Featured Publications
Dynamic Regulation of JAK-STAT Signaling Through the Prolactin Receptor Predicted by Computational Modeling
Mortlock RD, Georgia SK, Finley SD. Dynamic Regulation of JAK-STAT Signaling Through the Prolactin Receptor Predicted by Computational Modeling. Cellular And Molecular Bioengineering 2020, 14: 15-30. PMID: 33633812, PMCID: PMC7878662, DOI: 10.1007/s12195-020-00647-8.Peer-Reviewed Original ResearchBeta-cell massBeta cellsInsulin-producing beta cellsReceptor internalizationContext of pregnancyPrimary beta cellsJAK-STATPancreatic beta cellsBeta cell expansionBeta-cell survivalSTAT5 activationProlactin receptor signalingINS-1 cellsGestational diabetesInsulin resistanceReceptor upregulationGlucose homeostasisBiphasic responseThe spectrum of cutaneous immune-related adverse events in patients with skin of color
Belzer A, Mortlock R, Olamiju B, Olino K, Cohen J, Leventhal J. The spectrum of cutaneous immune-related adverse events in patients with skin of color. Journal Of The American Academy Of Dermatology 2022, 88: 1156-1158. PMID: 36470309, PMCID: PMC10838520, DOI: 10.1016/j.jaad.2022.11.051.Peer-Reviewed Original ResearchThe effect of baseline eczema or psoriasis on the morphology of cutaneous immune-related adverse events due to immune checkpoint inhibitor therapy
Belzer A, Mortlock R, Pach J, Cohen J, Leventhal J. The effect of baseline eczema or psoriasis on the morphology of cutaneous immune-related adverse events due to immune checkpoint inhibitor therapy. Journal Of The American Academy Of Dermatology 2023, 88: 1198-1200. PMID: 36634749, PMCID: PMC10121758, DOI: 10.1016/j.jaad.2023.01.002.Peer-Reviewed Original ResearchAssessment of Treatment-Relevant Immune Biomarkers in Psoriasis and Atopic Dermatitis: Toward Personalized Medicine in Dermatology
Mortlock R, Ma E, Cohen J, Damsky W. Assessment of Treatment-Relevant Immune Biomarkers in Psoriasis and Atopic Dermatitis: Toward Personalized Medicine in Dermatology. Journal Of Investigative Dermatology 2023, 143: 1412-1422. PMID: 37341663, PMCID: PMC10830170, DOI: 10.1016/j.jid.2023.04.005.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsAtopic dermatitisSkin diseasesInflammatory skin diseaseInflammatory skin conditionImmunologic biomarkersImmune biomarkersPersonalized medicineInflammatory dermatosesImmunologic approachesTherapy selectionSkin conditionsSingle-cell RNA sequencingMolecular profilingBiomarkersTissue stainingPsoriasisDermatitisDiseaseDermatologyRNA sequencingMedicineCurettageDermatosesTherapyPersonalized classificationInterrogation of clonal tracking data using barcodetrackR
Espinoza D, Mortlock R, Koelle S, Wu C, Dunbar C. Interrogation of clonal tracking data using barcodetrackR. Nature Computational Science 2021, 1: 280-289. PMID: 37621673, PMCID: PMC10449013, DOI: 10.1038/s43588-021-00057-4.Peer-Reviewed Original ResearchProgenitor cellsClonal tracking studiesProgenitor cell outputContext of genesHSPC transplantCellular compartmentsCellular outputStandardized analytical frameworkLineage relationshipsHematopoietic stemClonal dynamicsHSPC gene therapyLineage reconstitutionR packageVector genotoxicityCellsShiny appTumor cellsClonal patternGene therapyTracking dataDiverse toolsGenesQuantitative insightsValuable insights
2024
Enhancing in vivo cell and tissue targeting by modulation of polymer nanoparticles and macrophage decoys
