2023
In vivo imaging of cerebral glucose metabolism informs on subacute to chronic post-stroke tissue status – A pilot study combining PET and deuterium metabolic imaging
Meerwaldt A, Straathof M, Oosterveld W, van Heijningen C, van Leent M, Toner Y, Munitz J, Teunissen A, Daemen C, van der Toorn A, van Vliet G, van Tilborg G, De Feyter H, de Graaf R, Hol E, Mulder W, Dijkhuizen R. In vivo imaging of cerebral glucose metabolism informs on subacute to chronic post-stroke tissue status – A pilot study combining PET and deuterium metabolic imaging. Cerebrovascular And Brain Metabolism Reviews 2023, 43: 778-790. PMID: 36606595, PMCID: PMC10108187, DOI: 10.1177/0271678x221148970.Peer-Reviewed Original ResearchConceptsTransient middle cerebral artery occlusionPositron emission tomographyGlucose metabolismPost-ischemic brain tissueMiddle cerebral artery occlusionFDG positron emission tomographyFluorodeoxyglucose positron emission tomographyMetabolic imagingSignificant glial activationAcute ischemic strokeCerebral artery occlusionCerebral energy metabolismDeuterium metabolic imagingActive glucose metabolismGlial activationRecanalization therapyArtery occlusionIschemic strokeStroke severityCerebral perfusionC57BL/6 micePoor outcomeElevated lactate productionPathophysiological changesBaseline values
2021
NMR visibility of deuterium‐labeled liver glycogen in vivo
De Feyter HM, Thomas MA, Behar KL, de Graaf R. NMR visibility of deuterium‐labeled liver glycogen in vivo. Magnetic Resonance In Medicine 2021, 86: 62-68. PMID: 33590529, PMCID: PMC8005460, DOI: 10.1002/mrm.28717.Peer-Reviewed Original Research
2020
B0 shimming for in vivo magnetic resonance spectroscopy: Experts' consensus recommendations
Juchem C, Cudalbu C, de Graaf R, Gruetter R, Henning A, Hetherington H, Boer V. B0 shimming for in vivo magnetic resonance spectroscopy: Experts' consensus recommendations. NMR In Biomedicine 2020, 34: e4350. PMID: 32596978, DOI: 10.1002/nbm.4350.Peer-Reviewed Original ResearchIn vivo MRS measurement of 2‐hydroxyglutarate in patient‐derived IDH‐mutant xenograft mouse models versus glioma patients
Tiwari V, Mashimo T, An Z, Vemireddy V, Piccirillo S, Askari P, Hulsey K, Zhang S, de Graaf R, Patel T, Pan E, Mickey B, Maher E, Bachoo R, Choi C. In vivo MRS measurement of 2‐hydroxyglutarate in patient‐derived IDH‐mutant xenograft mouse models versus glioma patients. Magnetic Resonance In Medicine 2020, 84: 1152-1160. PMID: 32003035, PMCID: PMC7263951, DOI: 10.1002/mrm.28183.Peer-Reviewed Original ResearchConceptsPatient-derived xenograftsPatient-derived xenograft micePreclinical modelsGlioma patientsPatient-derived xenograft tumorsPatient-derived xenograft modelsDetection of 2HGIDH1 R132H mutationShort-TE MRSXenograft mouse modelMutant gliomasR132H mutationIDH-mutantIn vivo MRS measurementsMouse modelPhantom analysisMetabolic reprogrammingPatientsMetabolite levelsGliomaMiceMetabolic fingerprintsPRESS sequenceIsocitrate dehydrogenaseMRS methods
2019
On the magnetic field dependence of deuterium metabolic imaging
de Graaf R, Hendriks AD, Klomp DWJ, Kumaragamage C, Welting D, de Castro C, Brown PB, McIntyre S, Nixon TW, Prompers JJ, De Feyter HM. On the magnetic field dependence of deuterium metabolic imaging. NMR In Biomedicine 2019, 33: e4235. PMID: 31879985, PMCID: PMC7141779, DOI: 10.1002/nbm.4235.Peer-Reviewed Original ResearchIn vivo 13C and 1H‐[13C] MRS studies of neuroenergetics and neurotransmitter cycling, applications to neurological and psychiatric disease and brain cancer
Rothman DL, de Graaf R, Hyder F, Mason GF, Behar KL, De Feyter HM. In vivo 13C and 1H‐[13C] MRS studies of neuroenergetics and neurotransmitter cycling, applications to neurological and psychiatric disease and brain cancer. NMR In Biomedicine 2019, 32: e4172. PMID: 31478594, DOI: 10.1002/nbm.4172.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2018
