2009
Skin immune surveillance by T cells—A new order?
Strid J, Tigelaar RE, Hayday AC. Skin immune surveillance by T cells—A new order? Seminars In Immunology 2009, 21: 110-120. PMID: 19369094, DOI: 10.1016/j.smim.2009.03.002.Peer-Reviewed Original ResearchConceptsImmune surveillanceAdaptive immune surveillanceStress-induced antigensSystemic immune cellsSkin immune surveillanceLocal lymphocytesOrgan dysfunctionDendritic cellsImmune cellsT cellsEpithelial cellsTissue integrityCellsSurveillanceBody surfaceImmunopathologyImmunoregulationDysfunctionLymphocytesInfectionAntigenImmunoprotectionStress response
2008
Skint1, the prototype of a newly identified immunoglobulin superfamily gene cluster, positively selects epidermal γδ T cells
Boyden LM, Lewis JM, Barbee SD, Bas A, Girardi M, Hayday AC, Tigelaar RE, Lifton RP. Skint1, the prototype of a newly identified immunoglobulin superfamily gene cluster, positively selects epidermal γδ T cells. Nature Genetics 2008, 40: 656-662. PMID: 18408721, PMCID: PMC4167720, DOI: 10.1038/ng.108.Peer-Reviewed Original Research
2007
Characterizing tumor-promoting T cells in chemically induced cutaneous carcinogenesis
Roberts SJ, Ng BY, Filler RB, Lewis J, Glusac EJ, Hayday AC, Tigelaar RE, Girardi M. Characterizing tumor-promoting T cells in chemically induced cutaneous carcinogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 6770-6775. PMID: 17412837, PMCID: PMC1871860, DOI: 10.1073/pnas.0604982104.Peer-Reviewed Original ResearchConceptsT cell deficiencyT cellsCell deficiencyPRO cellsCell-deficient miceTumor-infiltrating lymphocytesAnti-tumor responseT cell populationsInflammation-associated carcinogenesisT cell receptorImmunotherapeutic strategiesRegulatory cellsCancer immunosurveillanceCell reconstitutionEpidemiologic linkCutaneous carcinogenesisTumor incidenceCyclooxygenase-2Activated populationCell receptorNovel populationCell populationsChemical carcinogenesisCarcinogenesisLymphocytes
2005
Sustained localized expression of ligand for the activating NKG2D receptor impairs natural cytotoxicity in vivo and reduces tumor immunosurveillance
Oppenheim DE, Roberts SJ, Clarke SL, Filler R, Lewis JM, Tigelaar RE, Girardi M, Hayday AC. Sustained localized expression of ligand for the activating NKG2D receptor impairs natural cytotoxicity in vivo and reduces tumor immunosurveillance. Nature Immunology 2005, 6: 928-937. PMID: 16116470, DOI: 10.1038/ni1239.Peer-Reviewed Original ResearchMeSH Keywords9,10-Dimethyl-1,2-benzanthraceneAnimalsCarcinomaCell Line, TumorDisease SusceptibilityDown-RegulationFemaleImmunologic SurveillanceKiller Cells, NaturalLigandsMaleMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicNK Cell Lectin-Like Receptor Subfamily KPapillomaReceptors, ImmunologicReceptors, Natural Killer CellSkin NeoplasmsTetradecanoylphorbol AcetateT-LymphocytesTumor BurdenConceptsNKG2D downregulationNK cell-mediated cytotoxicityNatural killer cellsCell-mediated cytotoxicityInnate immune activationT cell defectsNKG2D engagementNatural cytotoxicityKiller cellsImmune activationReceptor NKG2DTumor immunosurveillanceCutaneous carcinogenesisTumor surveillanceT cellsReversible defectsRAE-1Normal epitheliumLigand expressionTumor resistanceCell defectsSustained expressionNKG2DImmunosurveillanceDownregulationThe Integration of Conventional and Unconventional T Cells that Characterizes Cell‐Mediated Responses