Piotrowski-Daspit A, Bracaglia L, Eaton D, Richfield O, Binns T, Albert C, Gould J, Mortlock R, Egan M, Pober J, Saltzman W. Enhancing in vivo cell and tissue targeting by modulation of polymer nanoparticles and macrophage decoys. Nature Communications 2024, 15: 4247. PMID: 38762483, PMCID: PMC11102454, DOI: 10.1038/s41467-024-48442-7.Peer-Reviewed Original ResearchConceptsPoly(amine-co-esterPolymer nanoparticlesDelivery of nucleic acid therapeuticsCell-type tropismTissue tropismNucleic acid delivery vehiclesIn vivo deliveryIn vivo efficacyCirculation half-lifeNucleic acid therapeuticsVehicle characteristicsTunable propertiesBiodistribution assessmentPhysiological fatePolymer chemistrySurface propertiesPharmacokinetic modelTissue targetingNanoparticlesDistribution modifiersPolymeric nanoparticlesTropismPolymerDelivery vehiclesHalf-lifeImpact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Lee B, Gin A, Wu C, Singh K, Grice M, Mortlock R, Abraham D, Fan X, Zhou Y, AlJanahi A, Choi U, DeRavin S, Shin T, Hong S, Dunbar C. Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques. Cell Stem Cell 2024, 31: 788. PMID: 38599215, PMCID: PMC11114628, DOI: 10.1016/j.stem.2024.04.005.Peer-Reviewed Original ResearchImpact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Lee B, Gin A, Wu C, Singh K, Grice M, Mortlock R, Abraham D, Fan X, Zhou Y, AlJanahi A, Choi U, DeRavin S, Shin T, Hong S, Dunbar C. Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques. Cell Stem Cell 2024, 31: 455-466.e4. PMID: 38508195, PMCID: PMC10997443, DOI: 10.1016/j.stem.2024.02.010.Peer-Reviewed Original ResearchLentiviral vectorsRhesus macaquesGFP-expressing lentiviral vectorsClonal dynamicsLong-term engrafting hematopoietic stem cellsLong-term engraftmentHematopoietic stem cellsAutologous transplantation modelClinically relevant differencesLT-HSCLT-HSCsCells long-termTransplantation modelLentiviral transductionP53 inhibitionAnimal modelsStem cellsGenetic modification approachesRelevant differencesClonalityHSCCellsMacaquesEngraftmentCD33How Efforts to Understand Somatic Mosaicism Will Impact Dermatology
Mortlock R, Choate K. How Efforts to Understand Somatic Mosaicism Will Impact Dermatology. Journal Of Investigative Dermatology 2024, 144: 453-455. PMID: 38395493, PMCID: PMC11009867, DOI: 10.1016/j.jid.2023.10.007.Peer-Reviewed Original Research
2023
Early observations with an ERAS pathway for thyroid and parathyroid surgery: Moving the goalposts forward
Machado N, Mortlock R, Maduka R, Souza Cunha A, Dyer E, Long A, Canner J, Tanella A, Gibson C, Hyman J, Ogilvie J. Early observations with an ERAS pathway for thyroid and parathyroid surgery: Moving the goalposts forward. Surgery 2023, 175: 114-120. PMID: 37973430, PMCID: PMC10838521, DOI: 10.1016/j.surg.2023.06.052.Peer-Reviewed Original ResearchConceptsSurgery pathwayEnhanced recoverySurgery groupParathyroid surgerySignificant decreasePost-anesthesia care unit lengthAverage daily pain scoreSuperficial cervical plexus blockThyroid/parathyroid surgeryAverage daily morphineDaily pain scoresCervical plexus blockCases 1 yearStandard of careDaily morphineERAS pathwayPostoperative admissionPostoperative antiemeticsPostoperative morphineUnanticipated admissionPain scoresPerioperative outcomesPlexus blockProspective trialPatient groupEvaluating the medical management of locally advanced and metastatic basal cell carcinoma: A single institutional retrospective analysis investigating efficacy, safety, and tolerability