Deuterium metabolic imaging (DMI) for MRI-based 3D mapping of metabolism in vivo
De Feyter HM, Behar KL, Corbin ZA, Fulbright RK, Brown PB, McIntyre S, Nixon TW, Rothman DL, de Graaf RA. Deuterium metabolic imaging (DMI) for MRI-based 3D mapping of metabolism in vivo. Science Advances 2018, 4: eaat7314. PMID: 30140744, PMCID: PMC6105304, DOI: 10.1126/sciadv.aat7314.Peer-Reviewed Original ResearchConceptsOral intakeMetabolic imagingGlucose uptakeHigh-grade brain tumorsRat glioma modelPositron emission tomography (PET) detectionSimilar metabolic patternMetabolic imaging techniquesDeuterium metabolic imagingHigher glucose uptakeGlucose analog 2FDG-PETF-fluoroIntravenous infusionBrain tumorsGlioma modelGlucose metabolismNormal brainTomography detectionAnimal modelsMagnetic resonance spectroscopicTumor tissueHuman liverMetabolic patternsNoninvasive approachMinimum echo time PRESS‐based proton observed carbon edited (POCE) MRS in rat brain using simultaneous editing and localization pulses
Kumaragamage C, Madularu D, Mathieu AP, Lupinsky D, de Graaf R, Near J. Minimum echo time PRESS‐based proton observed carbon edited (POCE) MRS in rat brain using simultaneous editing and localization pulses. Magnetic Resonance In Medicine 2018, 80: 1279-1288. PMID: 29427395, DOI: 10.1002/mrm.27119.Peer-Reviewed Original Research
2014
Detection of cerebral NAD+ by in vivo 1H NMR spectroscopy
de Graaf R, Behar KL. Detection of cerebral NAD+ by in vivo 1H NMR spectroscopy. NMR In Biomedicine 2014, 27: 802-809. PMID: 24831866, PMCID: PMC4459131, DOI: 10.1002/nbm.3121.Peer-Reviewed Original Research
2013
In vivo three‐dimensional molecular imaging with Biosensor Imaging of Redundant Deviation in Shifts (BIRDS) at high spatiotemporal resolution
Coman D, de Graaf R, Rothman DL, Hyder F. In vivo three‐dimensional molecular imaging with Biosensor Imaging of Redundant Deviation in Shifts (BIRDS) at high spatiotemporal resolution. NMR In Biomedicine 2013, 26: 1589-1595. PMID: 23881869, PMCID: PMC3800475, DOI: 10.1002/nbm.2995.Peer-Reviewed Original ResearchConceptsK-spaceNonexchanging protonsThree-dimensional molecular imagingParamagnetic lanthanide ionsRedundant deviationChemical shift imagingField of viewMolecular imaging capabilitiesMolecular imagingRapid data acquisitionProton detectionLanthanide ionsTens of minutesImaging capabilitiesMacrocyclic chelatesSpectroscopic signalsMacrocyclicCSI experimentsRelaxation timeHigh spatiotemporal resolutionParamagnetic agents
2012
In vivo MRS and histochemistry of status epilepticus‐induced hippocampal pathology in a juvenile model of temporal lobe epilepsy
van der Hel W, van Eijsden P, Bos IW, de Graaf R, Behar KL, van Nieuwenhuizen O, de Graan P, Braun KP. In vivo MRS and histochemistry of status epilepticus‐induced hippocampal pathology in a juvenile model of temporal lobe epilepsy. NMR In Biomedicine 2012, 26: 132-140. PMID: 22806932, DOI: 10.1002/nbm.2828.Peer-Reviewed Original ResearchConceptsTemporal lobe epilepsyStatus epilepticusSynthase immunoreactivityNeuronal lossHippocampal pathologyLobe epilepsyDevelopment of TLECholine-containing compound concentrationsChildhood status epilepticusPre-epileptic stateSpontaneous recurrent seizuresVivo MRSJuvenile rat modelHippocampal injuryChronic epilepsyRecurrent seizuresSpontaneous seizuresNeurotransmitter imbalanceParvalbumin immunoreactivityVivo immunohistochemistryInhibitory neurotransmissionΓ-aminobutyric acidEpileptogenic processMRS findingsNeuronal death
2011
13C MRS studies of neuroenergetics and neurotransmitter cycling in humans
