath modeWestern blot analysisCell survivalSalinomycin Induces Reactive Oxygen Species and Apoptosis in Aggressive Breast Cancer Cells as Mediated with Regulation of Autophagy
Kim K, Park K, Kim S, Yu S, Lee D, Kim Y, Noh K, YEUL J, Seo Y, Ahn S. Salinomycin Induces Reactive Oxygen Species and Apoptosis in Aggressive Breast Cancer Cells as Mediated with Regulation of Autophagy. Anticancer Research 2017, 37: 1747-1758. PMID: 28373437, DOI: 10.21873/anticanres.11507.Peer-Reviewed Original ResearchConceptsMDA-MB-231 cellsAggressive breast cancer cellsBreast cancer cellsCancer cellsReactive oxygen speciesBreast cancer cell linesROS productionMitochondrial membrane potentialScavenger of ROSInduces reactive oxygen speciesCancer cell linesCaspase-3/9 activityPropidium iodide stainingMCF-7 cellsOxygen speciesAcceleration of apoptosisAcridine orange stainingAutophagy inhibitionMonocarboxylic ionophoreChemotherapeutic drugsCancer treatmentMitochondrial dysfunctionIodide stainingRegulation of autophagyUnderlying mechanism
2016
Salinomycin simultaneously induces apoptosis and autophagy through generation of reactive oxygen species in osteosarcoma U2OS cells
Kim S, Choi Y, Kim K, Yu S, Seo Y, Chun S, Noh K, Suh J, Ahn S. Salinomycin simultaneously induces apoptosis and autophagy through generation of reactive oxygen species in osteosarcoma U2OS cells. Biochemical And Biophysical Research Communications 2016, 473: 607-613. PMID: 27033598, DOI: 10.1016/j.bbrc.2016.03.132.Peer-Reviewed Original ResearchConceptsAcidic vesicular organellesReactive oxygen speciesMitochondrial membrane potentialChange of MMPOsteosarcoma U2OS cellsInhibition of autophagyU2OS cellsExpression of apoptosisCancer cell linesOxygen speciesFlow cytometryWestern blottingCaspase-3Cancer therapyVesicular organellesPoly (ADP-ribose) polymerase
2013
Interplay of reactive oxygen species, intracellular Ca2+ and mitochondrial homeostasis in the apoptosis of prostate cancer cells by deoxypodophyllotoxin
Kim K, Cho H, Yu S, Kim S, Yu H, Park Y, Mirkheshti N, Kim S, Song C, Chatterjee B, Ahn S. Interplay of reactive oxygen species, intracellular Ca2+ and mitochondrial homeostasis in the apoptosis of prostate cancer cells by deoxypodophyllotoxin. Journal Of Cellular Biochemistry 2013, 114: 1124-1134. PMID: 23192945, DOI: 10.1002/jcb.24455.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisBcl-2-Associated X ProteinCalciumCaspase 3Cell Line, TumorCell ProliferationCell SurvivalCytochromes cDrug Screening Assays, AntitumorDrugs, Chinese HerbalEgtazic AcidEnzyme ActivationG2 Phase Cell Cycle CheckpointsHomeostasisHumansIntracellular SpaceM Phase Cell Cycle CheckpointsMaleMembrane Potential, MitochondrialMitochondriaModels, BiologicalPodophyllotoxinProstatic NeoplasmsProtein TransportReactive Oxygen SpeciesConceptsReactive oxygen speciesMitochondrial homeostasisProstate cancer cellsCaspase-3-dependent pathwayCytochrome c releasePC-3 cell modelBax protein translocationTurn induced apoptosisCancer cellsMitochondrial membrane potentialOxygen speciesCaspase-3 activationN-acetylcysteineIncrease of ROSProtein translocationProstate cancerHuman prostate cancer cellsGeneration of ROSC releaseAntioxidant N-acetylcysteineNew anti-neoplastic agentsROS accumulationMitochondrial functionRecurrent prostate cancerLimited treatment options