2018
Optical Transfection.
Kumar P, Nagarajan A, Uchil PD. Optical Transfection. Cold Spring Harbor Protocols 2018, 2018: pdb.top096222. PMID: 30510131, DOI: 10.1101/pdb.top096222.Peer-Reviewed Original ResearchAnalysis of Cell Viability by the Lactate Dehydrogenase Assay.
Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the Lactate Dehydrogenase Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095497. PMID: 29858337, DOI: 10.1101/pdb.prot095497.Peer-Reviewed Original ResearchConceptsCytoplasmic enzymePlasma membraneConversion of lactateLactate dehydrogenase assayLactate dehydrogenaseCellular damageYellow tetrazolium saltCell viabilityAmount of LDHDehydrogenase assayEnzymeCellsNADHTetrazolium saltCell culture supernatantsCulture supernatantsLDH activityAmount of formazanApoptosisDehydrogenaseMembraneActivityAssays
2008
Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides
Chan R, Uchil PD, Jin J, Shui G, Ott DE, Mothes W, Wenk MR. Retroviruses Human Immunodeficiency Virus and Murine Leukemia Virus Are Enriched in Phosphoinositides. Journal Of Virology 2008, 82: 11228-11238. PMID: 18799574, PMCID: PMC2573248, DOI: 10.1128/jvi.00981-08.Peer-Reviewed Original ResearchConceptsPlasma membraneMurine leukemia virusHIV-1 buddingLipid contentLeukemia virusMass spectrometry analysisCholesterol-rich regionsOverall lipid contentCell peripheryMutant virionsLipid envelopeTotal lipid contentPhosphorylated derivativesSpectrometry analysisPhosphoinositideRetrovirusesMembraneCellsMatrix domainParticle releaseHuman immunodeficiency virus type 1Immunodeficiency virus type 1VirusVirus type 1Phosphatidylinositol
2005
Viral entry: a detour through multivesicular bodies
Uchil P, Mothes W. Viral entry: a detour through multivesicular bodies. Nature Cell Biology 2005, 7: 641-642. PMID: 15990891, DOI: 10.1038/ncb0705-641.Peer-Reviewed Original Research