2024
Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques
Lee B, Gin A, Wu C, Singh K, Grice M, Mortlock R, Abraham D, Fan X, Zhou Y, AlJanahi A, Choi U, DeRavin S, Shin T, Hong S, Dunbar C. Impact of CRISPR/HDR editing versus lentiviral transduction on long-term engraftment and clonal dynamics of HSPCs in rhesus macaques. Cell Stem Cell 2024, 31: 455-466.e4. PMID: 38508195, PMCID: PMC10997443, DOI: 10.1016/j.stem.2024.02.010.Peer-Reviewed Original ResearchLentiviral vectorsRhesus macaquesGFP-expressing lentiviral vectorsClonal dynamicsLong-term engrafting hematopoietic stem cellsLong-term engraftmentHematopoietic stem cellsAutologous transplantation modelClinically relevant differencesLT-HSCLT-HSCsCells long-termTransplantation modelLentiviral transductionP53 inhibitionAnimal modelsStem cellsGenetic modification approachesRelevant differencesClonalityHSCCellsMacaquesEngraftmentCD33
2022
Tissue Trafficking Kinetics of Rhesus Macaque Natural Killer Cells Measured by Serial Intravascular Staining
Mortlock RD, Wu C, Potter EL, Abraham DM, Allan DSJ, Hong SG, Roederer M, Dunbar CE. Tissue Trafficking Kinetics of Rhesus Macaque Natural Killer Cells Measured by Serial Intravascular Staining. Frontiers In Immunology 2022, 12: 772332. PMID: 35095846, PMCID: PMC8790741, DOI: 10.3389/fimmu.2021.772332.Peer-Reviewed Original ResearchConceptsNatural killer cellsNK cellsKiller cellsPeripheral bloodIntravascular stainingHuman NK cell biologyRhesus macaquesNK cell subsetsNK cell biologyTissue distributionNon-lymphoid tissuesNK compartmentLymph nodesCell subsetsAnimal modelsRM tissuesCD16Perivascular compartmentTrafficking patternsTissueBloodHigher proportionStainingCellsDifferent subsets