2000
High-Efficiency, Long-Term Cardiac Expression of Foreign Genes in Living Mouse Embryos and Neonates
Christensen G, Minamisawa S, Gruber P, Wang Y, Chien K. High-Efficiency, Long-Term Cardiac Expression of Foreign Genes in Living Mouse Embryos and Neonates. Circulation 2000, 101: 178-184. PMID: 10637206, DOI: 10.1161/01.cir.101.2.178.Peer-Reviewed Original ResearchConceptsMouse myocardiumDirect intracardiac injectionAdult mouse myocardiumCardiac gene transferNeonatal miceIntracardiac injectionInfected myocytesMicrosurgical approachFunctional assessmentGene transfer strategiesCardiac expressionVentricular cavityVivo gene transferNormal physiological functionCardiac ventriclesGene transferMurine heartGene expressionPattern of expressionMyocardiumRecombinant adenovirusGene ablationAdenovirus vectorLong-term foreign gene expressionCardiac wall
1998
Downregulation of atrial markers during cardiac chamber morphogenesis is irreversible in murine embryos
Gruber P, Kubalak S, Chien K. Downregulation of atrial markers during cardiac chamber morphogenesis is irreversible in murine embryos. Development 1998, 125: 4427-4438. PMID: 9778502, DOI: 10.1242/dev.125.22.4427.Peer-Reviewed Original ResearchConceptsCardiac chamber morphogenesisChamber morphogenesisMurine embryosChamber specificationMLC-2aOnset of cardiogenesisVentricular chamber maturationMuscle cell lineageWild-type embryosVentricular chamber morphogenesisWild-type backgroundMuscle cellsAtrial markersCritical signaling eventDistinct tissue typesChamber maturationMyosin light chain 2Ventricular muscle cellsVertebrate cardiogenesisLight chain 2Signaling eventsAtrial myosin light chain 2Mechanistic dissectionAlpha-myosin heavy chainCell lineages