2020
DNA aptamer-based non-faradaic impedance biosensor for detecting E. coli
Abdelrasoul GN, Anwar A, MacKay S, Tamura M, Shah MA, Khasa DP, Montgomery RR, Ko AI, Chen J. DNA aptamer-based non-faradaic impedance biosensor for detecting E. coli. Analytica Chimica Acta 2020, 1107: 135-144. PMID: 32200887, DOI: 10.1016/j.aca.2020.02.004.Peer-Reviewed Original ResearchConceptsField-emission scanning electron microscopyImpedance-based biosensorAtomic force microscopySelf-assembled monolayersInterdigitated ElectrodesIDE surfaceSubstrate materialEmission scanning electron microscopyMolecular recognition elementsEnzyme-linked immunosorbent assayConventional detection approachesScanning electron microscopyIDE arrayImpedance biosensorLimit of detectionForce microscopyBiosensorSurface chemistryAptamerPolymerase chain reaction assaysRecognition elementThiol mixturesElectron microscopyEnvironmental monitoringChain reaction assays
2004
TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi
Pal U, Li X, Wang T, Montgomery RR, Ramamoorthi N, deSilva AM, Bao F, Yang X, Pypaert M, Pradhan D, Kantor FS, Telford S, Anderson JF, Fikrig E. TROSPA, an Ixodes scapularis Receptor for Borrelia burgdorferi. Cell 2004, 119: 457-468. PMID: 15537536, DOI: 10.1016/j.cell.2004.10.027.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBase SequenceBorrelia burgdorferiCloning, MolecularGene Expression RegulationHost-Parasite InteractionsIntestinesIxodesLipoproteinsMiceMice, Inbred C3HMolecular Sequence DataReceptors, Cell SurfaceRecombinant ProteinsConceptsLyme disease agent Borrelia burgdorferiSurvival of spirochetesTick receptorMammalian hostsRNA interferenceEfficient colonizationTROSPAOuter surface protein ABorrelia burgdorferiPathogen adherencePathogen transmissionProtein AB. burgdorferi outer surface protein AMRNA levelsIxodes scapularisB. burgdorferiColonizationSurface protein AReceptorsArthropodsBurgdorferiMammalsRepressionSpirochetesTicks
2000
Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A
Pal U, de Silva A, Montgomery R, Fish D, Anguita J, Anderson J, Lobet Y, Fikrig E. Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A. Journal Of Clinical Investigation 2000, 106: 561-569. PMID: 10953031, PMCID: PMC380253, DOI: 10.1172/jci9427.Peer-Reviewed Original Research
1996
Direct demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease.
Montgomery RR, Malawista SE, Feen KJ, Bockenstedt LK. Direct demonstration of antigenic substitution of Borrelia burgdorferi ex vivo: exploration of the paradox of the early immune response to outer surface proteins A and C in Lyme disease. Journal Of Experimental Medicine 1996, 183: 261-269. PMID: 8551229, PMCID: PMC2192432, DOI: 10.1084/jem.183.1.261.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, BiologicalAnimalsAntibodies, BacterialAntibody FormationAntigens, BacterialAntigens, SurfaceBacterial Outer Membrane ProteinsBacterial VaccinesBase SequenceFemaleFluorescent Antibody TechniqueGene ExpressionLipoproteinsLyme DiseaseMiceMice, Inbred C3HMolecular Sequence DataPeritoneal CavityPolymerase Chain ReactionRNA, MessengerSpecific Pathogen-Free OrganismsConceptsOsp AA antibodiesImmune responseOuter surface proteinsLyme diseaseWk of infectionProtective immune responseEarly immune responseReverse transcription-polymerase chain reactionStrong humoral responseB. burgdorferi strain N40Transcription-polymerase chain reactionDirect fluorescent stainingHumoral responsePolymerase chain reactionSurface proteinsEarly courseDay 14Etiologic agentDay 30Vaccine designEx vivoIndirect immunofluorescenceInfectionImmune repertoire
1994
Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease
Lam TT, Nguyen TP, Montgomery RR, Kantor FS, Fikrig E, Flavell RA. Outer surface proteins E and F of Borrelia burgdorferi, the agent of Lyme disease. Infection And Immunity 1994, 62: 290-298. PMID: 8262642, PMCID: PMC186099, DOI: 10.1128/iai.62.1.290-298.1994.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, BacterialBacterial Outer Membrane ProteinsBacterial ProteinsBase SequenceBlotting, WesternBorrelia burgdorferi GroupCloning, MolecularCodonFluorescent Antibody TechniqueGenes, BacterialHumansLipoproteinsLyme DiseaseMolecular Sequence DataMolecular WeightOperonRegulatory Sequences, Nucleic AcidRestriction MappingSequence AlignmentSequence Homology, Nucleic AcidSolubilityConceptsOspE genesMolecular massSignal peptidase IIConsensus cleavage sequenceTranscriptional unitsLeader sequenceCommon promoterBp downstreamOuter surface proteinsProtein EStop codonSurface lipoproteinsLabeling showBorrelia burgdorferiGenesHydrophobic domainCleavage sequenceSurface proteinsAmino acidsPeptidase IIProteinOuter surface protein EGel electrophoresisNucleotidesImmunofluorescence studies