Pennington D, Vermijlen D, Wise E, Clarke S, Tigelaar R, Hayday A. The Integration of Conventional and Unconventional T Cells that Characterizes Cell‐Mediated Responses. Advances In Immunology 2005, 87: 27-59. PMID: 16102571, DOI: 10.1016/s0065-2776(05)87002-6.Peer-Reviewed Original ResearchConceptsUnconventional T cellsGene expression analysisConventional T cellsExtensive gene expression analysisT cellsExpression analysisRegulatory functionsCell-mediated immune responsesCell-mediated responsesT cell activityAntigen-specific responsesCellsInflammatory diseasesImmune responseImmunopathology
2003
Subset-specific, uniform activation among Vγ6/Vδ1+ γδ T cells elicited by inflammation
Roark C, Aydintug M, Lewis J, Yin X, Lahn M, Hahn Y, Born W, Tigelaar R, O’Brien R. Subset-specific, uniform activation among Vγ6/Vδ1+ γδ T cells elicited by inflammation. Journal Of Leukocyte Biology 2003, 75: 68-75. PMID: 14525969, DOI: 10.1189/jlb.0703326.Peer-Reviewed Original ResearchConceptsGamma delta T cell subsetT cell subsetsT cell receptorCell subsetsDelta antibodyActivation/memory phenotypeInvariant T cell receptorΓδ T cellsMemory phenotypeInflammatory responseT cellsFlow cytometryCell receptorInflammationMonoclonal antibodiesAntibodiesSelect tissuesCellsTissueSubsetVδ1ThymusThe inter-relatedness and interdependence of mouse T cell receptor γδ+ and αβ+ cells
Pennington D, Silva-Santos B, Shires J, Theodoridis E, Pollitt C, Wise E, Tigelaar R, Owen M, Hayday A. The inter-relatedness and interdependence of mouse T cell receptor γδ+ and αβ+ cells. Nature Immunology 2003, 4: 991-998. PMID: 14502287, DOI: 10.1038/ni979.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCyclic AMP Response Element ModulatorDNA-Binding ProteinsFlow CytometryGene Expression ProfilingGenes, T-Cell ReceptorH-Y AntigenMaleMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicReceptors, Antigen, T-Cell, alpha-betaReceptors, Antigen, T-Cell, gamma-deltaRepressor ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerT-LymphocytesConceptsΑβ T cellsConventional αβ T cellsCell developmentFunctional potentialGene profilesT cell receptorT cellsMouse T-cell receptorΓδ T cell developmentT cell developmentT cell progenitorsCell receptorGene expressionCell progenitorsCell populationsImmune responseTCR specificityCellsReceptorsPotential interdependenceProgenitorsExpression
2001
Regulation of Cutaneous Malignancy by γδ T Cells
Girardi M, Oppenheim D, Steele C, Lewis J, Glusac E, Filler R, Hobby P, Sutton B, Tigelaar R, Hayday A. Regulation of Cutaneous Malignancy by γδ T Cells. Science 2001, 294: 605-609. PMID: 11567106, DOI: 10.1126/science.1063916.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarcinogensCell LineCytotoxicity, ImmunologicDimerizationEpidermisEpithelial CellsHistocompatibility Antigens Class IHumansImmunologic SurveillanceLigandsMembrane ProteinsMiceMice, Inbred C57BLMinor Histocompatibility AntigensMolecular Sequence DataNK Cell Lectin-Like Receptor Subfamily KProtein ConformationProtein FoldingReceptors, Antigen, T-Cell, alpha-betaReceptors, Antigen, T-Cell, gamma-deltaReceptors, ImmunologicReceptors, Natural Killer CellRecombinant Fusion ProteinsReverse Transcriptase Polymerase Chain ReactionSkin NeoplasmsT-Lymphocyte SubsetsConceptsT cellsGammadelta cellsLocal T cellsNatural killer cellsΓδ T cellsGammadelta T cellsCytolytic T cellsSkin carcinoma cellsNKG2D engagementMultiple regimensKiller cellsCutaneous malignanciesCutaneous carcinogenesisEpithelial malignanciesRAE-1Human MICAMalignancyCarcinoma cellsSkin cellsCellsNKG2DRegimensMiceEpitheliumCarcinogenesis
2000
Development of Dendritic Epidermal T Cells with a Skewed Diversity of γδTCRs in Vδ1-Deficient Mice
