2009
Genome Wide Profiling of USF1 and USF2 Occupancy in Human Primary Erythroid Cells.
Steiner L, Schulz V, Maksimova Y, Tuck D, Gallagher P. Genome Wide Profiling of USF1 and USF2 Occupancy in Human Primary Erythroid Cells. Blood 2009, 114: 1456. DOI: 10.1182/blood.v114.22.1456.1456.Peer-Reviewed Original ResearchTranscription start siteLocus control regionBeta-globin locus control regionGenome-wide mapsUSF proteinsPrimary erythroid cellsBeta-globin promoterHypersensitive site 2Erythroid cellsTranscription factorsE-boxDevelopmental stage-specific genesInsulator elementsUSF bindingHelix-leucine zipper proteinBeta-globin transcriptionErythroid-expressed genesE-box consensus motifsMammalian erythroid cellsGenome-wide profilingHuman primary erythroid cellsLeucine zipper proteinCanonical E-boxMRNA transcript analysisChIP-seq experimentsA Modified ANK-1 Promoter Directs Uniform, Copy Number Dependent Gamma Globin Gene Expression at Therapeutic Levels of a in a Lentivirus Vector.
Harrow F, Cline A, Seidel N, Persons D, Gallagher P, Bodine D. A Modified ANK-1 Promoter Directs Uniform, Copy Number Dependent Gamma Globin Gene Expression at Therapeutic Levels of a in a Lentivirus Vector. Blood 2009, 114: 3565. DOI: 10.1182/blood.v114.22.3565.3565.Peer-Reviewed Original ResearchBeta-like globin genesMouse alpha-globinGamma-globin geneGamma-globin mRNAGlobin gene expressionLocus control regionGamma-globin expressionGlobin genesAlpha-globinHematopoietic stem cellsGene expressionEnhancer elementsMouse alpha-globin mRNAHuman gamma-globin geneBeta-globin locus control regionGamma-globin gene expressionSequence variantsTranscription initiation complexLCR elementsBeta-like globinCopy number-dependent expressionBinding of TFIIDAlpha-globin mRNACis-acting enhancer elementPromoter sequence variants
2000
A Minimal Ankyrin Promoter Linked to a Human γ-Globin Gene Demonstrates Erythroid Specific Copy Number Dependent Expression with Minimal Position or Enhancer Dependence in Transgenic Mice*
Sabatino D, Wong C, Cline A, Pyle L, Garrett L, Gallagher P, Bodine D. A Minimal Ankyrin Promoter Linked to a Human γ-Globin Gene Demonstrates Erythroid Specific Copy Number Dependent Expression with Minimal Position or Enhancer Dependence in Transgenic Mice*. Journal Of Biological Chemistry 2000, 275: 28549-28554. PMID: 10878017, DOI: 10.1074/jbc.m004043200.Peer-Reviewed Original ResearchConceptsAnkyrin promoterPosition-independent expressionErythroid cellsGlobin genesGlobin mRNAHuman γ-globin genesMouse alpha-globin mRNABeta-globin locus control regionAdult erythroid cellsANK-1 geneΓ-globin geneCopy number-dependent expressionLocus control regionErythroid-specific expressionAlpha-globin mRNAFetal erythroid cellsNumber-dependent expressionGlobin mRNA expressionErythrocyte membrane skeletonTransgene copy numberHS2 enhancerTransgenic linesGlobin transgenesControl regionTransgenic mice