2014
Reduced bioenergetics and toll-like receptor 1 function in human polymorphonuclear leukocytes in aging
Qian F, Guo X, Wang X, Yuan X, Chen S, Malawista SE, Bockenstedt LK, Allore HG, Montgomery RR. Reduced bioenergetics and toll-like receptor 1 function in human polymorphonuclear leukocytes in aging. Aging 2014, 6: 131-139. PMID: 24595889, PMCID: PMC3969281, DOI: 10.18632/aging.100642.Peer-Reviewed Original ResearchConceptsToll-like receptor 1Polymorphonuclear leukocytesOlder adultsChemokine IL-8Loss of CD62LAge-dependent deficienciesPotential therapeutic opportunitiesHuman polymorphonuclear leukocytesTLR1 expressionIL-8PMN functionPMN activationMultiple pathwaysImmune functionYoung donorsP38 MAP kinaseReceptor 1Bacterial infectionsTherapeutic opportunitiesProgressive declineGlucose uptakeReduced expressionAdultsStimulationLeukocytes
2009
Inhibition of Neutrophil Function by Two Tick Salivary Proteins
Guo X, Booth CJ, Paley MA, Wang X, DePonte K, Fikrig E, Narasimhan S, Montgomery RR. Inhibition of Neutrophil Function by Two Tick Salivary Proteins. Infection And Immunity 2009, 77: 2320-2329. PMID: 19332533, PMCID: PMC2687334, DOI: 10.1128/iai.01507-08.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesPMN functionNumber of PMNPMN integrinsPMN adherenceNeutrophil functionSpirochete burdenTick salivary proteinsTick salivaLyme diseaseTick attachmentSalivary glandsBorrelia burgdorferiTick feedingCausative agentReduced levelsInhibitory proteinSalivaBlood mealAntihemostatic activityInfectionInhibitionSalivary proteinsHematophagous arthropodsTick Ixodes scapularis
2006
Recruitment of Macrophages and Polymorphonuclear Leukocytes in Lyme Carditis
Montgomery RR, Booth CJ, Wang X, Blaho VA, Malawista SE, Brown CR. Recruitment of Macrophages and Polymorphonuclear Leukocytes in Lyme Carditis. Infection And Immunity 2006, 75: 613-620. PMID: 17101663, PMCID: PMC1828503, DOI: 10.1128/iai.00685-06.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBorrelia burgdorferiColony Count, MicrobialCytokinesDisease Models, AnimalDisease SusceptibilityDNA, BacterialHeartHistocytochemistryLyme DiseaseMacrophagesMiceMice, Inbred C3HMice, Inbred C57BLMice, KnockoutMyocarditisMyocardiumNeutrophilsPolymerase Chain ReactionReceptors, CCR2Receptors, ChemokineUrinary BladderConceptsLyme carditisPolymorphonuclear leukocytesC3H micePresence of PMNsB. burgdorferi burdenNeutrophil chemokine receptorOrgan-specific pathogenesisChemokine receptor CCR2B. burgdorferiRecruitment of macrophagesWild-type miceB. burgdorferi infectionAbsence of macrophagesFunction of macrophagesPeak diseaseInfected heartsLyme arthritisSevere arthritisHeart lesionsReceptor CCR2Severe inflammationHistopathologic examinationChemokine receptorsBurgdorferi infectionCarditis
2003
Calprotectin, an Abundant Cytosolic Protein from Human Polymorphonuclear Leukocytes, Inhibits the Growth of Borrelia burgdorferi
Lusitani D, Malawista SE, Montgomery RR. Calprotectin, an Abundant Cytosolic Protein from Human Polymorphonuclear Leukocytes, Inhibits the Growth of Borrelia burgdorferi. Infection And Immunity 2003, 71: 4711-4716. PMID: 12874352, PMCID: PMC166021, DOI: 10.1128/iai.71.8.4711-4716.2003.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesB. burgdorferiGranule-poor cytoplastsBorrelia burgdorferiBlood polymorphonuclear leukocytesHuman blood polymorphonuclear leukocytesNumerous polymorphonuclear leukocytesPotent bacteriostatic agentHuman polymorphonuclear leukocytesU-CytAcute inflammationCalprotectinModulatory roleLyme spirocheteLyme diseaseSpecific antibodiesProtein calprotectinBurgdorferiLeukocytesAbundant cytosolic proteinOxidative mechanismsBacteriostatic agentRegrowth assaysQuantitative microscopicInflammation
2002
Human phagocytic cells in the early innate immune response to Borrelia burgdorferi
Montgomery RR, Lusitani D, de Boisfleury Chevance A, Malawista SE. Human phagocytic cells in the early innate immune response to Borrelia burgdorferi. The Journal Of Infectious Diseases 2002, 185: 1773-1779. PMID: 12085324, DOI: 10.1086/340826.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesImmune responseEarly innate immune responseKilling of spirochetesSpecific antibodiesBorrelia burgdorferiSecondary immune responseInnate immune responseInnate immune systemHuman phagocytic cellsSpirochete clearanceMononuclear cellsImmune systemLyme diseasePhagocytic cellsNatural infectionMature macrophagesSpirochetesIntracellular colocalizationAntibodiesBurgdorferiFirst cellsLimited uptakeCellsMonocytesBorrelia burgdorferi Are Susceptible to Killing by a Variety of Human Polymorphonuclear Leukocyte Components
Lusitani D, Malawista SE, Montgomery RR. Borrelia burgdorferi Are Susceptible to Killing by a Variety of Human Polymorphonuclear Leukocyte Components. The Journal Of Infectious Diseases 2002, 185: 797-804. PMID: 11920297, DOI: 10.1086/339341.Peer-Reviewed Original ResearchConceptsIntact polymorphonuclear leukocytesPolymorphonuclear leukocytesB. burgdorferiKilling of BorreliaHuman neutrophil peptides 1Neutrophil peptide-1Bactericidal/permeability-increasing proteinIntact human polymorphonuclear leukocytesHuman polymorphonuclear leukocytesPermeability-increasing proteinProteinase 3Peptide-1Leukocyte componentsNitric oxideLL-37Specific antibodiesBorrelia burgdorferiBurgdorferiGranule contentsEfficient killingSpirochetesCritical roleKillingLeukocytes
1992
Evidence for reactive nitrogen intermediates in killing of staphylococci by human neutrophil cytoplasts. A new microbicidal pathway for polymorphonuclear leukocytes.
Malawista SE, Montgomery RR, van Blaricom G. Evidence for reactive nitrogen intermediates in killing of staphylococci by human neutrophil cytoplasts. A new microbicidal pathway for polymorphonuclear leukocytes. Journal Of Clinical Investigation 1992, 90: 631-636. PMID: 1379614, PMCID: PMC443143, DOI: 10.1172/jci115903.Peer-Reviewed Original ResearchConceptsReactive nitrogen intermediatesL-arginineNitric oxide synthase inhibitor NGranule-poor cytoplastsKilling of staphylococciNitrogen intermediatesSynthase inhibitor NEffect of NMMAChronic granulomatous diseaseHuman blood neutrophilsHuman neutrophil cytoplastsBlood neutrophilsGranulomatous diseasePolymorphonuclear leukocytesD-arginineIntact PMNsNeutrophil cytoplastsMicrobicidal pathwaysNMMASusceptible targetsMin of incubationModest decreaseInhibitor NStaphylococciOxidase activity