2024
Complex trauma sequelae: Mycobacterium goodii and Priestia endophytica Hardware infection in a patient with Ehlers-Danlos syndrome
Covington A, Cerqueira F, Pavia J, Reynoso D, Ren P. Complex trauma sequelae: Mycobacterium goodii and Priestia endophytica Hardware infection in a patient with Ehlers-Danlos syndrome. BMC Infectious Diseases 2024, 24: 1064. PMID: 39333951, PMCID: PMC11438204, DOI: 10.1186/s12879-024-09970-1.Peer-Reviewed Original ResearchConceptsEhlers-Danlos syndromeSevere left leg painMycobacterium goodiiChronic otitis mediaOpen femur fracturesRecurrent otitis externaDaptomycin treatmentGram-positive bacilliHardware infectionLeg painImmunocompromised patientsPurulent dischargeMotor vehicle collisionsOtitis mediaOrthopedic complicationsOtitis externaHardware fixationFemur fracturesOpportunistic human pathogenEmergency departmentLower extremity amputationPatientsSyndromeHuman pathogensInfection
2023
Function and Cryo-EM structures of broadly potent bispecific antibodies against multiple SARS-CoV-2 Omicron sublineages
Ren P, Hu Y, Peng L, Yang L, Suzuki K, Fang Z, Bai M, Zhou L, Feng Y, Zou Y, Xiong Y, Chen S. Function and Cryo-EM structures of broadly potent bispecific antibodies against multiple SARS-CoV-2 Omicron sublineages. Signal Transduction And Targeted Therapy 2023, 8: 281. PMID: 37518189, PMCID: PMC10387464, DOI: 10.1038/s41392-023-01509-1.Peer-Reviewed Original ResearchRAMIHM generates fully human monoclonal antibodies by rapid mRNA immunization of humanized mice and BCR-seq
Ren P, Peng L, Yang L, Suzuki K, Fang Z, Renauer P, Lin Q, Bai M, Li T, Clark P, Klein D, Chen S. RAMIHM generates fully human monoclonal antibodies by rapid mRNA immunization of humanized mice and BCR-seq. Cell Chemical Biology 2023, 30: 85-96.e6. PMID: 36640761, PMCID: PMC9868106, DOI: 10.1016/j.chembiol.2022.12.005.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyHumanized miceMonoclonal antibodiesMemory B cell populationsHumanized transgenic miceBroad antibody responseB cell populationsG protein-coupled receptor targetsNeutralizing antibodiesPeripheral bloodAntibody responseImmunotherapy targetClinical vaccinesPlasma BCell sequencingTransgenic miceImmunization methodReceptor targetsAntibodiesMiceCell populationsHigh potencyImmunizationHigh rateAntibody discovery
2022
LRRC15 inhibits SARS-CoV-2 cellular entry in trans
Song J, Chow RD, Peña-Hernández MA, Zhang L, Loeb SA, So EY, Liang OD, Ren P, Chen S, Wilen CB, Lee S. LRRC15 inhibits SARS-CoV-2 cellular entry in trans. PLOS Biology 2022, 20: e3001805. PMID: 36228039, PMCID: PMC9595563, DOI: 10.1371/journal.pbio.3001805.Peer-Reviewed Original ResearchConceptsExpression of LRRC15Receptor-binding domainViral entryAcute respiratory syndrome coronavirus 2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionSARS-CoV-2 cellular entrySyndrome coronavirus 2 infectionSARS-CoV-2 entrySpike-mediated entryCoronavirus 2 infectionCOVID-19 patientsCellular entry factorsSARS-CoV-2Attachment factorsACE2-negative cellsEnzyme 2Receptor angiotensinEntry factorsProtective roleLRRC15Spike proteinSame cell typeCRISPR activation screensACE2Cellular entryHeterotypic vaccination responses against SARS-CoV-2 Omicron BA.2
Fang Z, Peng L, Lucas C, Lin Q, Zhou L, Yang L, Feng Y, Ren P, Renauer PA, Monteiro VS, Hahn AM, Park JJ, Zhou X, Grubaugh N, Wilen C, Chen S. Heterotypic vaccination responses against SARS-CoV-2 Omicron BA.2. Cell Discovery 2022, 8: 69. PMID: 35853867, PMCID: PMC9295082, DOI: 10.1038/s41421-022-00435-w.Peer-Reviewed Original ResearchDevelopment of an efficient reproducible cell-cell transmission assay for rapid quantification of SARS-CoV-2 spike interaction with hACE2
