Endothelial-targeted Gene Transfer of Hypoxia-inducible Factor-1α Augments Ischemic Neovascularization Following Systemic Administration
Tal R, Shaish A, Rofe K, Feige E, Varda-Bloom N, Afek A, Barshack I, Bangio L, Hodish I, Greenberger S, Peled M, Breitbart E, Harats D. Endothelial-targeted Gene Transfer of Hypoxia-inducible Factor-1α Augments Ischemic Neovascularization Following Systemic Administration. Molecular Therapy 2008, 16: 1927-1936. PMID: 28189008, DOI: 10.1038/mt.2008.191.Peer-Reviewed Original ResearchTherapeutic efficacyProangiogenic gene therapyHIF-1aMurine preproendothelin-1 promoterTail vein administrationPreproendothelin-1 promoterSystemic side effectsLack of therapeutic efficacySystemic IV administrationHypoxia-inducible factor 1aRoute of administrationIncreased capillary densityMouse hindlimb ischemia modelHindlimb ischemia modelDeliver genesEnhanced blood perfusionGene therapyIntraarterial routeC57BL/6 miceClinical outcomesSystemic toxicityIschemic neovascularizationSide effectsBiodistribution studiesIschemic muscleEndothelial-targeted gene transfer of hypoxia-inducible factor-1alpha augments ischemic neovascularization following systemic administration.
Tal R, Shaish A, Rofe K, Feige E, Varda-Bloom N, Afek A, Barshack I, Bangio L, Hodish I, Greenberger S, Peled M, Breitbart E, Harats D. Endothelial-targeted gene transfer of hypoxia-inducible factor-1alpha augments ischemic neovascularization following systemic administration. Molecular Therapy 2008, 16: 1927-36. PMID: 18797451, DOI: 10.1038/mt.2008.191.Peer-Reviewed Original ResearchConceptsHypoxia-inducible factor-1alphaSystemic administrationTherapeutic efficacyProangiogenic gene therapyFactor-1alphaSystemic side effectsEnhanced blood perfusionRoute of administrationTail vein administrationHindlimb ischemia modelMouse hindlimb ischemia modelMurine preproendothelin-1 promoterClinical outcomesPreproendothelin-1 promoterC57BL/6 miceIschemic neovascularizationIntravenous routeIschemic muscleIntraarterial routeIschemia modelCapillary densityIschemic regionSide effectsSystemic toxicityTherapeutic angiogenesis