2008
063Electroporation‐Facilitated Gene Therapy for Wound Healing
Lin M, Marti G, Dieb R, Wang J, Ferguson M, Qaiser R, Bonde P, Duncan M, Harmon J. 063Electroporation‐Facilitated Gene Therapy for Wound Healing. Wound Repair And Regeneration 2008, 13: a4-a27. DOI: 10.1111/j.1067-1927.2005.130215bk.x.Peer-Reviewed Original ResearchGrowth factorWound healingResolution of inflammationNIH image analysis softwareLimited clinical successPlasmid transfectionQuality of healingGene therapyLuciferase vectorPeptide growth factorsCecal ligationHistologic assessmentPunch biopsySprague-DawleyEP scoreClinical successTopical applicationCutaneous woundsDay 12Epithelial coverageDNA expression vectorsWound closureExpression vectorHealingWounds
2004
Electroporative transfection with KGF-1 DNA improves wound healing in a diabetic mouse model
Marti G, Ferguson M, Wang J, Byrnes C, Dieb R, Qaiser R, Bonde P, Duncan M, Harmon J. Electroporative transfection with KGF-1 DNA improves wound healing in a diabetic mouse model. Gene Therapy 2004, 11: 1780-1785. PMID: 15470477, DOI: 10.1038/sj.gt.3302383.Peer-Reviewed Original ResearchConceptsKeratinocyte growth factorDiabetic mouse modelMouse modelWound healing modelExcisional woundsWound closureEpithelial cell proliferationRT-PCR assaysDiabetic miceDiabetic individualsUnresolved inflammationDNA injectionMouse wound-healing modelUntreated groupSingle injectionDay 12KGF mRNAELISA assaysKGF proteinHistological analysisGrowth factorSubsequent electroporationNaked DNA injectionCell proliferationWoundsTransfection with keratinocyte growth factor 1 cDNA with electroporation improves wound healing in an aged mouse model
Marti G, Makary M, Ferguson M, Wang J, Dieb R, Marti A, Lin M, Bonde P, Duncan M, Harmon J. Transfection with keratinocyte growth factor 1 cDNA with electroporation improves wound healing in an aged mouse model. Journal Of Surgical Research 2004, 121: 320-321. DOI: 10.1016/j.jss.2004.07.175.Peer-Reviewed Original ResearchAged animal modelsAnimal modelsWound sizeWound closureAged mouse modelMice 6 weeksKGF-1Young animalsAverage wound sizeT-testStudent's t-testCutaneous wound closureFull-thickness woundsElderly miceAged miceBALBc miceMouse modelAge groupsDay 12DNA expression vectorsGrowth factorMiceWound areaProliferative capacityUntreated woundsKGF-1 plasmid delivered with electroporation accelerates wound closure in diabetic mice
Marti G, Lin M, Qaiser R, Dieb R, Wang J, Parth S, Bonde P, Duncan M, Harmon J. KGF-1 plasmid delivered with electroporation accelerates wound closure in diabetic mice. Journal Of The American College Of Surgeons 2004, 199: 59. DOI: 10.1016/j.jamcollsurg.2004.05.121.Peer-Reviewed Original ResearchDiabetic miceGrowth factorKGF-1Diabetic mouse modelWound healing deficitsUnmet medical challengeKeratinocyte growth factorFull-thickness woundsImportant growth factorDiabetic individualsHistological assessmentHealing deficitsMouse modelEP groupClosure of woundsGrading scaleMedical challengeDNA expression vectorsThickness woundsWound areaDiminished levelsWoundsUntreated woundsMiceCMV promoter