Ssenyange G, Kerfoot M, Zhao M, Farhadian S, Chen S, Peng L, Ren P, Dela Cruz CS, Gupta S, Sutton RE. Development of an efficient reproducible cell-cell transmission assay for rapid quantification of SARS-CoV-2 spike interaction with hACE2. Cell Reports Methods 2022, 2: 100252. PMID: 35757815, PMCID: PMC9213030, DOI: 10.1016/j.crmeth.2022.100252.Peer-Reviewed Original ResearchConceptsAnti-spike monoclonal antibodiesTransmission assaysTherapeutic antiviral drugsSARS-CoV-2Quantitative readoutVirus-cell bindingRapid quantificationConvalescent seraNeutralization assaysAntiviral drugsResearch reagentsSmall molecule drugsClinical settingViral replicationPseudotyped particlesMonoclonal antibodiesLaboratory equipmentQuantitative assayOmicron-specific mRNA vaccination alone and as a heterologous booster against SARS-CoV-2
Fang Z, Peng L, Filler R, Suzuki K, McNamara A, Lin Q, Renauer PA, Yang L, Menasche B, Sanchez A, Ren P, Xiong Q, Strine M, Clark P, Lin C, Ko AI, Grubaugh ND, Wilen CB, Chen S. Omicron-specific mRNA vaccination alone and as a heterologous booster against SARS-CoV-2. Nature Communications 2022, 13: 3250. PMID: 35668119, PMCID: PMC9169595, DOI: 10.1038/s41467-022-30878-4.Peer-Reviewed Original ResearchConceptsHeterologous boosterSARS-CoV-2Antibody responseMRNA vaccinesMRNA vaccinationDelta variantOmicron variantType of vaccinationStrong antibody responseMRNA vaccine candidatesVaccine candidatesNeutralization potencyImmune evasionSARS-CoV.Two weeksComparable titersVaccinationVaccineTiters 10MiceOmicronWeeksWA-1LNP-mRNABoosterCAR‐T Therapy in Clinical Practice: Technical Advances and Current Challenges
Ren P, Zhang C, Li W, Wang X, Liang A, Yang G, Xu H, Ma P. CAR‐T Therapy in Clinical Practice: Technical Advances and Current Challenges. Advanced Biology 2022, 6: e2101262. PMID: 35652169, DOI: 10.1002/adbi.202101262.Peer-Reviewed Original ResearchConceptsChimeric antigen receptorCAR T cellsCAR-T therapyClinical trialsCAR T-cell therapyAcute lymphoblastic leukemiaSuccessful clinical outcomeB-cell lymphomaT cell engineeringStandard tumor therapiesAntigen recognition domainComplete responseClinical outcomesTumor toxicityLymphoblastic leukemiaT cellsCell lymphomaClinical experiencePoor efficacyClinical practiceSolid tumorsTumor resistanceTherapyAntitumor efficacyCell therapyMonospecific and bispecific monoclonal SARS-CoV-2 neutralizing antibodies that maintain potency against B.1.617
Peng L, Hu Y, Mankowski MC, Ren P, Chen RE, Wei J, Zhao M, Li T, Tripler T, Ye L, Chow RD, Fang Z, Wu C, Dong MB, Cook M, Wang G, Clark P, Nelson B, Klein D, Sutton R, Diamond MS, Wilen CB, Xiong Y, Chen S. Monospecific and bispecific monoclonal SARS-CoV-2 neutralizing antibodies that maintain potency against B.1.617. Nature Communications 2022, 13: 1638. PMID: 35347138, PMCID: PMC8960874, DOI: 10.1038/s41467-022-29288-3.Peer-Reviewed Original ResearchConceptsSARS-CoV-2Authentic SARS-CoV-2Effective therapeutic optionPotent SARS-CoV-2SARS-CoV-2 variantsVariants of concernRepertoire of therapeuticsBreakthrough infectionsTherapeutic optionsMultiple vaccinesPathogen SARS-CoV-2Delta variantB cellsPotent efficacyHumanized antibodyDistinct epitopesBispecific antibodiesOriginal virusSpike receptorStrong inhibitory activityMonoclonal antibodiesAntibodiesStrong potencyLead clonesLead antibodies
2021
Avidity‐Based Selection of Tissue‐Specific CAR‐T Cells from a Combinatorial Cellular Library of CARs
