1999
Transduction of Human Progenitor Hematopoietic Stem Cells by Human Immunodeficiency Virus Type 1-Based Vectors Is Cell Cycle Dependent
Sutton R, Reitsma M, Uchida N, Brown P. Transduction of Human Progenitor Hematopoietic Stem Cells by Human Immunodeficiency Virus Type 1-Based Vectors Is Cell Cycle Dependent. Journal Of Virology 1999, 73: 3649-3660. PMID: 10196257, PMCID: PMC104140, DOI: 10.1128/jvi.73.5.3649-3660.1999.Peer-Reviewed Original ResearchConceptsProgenitor hematopoietic stem cellsHematopoietic stem cellsCell cycleG0 cellsStem cellsTransduction ratesG2/M phaseReplication intermediatesCellular factorsHuman immunodeficiency virus type 1 vectorsTransduction efficiencyTransductionAddition of cytokinesM phaseHuman immunodeficiency virus type 1Immunodeficiency virus type 1Presence of cytokinesLentivirus vectorFurther characterizationReverse transcriptionDistinct subpopulationsG2/M fractionVirus type 1CellsTime of initiation
1998
HIV, but not murine leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells
Uchida N, Sutton R, Friera A, He D, Reitsma M, Chang W, Veres G, Scollay R, Weissman I. HIV, but not murine leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 11939-11944. PMID: 9751769, PMCID: PMC21744, DOI: 10.1073/pnas.95.20.11939.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CD34Base SequenceColony-Forming Units AssayDNA PrimersG1 PhaseGene ExpressionGene Transfer TechniquesGenes, ReporterGenetic TherapyGenetic VectorsGreen Fluorescent ProteinsHematopoietic Stem CellsHIV-1HumansIn Vitro TechniquesLeukemia Virus, MurineLuminescent ProteinsPhenotypeResting Phase, Cell CycleThy-1 AntigensTransduction, GeneticConceptsTransgene expressionHigh-efficiency gene transferSingle-step transductionGene delivery systemsGreen fluorescent proteinHematopoietic stem cellsHuman HSC subsetsVector supernatantsEfficient transductionTransduction efficiencyStem cellsHuman hematopoietic stem cellsMurine leukemia virusHIV vectorsDelivery systemGene transferGFP expressionHuman cell typesStem cell phenotypeReplication-defective HIVBone marrow stromal cellsLeukemia virusFluorescent proteinMarrow stromal cellsG0/G1Human Immunodeficiency Virus Type 1 Vectors Efficiently Transduce Human Hematopoietic Stem Cells
Sutton R, Wu H, Rigg R, Böhnlein E, Brown P. Human Immunodeficiency Virus Type 1 Vectors Efficiently Transduce Human Hematopoietic Stem Cells. Journal Of Virology 1998, 72: 5781-5788. PMID: 9621037, PMCID: PMC110379, DOI: 10.1128/jvi.72.7.5781-5788.1998.Peer-Reviewed Original ResearchConceptsHuman hematopoietic stem cellsHematopoietic stem cellsStem cellsMarker gene expressionLentivirus vector systemGene transfer agentsMarker gene productLong-term persistenceGene productsGene expressionHuman immunodeficiency virus type 1 vectorsIntegrated provirusStable expressionVector systemTransduction ratesCellsExpressionHuman immunodeficiency virus type 1Immunodeficiency virus type 1Virus type 1MitosisTransductionDeletionOncoretrovirusesVpr