2006
A Genetic System for Rhesus Monkey Rhadinovirus: Use of Recombinant Virus To Quantitate Antibody-Mediated Neutralization
Bilello J, Morgan J, Damania B, Lang S, Desrosiers R. A Genetic System for Rhesus Monkey Rhadinovirus: Use of Recombinant Virus To Quantitate Antibody-Mediated Neutralization. Journal Of Virology 2006, 80: 1549-1562. PMID: 16415030, PMCID: PMC1346924, DOI: 10.1128/jvi.80.3.1549-1562.2006.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsAntibodies, ViralBase SequenceCell LineCloning, MolecularCosmidsDNA, ViralGene LibraryGenes, ReporterGenome, ViralGreen Fluorescent ProteinsHerpesviridae InfectionsHumansMacaca mulattaNeutralization TestsRecombination, GeneticRhadinovirusTumor Virus InfectionsConceptsRhesus monkey rhadinovirusGreen fluorescent proteinGenetic systemRhesus monkey fibroblastsGFP reporter geneMonkey fibroblastsGamma-2 herpesvirusKaposi's sarcoma-associated herpesvirusDifferent cell typesGenome reconstitutionSarcoma-associated herpesvirusCosmid clonesTerminal repeat regionR1 geneReporter geneRepeat regionFluorescent proteinExpression cassetteCell typesSEAP activityRRV infectivityRecombinant virusesGenomeB cellsGenes
1995
Downregulation of Lck-mediated signal transduction by tip of herpesvirus saimiri
Jung J, Lang S, Jun T, Roberts T, Veillette A, Desrosiers R. Downregulation of Lck-mediated signal transduction by tip of herpesvirus saimiri. Journal Of Virology 1995, 69: 7814-7822. PMID: 7494293, PMCID: PMC189725, DOI: 10.1128/jvi.69.12.7814-7822.1995.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell LineCell Line, TransformedCloning, MolecularDNA PrimersElectrophoresis, Polyacrylamide GelGene ExpressionHerpesvirus 2, SaimiriineHumansLymphocyte Specific Protein Tyrosine Kinase p56(lck)Molecular Sequence DataMolecular WeightPhosphoproteinsPlasmidsPolymerase Chain ReactionRecombinant Fusion ProteinsSignal TransductionSrc-Family KinasesT-LymphocytesTumor Cells, CulturedViral ProteinsConceptsExpression of tipSignal transductionTyrosine phosphorylationT cell signal transductionCellular signal transductionCellular tyrosine phosphorylationHuman Jurkat T cell lineJurkat T cell lineJurkat T cellsInhibition of LckSH3 domainLckTransductionHerpesvirus saimiriFibroblast cellsPhosphorylationStable complexesSurface expressionBinding assaysComplex formationExpressionCellsAnti-CD3 stimulationT cellsB cellsIdentification of Lck-binding Elements in Tip of Herpesvirus Saimiri (∗)
Jung J, Lang S, Friedrich U, Jun T, Roberts T, Desrosiers R, Biesinger B. Identification of Lck-binding Elements in Tip of Herpesvirus Saimiri (∗). Journal Of Biological Chemistry 1995, 270: 20660-20667. PMID: 7544793, DOI: 10.1074/jbc.270.35.20660.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCell LineChlorocebus aethiopsDNA PrimersHerpesvirus 2, SaimiriineLymphocyte Specific Protein Tyrosine Kinase p56(lck)Molecular Sequence DataMutagenesis, Site-DirectedPeptide FragmentsPhosphoproteinsPhosphoserinePhosphothreoninePhosphotyrosinePoint MutationPolymerase Chain ReactionProtein-Tyrosine KinasesRatsRecombinant ProteinsSequence Homology, Amino AcidSpodopteraStructure-Activity RelationshipTransfectionTyrosineViral ProteinsConceptsC-terminal SrcCellular protein kinasesBinding of sequencesInteraction of TipHerpesvirus saimiriLck kinase activityCOS-1 cellsRat-1 cell lineLck functionPutative SH3Cellular proteinsSH3 bindingProtein kinaseCarboxyl terminusLck kinaseVivo associationKinase activityMutational analysisFusion proteinLckAmino acidsKinaseHuman T cellsProteinCell linesIdentification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys
Du Z, Lang S, Sasseville V, Lackner A, Ilyinskii P, Daniel M, Jung J, Desrosiers R. Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys. Cell 1995, 82: 665-674. PMID: 7664345, DOI: 10.1016/0092-8674(95)90038-1.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAllelesAmino Acid SequenceAnimalsBase SequenceDNA PrimersDNA, ViralGene Products, envGene Products, nefGenes, nefLymphocyte ActivationMacaca mulattaMiceMolecular Sequence DataPhagocytesPhenotypePhosphorylationRetroviridae Proteins, OncogenicSignal TransductionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusTransformation, GeneticTyrosineViral Fusion ProteinsVirus ReplicationConceptsAcute diseasePeripheral blood mononuclear cell culturesBlood mononuclear cell culturesMononuclear cell culturesT lymphocyte activationSevere diarrheaLymphoid proliferationsGastrointestinal tractNef allelesMacaque monkeysLymphocyte activationCellular activationSIVmac239NefDiseaseActivationMonkeysCell culturesImportant determinantCellsNIH 3T3 cellsTyrosine phosphorylationCotransfected COS cellsCOS cellsDiarrhea
1993
Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus
Lang S, Weeger M, Stahl-Hennig C, Coulibaly C, Hunsmann G, Müller J, Müller-Hermelink H, Fuchs D, Wachter H, Daniel M. Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus. Journal Of Virology 1993, 67: 902-912. PMID: 8380472, PMCID: PMC237444, DOI: 10.1128/jvi.67.2.902-912.1993.Peer-Reviewed Original ResearchConceptsVpr variantsTAA stop codonVpr geneATG start codonSite-specific mutagenesisVpr mutant virusSignificant selective pressureImportance of VprStart codonSelective pressureVpr mutantsStop codonGenesPathogenic molecular cloneMolecular cloneCell linesVprTest animalsCEMx174 cell lineCodonNef geneSimilar kineticsVirus replicationReversionStop signal