Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Lee B, Gin A, Wu C, Singh K, Grice M, Mortlock R, Abraham D, Fan X, Zhou Y, AlJanahi A, Choi U, DeRavin S, Shin T, Hong S, Dunbar C. Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques. Cell Stem Cell 2024, 31: 455-466.e4. PMID: 38508195, PMCID: PMC10997443, DOI: 10.1016/j.stem.2024.02.010.Peer-Reviewed Original ResearchLentiviral vectorsRhesus macaquesGFP-expressing lentiviral vectorsClonal dynamicsLong-term engrafting hematopoietic stem cellsLong-term engraftmentHematopoietic stem cellsAutologous transplantation modelClinically relevant differencesLT-HSCLT-HSCsCells long-termTransplantation modelLentiviral transductionP53 inhibitionAnimal modelsStem cellsGenetic modification approachesRelevant differencesClonalityHSCCellsMacaquesEngraftmentCD33