2001
Triplex-induced Recombination in Human Cell-free Extracts DEPENDENCE ON XPA AND HsRad51*
Datta H, Chan P, Vasquez K, Gupta R, Glazer P. Triplex-induced Recombination in Human Cell-free Extracts DEPENDENCE ON XPA AND HsRad51*. Journal Of Biological Chemistry 2001, 276: 18018-18023. PMID: 11278954, DOI: 10.1074/jbc.m011646200.Peer-Reviewed Original Research
2000
High-frequency intrachromosomal gene conversion induced by triplex-forming oligonucleotides microinjected into mouse cells
Luo Z, Macris M, Faruqi A, Glazer P. High-frequency intrachromosomal gene conversion induced by triplex-forming oligonucleotides microinjected into mouse cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 9003-9008. PMID: 10900269, PMCID: PMC16811, DOI: 10.1073/pnas.160004997.Peer-Reviewed Original ResearchConceptsTriple helix-forming oligonucleotidesLtk- cell lineTK geneChromosomal lociIntrachromosomal gene conversionMouse Ltk- cell lineSingle chromosomal locusFunctional tk geneGene conversion eventsSite-specific recombinationSequence-specific mannerCell linesSimplex virus thymidine kinase geneVirus thymidine kinase geneHerpes simplex virus thymidine kinase geneThymidine kinase geneGene conversionIdentical base compositionMammalian cellsDownstream genesConversion eventsChromosomal sitesBase compositionKinase geneMutant copies
1999
Peptide nucleic acid (PNA) binding-mediated induction of human γ-globin gene expression
Wang G, Xu X, Pace B, Dean D, Glazer P, Chan P, Goodman S, Shokolenko I. Peptide nucleic acid (PNA) binding-mediated induction of human γ-globin gene expression. Nucleic Acids Research 1999, 27: 2806-2813. PMID: 10373600, PMCID: PMC148492, DOI: 10.1093/nar/27.13.2806.Peer-Reviewed Original ResearchConceptsGamma-globin gene expressionGamma-globin geneD-loop structureGene expressionHuman γ-globin gene expressionΓ-globin gene expressionGenetic diseasesK562 human erythroleukemia cellsGene expression strategyReporter gene constructsSequence-specific mannerBeta-globin geneHuman erythroleukemia cellsInduction of expressionAdult blood cellsEndogenous genesCommon genetic diseaseGene productsGene constructsExpression strategyErythroleukemia cellsHomopurine/homopyrimidine sequencesHuman diseasesGenesGlobin disordersTargeted Correction of an Episomal Gene in Mammalian Cells by a Short DNA Fragment Tethered to a Triplex-forming Oligonucleotide*
Chan P, Lin M, Faruqi A, Powell J, Seidman M, Glazer P. Targeted Correction of an Episomal Gene in Mammalian Cells by a Short DNA Fragment Tethered to a Triplex-forming Oligonucleotide*. Journal Of Biological Chemistry 1999, 274: 11541-11548. PMID: 10206960, DOI: 10.1074/jbc.274.17.11541.Peer-Reviewed Original ResearchConceptsMammalian cellsGene correctionSV40 shuttle vectorTriplex motifNucleotide excision repairSequence-specific mannerEpisomal geneSupF reporter geneXPA cDNASpecific target sitesGene conversionShort DNA fragmentsDNA repairGene targetingTarget genesDNA segmentsReporter geneExcision repairDNA fragmentsBifunctional oligonucleotidesShuttle vectorSequence conversionGenesSV40 vectorsFlexible linker
1995
Altered Repair of Targeted Psoralen Photoadducts in the Context of an Oligonucleotide-mediated Triple Helix (∗)
Wang G, Glazer P. Altered Repair of Targeted Psoralen Photoadducts in the Context of an Oligonucleotide-mediated Triple Helix (∗). Journal Of Biological Chemistry 1995, 270: 22595-22601. PMID: 7673252, DOI: 10.1074/jbc.270.38.22595.Peer-Reviewed Original ResearchConceptsTriplex-forming oligonucleotidesMammalian cellsPattern of mutationsSV40 shuttle vectorHeLa whole cell extractsMonkey COS cellsDNA repair pathwaysNucleotide excision repairSequence-specific mannerWhole cell extractsExcision repair patchesTriple helixPsoralen adductsSite-specific modificationThird strandGenetic manipulationSame target siteRepair pathwaysCOS cellsExcision repairAltered repairShuttle vectorCell extractsIncision stepTarget site
1993
Targeted mutagenesis of simian virus 40 DNA mediated by a triple helix-forming oligonucleotide
Havre P, Glazer P. Targeted mutagenesis of simian virus 40 DNA mediated by a triple helix-forming oligonucleotide. Journal Of Virology 1993, 67: 7324-7331. PMID: 8230456, PMCID: PMC238196, DOI: 10.1128/jvi.67.12.7324-7331.1993.Peer-Reviewed Original ResearchConceptsTriple helix-forming oligonucleotidesTarget genesTranscription factor accessViral genomeTransient expression assaysSimian virus 40 DNASequence-specific mannerDNA sequence analysisGene functionFactor accessMammalian cellsGenetic manipulationHomopurine/homopyrimidineTargeted base pairsPromoter sitesTriple-helical DNAExpression assaysGenetic engineeringGene expressionTargeted mutationsSequence analysisMutagenesisHeritable effectsSV40 DNAGenome