1990
Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated.
Reaves B, Van Itallie C, Moore H, Dannies P. Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated. Endocrinology 1990, 4: 1017-26. PMID: 2284004, DOI: 10.1210/mend-4-7-1017.Peer-Reviewed Original ResearchConceptsGH4C1 cellsEpidermal growth factorPRL synthesisGrowth factorPreferential increaseCombination of estradiolRegulated pathwayRat pituitary tumor cellsIntracellular PRLPituitary tumor cellsBasal releasePRL releaseIntracellular proinsulinSame time courseHormone treatmentPRL storageTumor cellsProinsulin immunoreactivityMRNA levelsPRLInsulinSame peak heightHormoneProinsulinTreatment
1981
Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*
KIINO D, DANNIES P. Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*. Endocrinology 1981, 109: 1264-1269. PMID: 7026222, DOI: 10.1210/endo-109-4-1264.Peer-Reviewed Original ResearchConceptsIntracellular PRLEffect of insulinDays of treatmentHalf-maximal doseRat pituitary tumor cellsPituitary tumor cellsIntracellular prolactin contentM estradiolEstradiolCombined treatmentPRLInsulinPRL synthesisTumor cellsGH4C1 cellsM insulinControl levelsProlactin contentIntracellular hormoneM. TreatmentTotal proteinTreatmentDoseIntracellular transit timeSmall increase
1980
Action of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*
DANNIES P, TASHJIAN A. Action of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*. Endocrinology 1980, 106: 1532-1536. PMID: 6244936, DOI: 10.1210/endo-106-5-1532.Peer-Reviewed Original ResearchConceptsGH synthesisCholera toxinIntracellular cAMPWeeks of treatmentThyrotropin-releasing hormoneRelease of PRLHormone synthesisRat pituitary cellsIntracellular PRLGrowth hormone synthesisPRL releaseSimilar lag periodPituitary cellsGH cellsControl valuesPRL synthesisTRHPRLToxinClonal strainsLag periodReleaseCellsCAMPDecrease