Hara H, Kishihara K, Matsuzaki G, Takimoto H, Tsukiyama T, Tigelaar R, Nomoto K. Development of Dendritic Epidermal T Cells with a Skewed Diversity of γδTCRs in Vδ1-Deficient Mice. The Journal Of Immunology 2000, 165: 3695-3705. PMID: 11034374, DOI: 10.4049/jimmunol.165.7.3695.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalCell DifferentiationClone CellsCytokinesDendritic CellsEmbryonic and Fetal DevelopmentEpidermal CellsEpidermisFemaleFibroblast Growth Factor 10Fibroblast Growth Factor 7Fibroblast Growth FactorsGene DeletionGene Rearrangement, delta-Chain T-Cell Antigen ReceptorGenes, T-Cell Receptor deltaGenetic MarkersGrowth SubstancesImmunophenotypingMaleMiceMice, Inbred C57BLMice, KnockoutProtein ConformationReceptors, Antigen, T-Cell, gamma-deltaStem CellsT-LymphocytesThymus GlandGranulocyte-Macrophage Colony-Stimulating Factor Priming plus Papillomavirus E6 DNA Vaccination: Effects on Papilloma Formation and Regression in the Cottontail Rabbit Papillomavirus-Rabbit Model
Leachman S, Tigelaar R, Shlyankevich M, Slade M, Irwin M, Chang E, Wu T, Xiao W, Pazhani S, Zelterman D, Brandsma J. Granulocyte-Macrophage Colony-Stimulating Factor Priming plus Papillomavirus E6 DNA Vaccination: Effects on Papilloma Formation and Regression in the Cottontail Rabbit Papillomavirus-Rabbit Model. Journal Of Virology 2000, 74: 8700-8708. PMID: 10954571, PMCID: PMC116381, DOI: 10.1128/jvi.74.18.8700-8708.2000.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiopsyCottontail rabbit papillomavirusDisease Models, AnimalDisease-Free SurvivalDNA, ViralGene Transfer TechniquesGenetic VectorsGranulocyte-Macrophage Colony-Stimulating FactorImmunohistochemistryIn Situ HybridizationPapillomaPapillomavirus InfectionsRabbitsTumor Virus InfectionsVaccines, DNAViral VaccinesConceptsGM-CSF primingGM-CSF treatmentDNA vaccinationDNA inoculationPapilloma formationGM-CSFProfessional antigen-presenting cellsMajor histocompatibility complex classDisease-free survivalVaccination-challenge experimentsAntigen-presenting cellsII-positive cellsGranulocyte-macrophage colony-stimulating factorHistocompatibility complex classColony-stimulating factorGranulocyte-macrophage colonyPapilloma volumeVaccination regimenClinical outcomesCutaneous sitesDNA vaccineViral challengePapilloma growthControl rabbitsCombination treatmentEnterocyte Expression of Interleukin 7 Induces Development of γδ T Cells and Peyer's Patches
Laky K, Lefrançois L, Lingenheld E, Ishikawa H, Lewis J, Olson S, Suzuki K, Tigelaar R, Puddington L. Enterocyte Expression of Interleukin 7 Induces Development of γδ T Cells and Peyer's Patches. Journal Of Experimental Medicine 2000, 191: 1569-1580. PMID: 10790431, PMCID: PMC2213426, DOI: 10.1084/jem.191.9.1569.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsDendritic CellsEnterocytesEpidermal CellsEpidermisFatty Acid-Binding Protein 7Fatty Acid-Binding ProteinsGerminal CenterInterleukin-7Intestine, SmallMiceMice, TransgenicMyelin P2 ProteinNeoplasm ProteinsNerve Tissue ProteinsPeyer's PatchesReceptors, Antigen, T-Cell, gamma-deltaRecombinant Fusion ProteinsT-LymphocytesTissue DistributionTransgenesConceptsTCR-gammadelta cellsIntraepithelial lymphocytesT cell developmentCell developmentPeyer's patchesIL-7Extrathymic T cell developmentΓδ T cellsMucosal lymphoid tissuesGammadelta intraepithelial lymphocytesIL-7 expressionT cell receptorB cell maturationIntestinal fatty acidExtrathymic developmentLymphoid tissueT cellsIntestinal mucosaEnterocyte expressionBone marrowProtein promoterIntestinal epitheliumCell maturationCell receptorMiceTh2 responses induced by epicutaneous or inhalational protein exposure are differentially dependent on IL-4