Ma P, Ren P, Zhang C, Tang J, Yu Z, Zhu X, Fan K, Li G, Zhu W, Sang W, Min C, Chen W, Huang X, Yang G, Lerner RA. Avidity‐Based Selection of Tissue‐Specific CAR‐T Cells from a Combinatorial Cellular Library of CARs. Advanced Science 2021, 8: 2003091. PMID: 33747727, PMCID: PMC7967050, DOI: 10.1002/advs.202003091.Peer-Reviewed Original Research
2019
An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast
Jia Y, Ren P, Duan S, Zeng P, Xie D, Zeng F. An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast. Biotechnology Letters 2019, 41: 1067-1076. PMID: 31300936, DOI: 10.1007/s10529-019-02710-5.Peer-Reviewed Original Research
2017
Cdk1‐interacting protein Cip1 is regulated by the S phase checkpoint in response to genotoxic stress
Zhang Z, Ren P, Vashisht AA, Wohlschlegel JA, Quintana DG, Zeng F. Cdk1‐interacting protein Cip1 is regulated by the S phase checkpoint in response to genotoxic stress. Genes To Cells 2017, 22: 850-860. PMID: 28771906, PMCID: PMC5643225, DOI: 10.1111/gtc.12518.Peer-Reviewed Original ResearchConceptsS-phase checkpointGenotoxic stressPhase checkpointG1 cyclin Cln2Rad53-dependent mannerCDK1 inhibitorS-phase checkpoint responseYeast Saccharomyces cerevisiaeMec1-dependent mannerATM/ATRDe novo protein synthesisCell cycle progressionDependent kinase inhibitorNovo protein synthesisTQ motifsChromosome replicationEukaryotic cellsReplication stressCheckpoint responseChromosomal replicationDownstream kinasesSaccharomyces cerevisiaeGenomic instabilityCycle progressionG1 cyclins
2016
Identification of YPL014W (Cip1) as a novel negative regulator of cyclin‐dependent kinase in Saccharomyces cerevisiae
Ren P, Malik A, Zeng F. Identification of YPL014W (Cip1) as a novel negative regulator of cyclin‐dependent kinase in Saccharomyces cerevisiae. Genes To Cells 2016, 21: 543-552. PMID: 27005485, DOI: 10.1111/gtc.12361.Peer-Reviewed Original ResearchConceptsCyclin-dependent kinasesNovel negative regulatorNegative regulatorCell division cycle progressionCycle progressionCell cycleModel eukaryotic organism SaccharomycesSingle cyclin-dependent kinaseCdk1-dependent mannerWild-type cellsBlocks cell cycle progressionCell cycle progressionClb5-Cdk1Organism SaccharomycesEukaryotic cellsGenomic instabilityUnscheduled proliferationKinaseCIP1SaccharomycesCDK1RegulatorSic1CellsCerevisiae
2015
G1 cyclin driven DNA replication
Palou R, Malik A, Palou G, Zeng F, Ren P, Quintana DG. G1 cyclin driven DNA replication. Cell Cycle 2015, 14: 3842-3850. PMID: 26176277, PMCID: PMC4825731, DOI: 10.1080/15384101.2015.1070995.Peer-Reviewed Original ResearchConceptsCyclin-dependent kinasesDNA replicationPhase cyclinsCycle eventsCell cycleCDK catalytic subunitPhase-specific transcriptionIntrinsic substrate specificityMitotic cell cycleCell cycle eventsS phase cyclinsG1 phase cyclinsChromosome segregationSpecific transcriptionCDK activationCatalytic subunitCDK activitySubstrate specificityG1 cyclinsCyclinSuch regulationSubunitsReplicationHigh enough levelsQuantitative controlElevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome
Gu Urban GJ, Friedman M, Ren P, Törn C, Fex M, Hampe CS, Lernmark Å, Landegren U, Kamali-Moghaddam M. Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome. Scientific Reports 2015, 5: 11196. PMID: 26080009, PMCID: PMC4468815, DOI: 10.1038/srep11196.Peer-Reviewed Original ResearchConceptsStiff-person syndromeImmune complexesPerson syndromeGlutamic acid decarboxylase 65Type 1 diabetesProximity ligation assayAutoimmune diseasesHealthy controlsSP patientsBlood samplesLigation assayClinical diagnosisPg/GAD65Serum samplesImproved diagnosisSyndromeSpecific assayDiseaseDiagnosisPresent studyGADAAssays