Belzer A, Pach J, Mortlock R, Clune J, Olino K, Sznol M, Bhatia A, Burtness B, Christensen S, Leventhal J. Evaluating the medical management of locally advanced and metastatic basal cell carcinoma: A single institutional retrospective analysis investigating efficacy, safety, and tolerability. JAAD International 2023, 11: 174-175. PMID: 37252181, PMCID: PMC10213716, DOI: 10.1016/j.jdin.2023.02.007.Peer-Reviewed Original Research
2022
Expanded NK cells used for adoptive cell therapy maintain diverse clonality and contain long-lived memory-like NK cell populations
Allan D, Wu C, Mortlock R, Chakraborty M, Rezvani K, Davidson-Moncada J, Dunbar C, Childs R. Expanded NK cells used for adoptive cell therapy maintain diverse clonality and contain long-lived memory-like NK cell populations. Molecular Therapy — Oncolytics 2022, 28: 74-87. PMID: 36699615, PMCID: PMC9842935, DOI: 10.1016/j.omto.2022.12.006.Peer-Reviewed Original ResearchComparison of busulfan and total body irradiation conditioning on hematopoietic clonal dynamics following lentiviral gene transfer in rhesus macaques
Abraham D, Lozano R, Guitart X, Liang J, Mortlock R, Espinoza D, Fan X, Krouse A, Bonifacino A, Hong S, Singh K, Tisdale J, Wu C, Dunbar C. Comparison of busulfan and total body irradiation conditioning on hematopoietic clonal dynamics following lentiviral gene transfer in rhesus macaques. Molecular Therapy — Methods & Clinical Development 2022, 28: 62-75. PMID: 36620072, PMCID: PMC9798201, DOI: 10.1016/j.omtm.2022.12.001.Peer-Reviewed Original ResearchBarcode clonal tracking of tissue-resident immune cells in rhesus macaque highlights distinct clonal distribution pattern of tissue NK cells
Wu C, Liang J, Brenchley J, Shin T, Fan X, Mortlock R, Abraham D, Allan D, Thomas M, Hong S, Dunbar C. Barcode clonal tracking of tissue-resident immune cells in rhesus macaque highlights distinct clonal distribution pattern of tissue NK cells. Frontiers In Immunology 2022, 13: 994498. PMID: 36605190, PMCID: PMC9808525, DOI: 10.3389/fimmu.2022.994498.Peer-Reviewed Original ResearchConceptsTissue-resident immune cellsNK cellsPeripheral bloodCD56-CD16NK memoryNK clonesImmune cellsImmunological memoryT cellsNatural killer cell populationsClonal expansionDevelopment of NKTissue NK cellsResident immune cellsClonal distributionKiller cell populationsBlood T cellsCD16-CD56NK subsetsAutologous transplantationImmune responseGastrointestinal tractGI tractMyeloid cellsDN populationTissue Trafficking Kinetics of Rhesus Macaque Natural Killer Cells Measured by Serial Intravascular Staining
Mortlock RD, Wu C, Potter EL, Abraham DM, Allan DSJ, Hong SG, Roederer M, Dunbar CE. Tissue Trafficking Kinetics of Rhesus Macaque Natural Killer Cells Measured by Serial Intravascular Staining. Frontiers In Immunology 2022, 12: 772332. PMID: 35095846, PMCID: PMC8790741, DOI: 10.3389/fimmu.2021.772332.Peer-Reviewed Original ResearchConceptsNatural killer cellsNK cellsKiller cellsPeripheral bloodIntravascular stainingHuman NK cell biologyRhesus macaquesNK cell subsetsNK cell biologyTissue distributionNon-lymphoid tissuesNK compartmentLymph nodesCell subsetsAnimal modelsRM tissuesCD16Perivascular compartmentTrafficking patternsTissueBloodHigher proportionStainingCellsDifferent subsets