Herrick C, MacLeod H, Glusac E, Tigelaar R, Bottomly K. Th2 responses induced by epicutaneous or inhalational protein exposure are differentially dependent on IL-4. Journal Of Clinical Investigation 2000, 105: 765-775. PMID: 10727445, PMCID: PMC377464, DOI: 10.1172/jci8624.Peer-Reviewed Original ResearchConceptsTh2 responsesIL-13IL-4Th2-type immune responseIL-4-deficient miceTh2-promoting cytokinesOVA-specific IgG1Bronchoalveolar lavage fluidWild-type miceAllergen encounterTh2 sensitizationAirway challengeTh2 activationAllergen exposureAtopic individualsBAL fluidEnvironmental allergensWT miceLavage fluidPrimary exposureVivo depletionAnatomic sitesImmune responseAnimal modelsMice
1998
Intracutaneous vaccination of rabbits with the E6 gene of cottontail rabbit papillomavirus provides partial protection against virus challenge
Sundaram P, Tigelaar R, Xiao W, Brandsma J. Intracutaneous vaccination of rabbits with the E6 gene of cottontail rabbit papillomavirus provides partial protection against virus challenge. Vaccine 1998, 16: 613-623. PMID: 9569473, DOI: 10.1016/s0264-410x(97)84510-0.Peer-Reviewed Original ResearchConceptsCottontail rabbit papillomavirusHumoral immunityControl rabbitsIntracutaneous vaccinationChallenge sitePeripheral blood mononuclear cellsE6 genePartial protectionCRPV E6 proteinsRabbit papillomavirusBlood mononuclear cellsEffect of vaccinationPapillomavirus-induced diseaseE6 proteinMicrograms DNAPartial prophylaxisBooster vaccinationControl vaccineInitial vaccinationProphylactic immunityCellular immunityMononuclear cellsVirus challengeDNA vaccinationPapilloma burdenConservation of T Cell Receptor Conformation in Epidermal γδ Cells with Disrupted Primary Vγ Gene Usage
Mallick-Wood C, Lewis J, Richie L, Owen M, Tigelaar R, Hayday A. Conservation of T Cell Receptor Conformation in Epidermal γδ Cells with Disrupted Primary Vγ Gene Usage. Science 1998, 279: 1729-1733. PMID: 9497293, DOI: 10.1126/science.279.5357.1729.Peer-Reviewed Original ResearchConceptsGene segmentsVariable region gene segmentsRegion gene segmentsParticular gene segmentsReceptor conformationTCR chainsReceptor gammaAssociation of expressionAlphabeta T cellsCellsConservationT-cell receptor gammaConformational determinantsGammadelta T cell subsetsGene usageT cellsConformationΓδ cellsB cellsLymphocyte repertoireVgamma5SitesDendritic epidermal T cellsExpressionRepertoire
1997
Intracutaneous vaccination of rabbits with the cottontail rabbit papillomavirus (CRPV) L1 gene protects against virus challenge
Sundaram P, Tigelaar R, Brandsma J. Intracutaneous vaccination of rabbits with the cottontail rabbit papillomavirus (CRPV) L1 gene protects against virus challenge. Vaccine 1997, 15: 664-671. PMID: 9178468, DOI: 10.1016/s0264-410x(96)00237-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ViralAntigens, ViralBiolisticsCell DivisionCHO CellsCloning, MolecularCottontail rabbit papillomavirusCricetinaeEpitopes, T-LymphocyteInjections, SubcutaneousNeutralization TestsPapillomavirus InfectionsRabbitsT-LymphocytesTumor Virus InfectionsVaccines, SyntheticViral Structural ProteinsViral VaccinesConceptsCottontail rabbit papillomavirusHigh titered antibodiesVaccination of rabbitsCellular proliferative responseMajor capsid protein L1Capsid protein L1Papillomavirus infectionTitered antibodiesDNA vaccineVirus challengeProliferative responseIntracutaneous vaccinationPapilloma formationRabbit papillomavirusVirus-like particlesL1 geneL1 DNARabbitsVaccinationAntibodies
1996
Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase.
