2023
Phenotypic and Proteomic Characterization of the Human Erythroid Progenitor Continuum Reveal Dynamic Changes in Cell Cycle and in Metabolic Pathways
Papoin J, Yan H, Leduc M, le-Gall M, Narla A, Steiner L, Gallagher P, Hillyer C, Gauthier E, Narla M, Blanc L. Phenotypic and Proteomic Characterization of the Human Erythroid Progenitor Continuum Reveal Dynamic Changes in Cell Cycle and in Metabolic Pathways. Blood 2023, 142: 2455. DOI: 10.1182/blood-2023-189566.Peer-Reviewed Original ResearchCell cycleFaster cycling cellsProgenitor differentiationProgenitor populationsOxidative phosphorylationMass spectrometry-based proteomicsErythroid progenitorsS phaseProtein copy numbersSpectrometry-based proteomicsErythroid progenitor differentiationCycle-related genesG2/MTerminal erythropoiesisProgenitor biologyProteomic characterizationHematopoietic stem cellsE2F membersHuman erythropoiesisHuman bone marrowFunctional analysisErythroid lineageMurine erythropoiesisMetabolic pathwaysS transition
2021
Comprehensive phenotyping of erythropoiesis in human bone marrow: Evaluation of normal and ineffective erythropoiesis
Yan H, Ali A, Blanc L, Narla A, Lane JM, Gao E, Papoin J, Hale J, Hillyer CD, Taylor N, Gallagher PG, Raza A, Kinet S, Mohandas N. Comprehensive phenotyping of erythropoiesis in human bone marrow: Evaluation of normal and ineffective erythropoiesis. American Journal Of Hematology 2021, 96: 1064-1076. PMID: 34021930, PMCID: PMC8355124, DOI: 10.1002/ajh.26247.Peer-Reviewed Original ResearchConceptsTerminal erythroid differentiationErythroid differentiationHuman erythropoiesisErythroid cellsErythroid progenitorsPrimary bone marrow cellsPrimary erythroid cellsDisorders of erythropoiesisStage-specific defectsErythroid progenitor cellsFunctional insightsProgenitor stageProgenitor populationsHuman bone marrowBone marrowFactor responsivenessNormal erythropoiesisProgenitor cellsBone marrow cellsDiscrete populationsColony assayFlow cytometry-based techniqueDifferentiationProliferative capacityEarly population