2024
Targeting Pseudomonas aeruginosa biofilm with an evolutionary trained bacteriophage cocktail exploiting phage resistance trade-offs
Kunisch F, Campobasso C, Wagemans J, Yildirim S, Chan B, Schaudinn C, Lavigne R, Turner P, Raschke M, Trampuz A, Gonzalez Moreno M. Targeting Pseudomonas aeruginosa biofilm with an evolutionary trained bacteriophage cocktail exploiting phage resistance trade-offs. Nature Communications 2024, 15: 8572. PMID: 39362854, PMCID: PMC11450229, DOI: 10.1038/s41467-024-52595-w.Peer-Reviewed Original ResearchConceptsResistance trade-offBacteriophage host rangeViruses of bacteriaHuman microbial infectionsMultidrug-resistant bacterial infectionsTwo-phage cocktailMultidrug-resistant Pseudomonas aeruginosa strainsLytic bacteriophagesBiofilm-associatedEvolution assaysPlanktonic culturesBacteriophage cocktailHost rangeBacteriophageHost spectrumBacteriophage therapyCocktail designCombat biofilmsTreated bacteriaMicrobial infectionsAntimicrobial efficacyBacterial suppressionPolymerase chain reactionBacterial infectionsClinical outcomesExperimental Evolution Studies in Φ6 Cystovirus
Singhal S, Balitactac A, Nayagam A, Bahrami P, Nayeem S, Turner P. Experimental Evolution Studies in Φ6 Cystovirus. Viruses 2024, 16: 977. PMID: 38932268, PMCID: PMC11209170, DOI: 10.3390/v16060977.Peer-Reviewed Original ResearchConceptsExperimental evolution studiesFitness effects of spontaneous mutationsSpontaneous mutationsEffects of spontaneous mutationsEvolution of RNA virusesEvolution studiesEvolution of host rangeFast generation timesResponse to selectionMultiple phagesNon-pathogenic modelRNA genomeConsequence of evolutionOuter membraneHost rangeRNA virusesNovel traitsMutation rateHost cellsFitness effectsPopulation sizeMutationsCystovirusesBacteriophageGeneration time
2016
Generalized selection to overcome innate immunity selects for host breadth in an RNA virus
Wasik BR, Muñoz‐Rojas A, Okamoto KW, Miller‐Jensen K, Turner PE. Generalized selection to overcome innate immunity selects for host breadth in an RNA virus. Evolution 2016, 70: 270-281. PMID: 26882316, DOI: 10.1111/evo.12845.Peer-Reviewed Original ResearchConceptsVesicular stomatitis virus populationsInnate immunityRNA virusesHost cell levelVirus-host coevolutionDifferent species originViral fitnessCompetent cellsVSV populationsHost breadthHuman cancer cellsEvolutionary historyFitness differencesHost speciesDisparate hostsSpecies originInfection successInnate immune capacityPrimate cellsInnate immune functionVirus populationsImmune deficient cellsEmergence potentialImmune competent cellsNonhuman primate cells