Mallick-Wood C, Pao W, Cheng A, Lewis J, Kulkarni S, Bolen J, Rowley B, Tigelaar R, Pawson T, Hayday A. Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 9704-9709. PMID: 8790395, PMCID: PMC38493, DOI: 10.1073/pnas.93.18.9704.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsElectrophoresis, Polyacrylamide GelEnzyme PrecursorsEpithelial CellsEpitheliumFlow CytometryIntracellular Signaling Peptides and ProteinsLymphocytesMiceMutagenesis, Site-DirectedPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthProtein-Tyrosine KinasesReceptors, Antigen, T-Cell, gamma-deltaSyk KinaseConceptsGamma delta T cellsDelta T cellsIntraepithelial gamma delta T cellsT cellsSplenic gamma delta T cellsSyk tyrosine kinaseGamma delta T cell repertoireGene rearrangementsT cell repertoireReceptor gene rearrangementsSyk-deficient miceDelta gene rearrangementsAntigen receptor gene rearrangementsTyrosine kinaseSyk deficiencyCell repertoireB cellsChimeric miceMiceSpecific epitheliaCellsLymphocytesSubstantial depletionCharacteristic associationsDevelopmental arrest
1995
Thy‐1 + dendritic cells express truncated form of POMC mRNA
Farooqui J, Medrano E, Boissy R, Tigelaar R, Nordlund J. Thy‐1 + dendritic cells express truncated form of POMC mRNA. Experimental Dermatology 1995, 4: 297-301. PMID: 8589920, DOI: 10.1111/j.1600-0625.1995.tb00208.x.Peer-Reviewed Original ResearchConceptsDendritic cellsThy-1Anterior pituitary tumor cell lineACTH precursor proopiomelanocortinEpidermal Thy-1Expression of proopiomelanocortinNorthern blot analysisBlot analysisPituitary tumor cell lineDerivative peptidesMucocutaneous disordersThymic lymphocytesPOMC peptidesPrecursor proopiomelanocortinTumor cell linesPOMC mRNAProopiomelanocortinPigmentary anomaliesPositive controlACTHCell linesLymphocytesAtT20 cellsPolyclonal antibodiesTotal RNAImmunobiology of Mouse Dendritic Epidermal T Cells: A Decade Later, Some Answers, But Still More Questions
Tigelaar R, Lewis J. Immunobiology of Mouse Dendritic Epidermal T Cells: A Decade Later, Some Answers, But Still More Questions. Journal Of Investigative Dermatology 1995, 105: s43-s49. DOI: 10.1038/jid.1995.9.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDendritic epidermal T cellsEpidermal T cellsT cell receptorΓδ cellsT cellsMouse dendritic epidermal T-cellsMurine dendritic epidermal T cellsCutaneous immune surveillanceNormal human skinSelf antigensImmune surveillanceNormal miceActive hair growthAdult miceEpithelial sitesCutaneous physiologyReproductive tractTissue restrictionHair growthFunctional activityMiceSkinHuman skinReceptorsOverwhelming evidenceMurine epidermal V gamma 5/V delta 1-T-cell receptor+ T cells respond to B-cell lines and lipopolysaccharides.
Reardon C, Heyborne K, Tsuji M, Zavala F, Tigelaar R, O'Brien R, Born W. Murine epidermal V gamma 5/V delta 1-T-cell receptor+ T cells respond to B-cell lines and lipopolysaccharides. Journal Of Investigative Dermatology 1995, 105: 58s-61s. PMID: 7615998, DOI: 10.1111/1523-1747.ep12316074.Peer-Reviewed Original ResearchConceptsB cell linesColony-stimulating factorCytokine productionGamma deltaGamma delta T cell linesGranulocyte-macrophage colony-stimulating factorT cell clonesB-cell lymphomaAbsence of lipopolysaccharideT cell stimulationMacrophage colony-stimulating factorCell linesT cell receptorT cell linesIL-10C57BL/6 miceB lymphoma cell lineIL-6IL-8IL-2IL-4IL-5Tumor necrosisT cellsHuman B lymphoma cell lineImmunobiology of mouse dendritic epidermal T cells: a decade later, some answers, but still more questions.
Tigelaar R, Lewis J. Immunobiology of mouse dendritic epidermal T cells: a decade later, some answers, but still more questions. Journal Of Investigative Dermatology 1995, 105: 43s-49s. PMID: 7615996, DOI: 10.1111/1523-1747.ep12315280.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDendritic epidermal T cellsEpidermal T cellsGamma delta cellsT cell receptorT cellsMouse dendritic epidermal T-cellsMurine dendritic epidermal T cellsDelta T-cell receptorCutaneous immune surveillanceDelta cellsAlpha beta cellsNormal human skinSelf antigensImmune surveillanceNormal miceActive hair growthAdult miceBeta cellsEpithelial sitesCutaneous physiologyReproductive tractTissue restrictionHair growthFunctional activityMice