1999
The Acidic Region and Conserved Putative Protein Kinase C Phosphorylation Site in Nef Are Important for SIV Replication in Rhesus Macaques
Carl S, Iafrate A, Lang S, Stahl-Hennig C, Kuhn E, Fuchs D, Mätz-Rensing K, Haaft P, Heeney J, Skowronski J, Kirchhoff F. The Acidic Region and Conserved Putative Protein Kinase C Phosphorylation Site in Nef Are Important for SIV Replication in Rhesus Macaques. Virology 1999, 257: 138-155. PMID: 10208928, DOI: 10.1006/viro.1999.9645.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCD4 AntigensCOS CellsGene Products, nefHistocompatibility Antigens Class IHIV-1Hydrogen-Ion ConcentrationMacaca mulattaMolecular Sequence DataMutagenesis, Site-DirectedNef Gene Products, Human Immunodeficiency VirusPhosphorylationProtein Kinase CSimian immunodeficiency virusSurface PropertiesVirus ReplicationConceptsProtein kinase C phosphorylation sitesKinase C phosphorylation sitesC phosphorylation sitesPhosphorylation sitesPutative protein kinase C phosphorylation sitePotential protein kinase C phosphorylation sitesAcidic regionWild-type activityPKC phosphorylation sitesJurkat T cellsSignal transductionAbility of NefReplication assaysFunctional relevanceCell culture systemMutant virusNef functionPathogenic SIVmac239 cloneAcidic chargeReduced infectivityReplicationCulture systemNefRapid reversionRhesus macaques
1995
Identification of Lck-binding Elements in Tip of Herpesvirus Saimiri (∗)
Jung J, Lang S, Friedrich U, Jun T, Roberts T, Desrosiers R, Biesinger B. Identification of Lck-binding Elements in Tip of Herpesvirus Saimiri (∗). Journal Of Biological Chemistry 1995, 270: 20660-20667. PMID: 7544793, DOI: 10.1074/jbc.270.35.20660.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCell LineChlorocebus aethiopsDNA PrimersHerpesvirus 2, SaimiriineLymphocyte Specific Protein Tyrosine Kinase p56(lck)Molecular Sequence DataMutagenesis, Site-DirectedPeptide FragmentsPhosphoproteinsPhosphoserinePhosphothreoninePhosphotyrosinePoint MutationPolymerase Chain ReactionProtein-Tyrosine KinasesRatsRecombinant ProteinsSequence Homology, Amino AcidSpodopteraStructure-Activity RelationshipTransfectionTyrosineViral ProteinsConceptsC-terminal SrcCellular protein kinasesBinding of sequencesInteraction of TipHerpesvirus saimiriLck kinase activityCOS-1 cellsRat-1 cell lineLck functionPutative SH3Cellular proteinsSH3 bindingProtein kinaseCarboxyl terminusLck kinaseVivo associationKinase activityMutational analysisFusion proteinLckAmino acidsKinaseHuman T cellsProteinCell lines