Rothman DL, De Feyter HM, de Graaf R, Mason GF, Behar KL. 13C MRS studies of neuroenergetics and neurotransmitter cycling in humans. NMR In Biomedicine 2011, 24: 943-957. PMID: 21882281, PMCID: PMC3651027, DOI: 10.1002/nbm.1772.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsNeurotransmitter cyclingMRS studiesHigh neuronal activityHuman brain diseasesOnly noninvasive methodBrain metabolismGlutamate neurotransmissionNeuronal activityPsychiatric diseasesNeuronal functionBrain diseasesBrain functionClinical researchNeuroenergeticsNoninvasive methodMRSMRS measurementsHuman researchDiseaseBrain statesRecent findingsImportant new informationHumansMetabolic pathwaysNeurotransmissionDynamic multi-coil shimming of the human brain at 7T
Juchem C, Nixon TW, McIntyre S, Boer VO, Rothman DL, de Graaf RA. Dynamic multi-coil shimming of the human brain at 7T. Journal Of Magnetic Resonance 2011, 212: 280-288. PMID: 21824794, PMCID: PMC3183127, DOI: 10.1016/j.jmr.2011.07.005.Peer-Reviewed Original Research
2009
In vivo neurochemical profiling of rat brain by 1H‐[13C] NMR spectroscopy: cerebral energetics and glutamatergic/GABAergic neurotransmission
Van Eijsden P, Behar KL, Mason GF, Braun KP, De Graaf RA. In vivo neurochemical profiling of rat brain by 1H‐[13C] NMR spectroscopy: cerebral energetics and glutamatergic/GABAergic neurotransmission. Journal Of Neurochemistry 2009, 112: 24-33. PMID: 19818103, PMCID: PMC2843425, DOI: 10.1111/j.1471-4159.2009.06428.x.Peer-Reviewed Original ResearchConceptsGABAergic neurotransmissionRat brainGlutamatergic/GABAergic neurotransmissionLow GABA concentrationsInhibitory neurotransmissionGlutamatergic neurotransmissionCerebral energeticsGABA concentrationNeurotransmissionBrain functionNeurotransmitter fluxEnergy metabolismNeurochemical profilingVivoBrainHigh magnetic fieldsGABAergicExcitatoryMagnetic fieldIn situ 3D magnetic resonance metabolic imaging of microwave‐irradiated rodent brain: a new tool for metabolomics research
De Graaf RA, Chowdhury GM, Brown PB, Rothman DL, Behar KL. In situ 3D magnetic resonance metabolic imaging of microwave‐irradiated rodent brain: a new tool for metabolomics research. Journal Of Neurochemistry 2009, 109: 494-501. PMID: 19200336, PMCID: PMC2843429, DOI: 10.1111/j.1471-4159.2009.05967.x.Peer-Reviewed Original Research
2003
Coupling of Glutamatergic Neurotransmission and Neuronal Glucose Oxidation over the Entire Range of Cerebral Cortex Activity
PATEL AB, DE GRAAF R, MASON GF, ROTHMAN DL, SHULMAN RG, BEHAR KL. Coupling of Glutamatergic Neurotransmission and Neuronal Glucose Oxidation over the Entire Range of Cerebral Cortex Activity. Annals Of The New York Academy Of Sciences 2003, 1003: 452-453. PMID: 14684486, DOI: 10.1196/annals.1300.050.Peer-Reviewed Original Research
2001
Diffusion NMR spectroscopy
Nicolay K, Braun K, de Graaf R, Dijkhuizen R, Kruiskamp M. Diffusion NMR spectroscopy. NMR In Biomedicine 2001, 14: 94-111. PMID: 11320536, DOI: 10.1002/nbm.686.Peer-Reviewed Original ResearchSingle‐shot diffusion trace 1H NMR spectroscopy
de Graaf R, Braun K, Nicolay K. Single‐shot diffusion trace 1H NMR spectroscopy. Magnetic Resonance In Medicine 2001, 45: 741-748. PMID: 11323799, DOI: 10.1002/mrm.1101.Peer-Reviewed Original Research
2000
In Vivo 31P-NMR Diffusion Spectroscopy of ATP and Phosphocreatine in Rat Skeletal Muscle
de Graaf R, van Kranenburg A, Nicolay K. In Vivo 31P-NMR Diffusion Spectroscopy of ATP and Phosphocreatine in Rat Skeletal Muscle. Biophysical Journal 2000, 78: 1657-1664. PMID: 10733948, PMCID: PMC1300762, DOI: 10.1016/s0006-3495(00)76717-8.Peer-Reviewed Original Research
1999
Magnetic coupling of creatine/phosphocreatine protons in rat skeletal muscle, as studied by 1H‐magnetization transfer MRS
Kruiskamp M, de Graaf R, van Vliet G, Nicolay K. Magnetic coupling of creatine/phosphocreatine protons in rat skeletal muscle, as studied by 1H‐magnetization transfer MRS. Magnetic Resonance In Medicine 1999, 42: 665-672. PMID: 10502754, DOI: 10.1002/(sici)1522-2594(199910)42:4<665::aid-mrm7>3.0.co;2-9.Peer-Reviewed Original Research