2023
Ventral tegmental area M5 muscarinic receptors mediate effort-choice responding and nucleus accumbens dopamine in a sex-specific manner
Nunes E, Kebede N, Haight J, Foster D, Lindsley C, Conn P, Addy N. Ventral tegmental area M5 muscarinic receptors mediate effort-choice responding and nucleus accumbens dopamine in a sex-specific manner. Journal Of Pharmacology And Experimental Therapeutics 2023, 385: jpet-ar-2022-001438. PMID: 36828630, PMCID: PMC10108441, DOI: 10.1124/jpet.122.001438.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsDopamineFemaleMaleNucleus AccumbensPhysostigmineRatsRats, Sprague-DawleyReceptor, Muscarinic M5Ventral Tegmental AreaConceptsNucleus accumbens coreVentral tegmental areaM5 receptorsDA releaseMale ratsVTA infusionsDepressive disorderNucleus accumbensMuscarinic M5 receptorsSelective negative allosteric modulatorM5 muscarinic receptorsNegative allosteric modulatorsEffort-related choicePhasic DA releasePotential sex differencesSex-specific mannerACh toneVTA administrationCholinergic effectsMuscarinic receptorsFemale ratsTegmental areaPreclinical modelsDopamine releaseTherapeutic target
2020
Examining the role of muscarinic M5 receptors in VTA cholinergic modulation of depressive-like and anxiety-related behaviors in rats
Nunes EJ, Rupprecht LE, Foster DJ, Lindsley CW, Conn PJ, Addy NA. Examining the role of muscarinic M5 receptors in VTA cholinergic modulation of depressive-like and anxiety-related behaviors in rats. Neuropharmacology 2020, 171: 108089. PMID: 32268153, PMCID: PMC7313677, DOI: 10.1016/j.neuropharm.2020.108089.Peer-Reviewed Original ResearchConceptsSucrose preference testVentral tegmental areaMuscarinic M5 receptorsCholinergic toneM5 receptorsBehavioral effectsFemale ratsDepressive-like behavioral effectsIntra-VTA infusionAnxiogenic-like effectsAcetylcholinesterase inhibitor physostigmineAnxiety-related behaviorVTA administrationCholinergic modulationCholinergic receptorsMuscarinic receptorsInhibitor physostigmineSwim testTegmental areaMale ratsMood disordersVTA infusionsFST behaviorRatsPhysostigmine
2019
Cholinergic Receptor Blockade in the VTA Attenuates Cue-Induced Cocaine-Seeking and Reverses the Anxiogenic Effects of Forced Abstinence
Nunes EJ, Bitner L, Hughley SM, Small KM, Walton SN, Rupprecht LE, Addy NA. Cholinergic Receptor Blockade in the VTA Attenuates Cue-Induced Cocaine-Seeking and Reverses the Anxiogenic Effects of Forced Abstinence. Neuroscience 2019, 413: 252-263. PMID: 31271832, PMCID: PMC6661179, DOI: 10.1016/j.neuroscience.2019.06.028.Peer-Reviewed Original ResearchConceptsCue-induced cocaineAnxiety-related behaviorDrug relapseOpen arm timeCholinergic receptor blockadeCocaine abstinenceVentral tegmental areaSubstance abuseSelf-administer intravenous cocaineMuscarinic receptor antagonist scopolaminePeriod of abstinenceAnxiogenic effectsReceptor blockadeCholinergic mechanismsCocaine-naïve ratsNicotinic acetylcholine receptor antagonist mecamylamineMale Sprague-Dawley ratsDrug abstinenceSprague-Dawley ratsDose-dependent increaseCocaine-naive ratsAntagonist scopolamineEPM behaviorSaline ratsAbstinence
2018
The L-type calcium channel blocker, isradipine, attenuates cue-induced cocaine-seeking by enhancing dopaminergic activity in the ventral tegmental area to nucleus accumbens pathway
Addy NA, Nunes EJ, Hughley SM, Small KM, Baracz SJ, Haight JL, Rajadhyaksha AM. The L-type calcium channel blocker, isradipine, attenuates cue-induced cocaine-seeking by enhancing dopaminergic activity in the ventral tegmental area to nucleus accumbens pathway. Neuropsychopharmacology 2018, 43: 2361-2372. PMID: 29773910, PMCID: PMC6180103, DOI: 10.1038/s41386-018-0080-2.Peer-Reviewed Original ResearchConceptsL-type calcium channelsCocaine relapseLever depressionVentral tegmental areaCocaine self-administration trainingOriginal training contextLight cue presentationSelf-administration trainingSubjective effectsCocaine-seeking behaviorTegmental areaBehavioral effectsCocaine's subjective effectsL-type calcium channel blockerCue presentationMale Sprague-Dawley ratsTraining contextCocaine cuesNucleus accumbens coreNatural rewardsDA receptor activationCalcium channel blockersSprague-Dawley ratsPhasic dopaminePotential therapeutic target
2017
Evaluating oral flavorant effects on nicotine self-administration behavior and phasic dopamine signaling
Wickham RJ, Nunes EJ, Hughley S, Silva P, Walton SN, Park J, Addy NA. Evaluating oral flavorant effects on nicotine self-administration behavior and phasic dopamine signaling. Neuropharmacology 2017, 128: 33-42. PMID: 28943284, PMCID: PMC5714675, DOI: 10.1016/j.neuropharm.2017.09.029.Peer-Reviewed Original ResearchConceptsSelf-administration behaviorNucleus accumbensNicotine self-administration behaviorIntravenous self-administration paradigmFast-scan cyclic voltammetryPublic health policy decisionsPhasic DA signalingTobacco product useProgressive ratio taskSelf-administration paradigmTwo-bottle choice testPhasic DA releaseHealth policy decisionsTobacco product regulationOral mentholOral nicotineOral sucroseNicotine administrationPreclinical findingsDA releaseMale ratsOral administrationSmoking behaviorNicotine aversionMenthol effects
2010
Sensitization of Rapid Dopamine Signaling in the Nucleus Accumbens Core and Shell After Repeated Cocaine in Rats
Addy N, Daberkow D, Ford J, Garris P, Wightman R. Sensitization of Rapid Dopamine Signaling in the Nucleus Accumbens Core and Shell After Repeated Cocaine in Rats. Journal Of Neurophysiology 2010, 104: 922-931. PMID: 20554845, PMCID: PMC2934942, DOI: 10.1152/jn.00413.2010.Peer-Reviewed Original ResearchConceptsAcute cocaine challengeCocaine challengeNucleus accumbens coreWithdrawal periodDopamine sensitizationShort withdrawal periodDopamine releaseDopamine neurotransmissionFast-scan cyclic voltammetryAccumbens coreMale Sprague-Dawley ratsSubsequent cocaine challengeSprague-Dawley ratsLonger withdrawal periodsCocaine-treated subjectsPre-exposed subjectsRepeated CocaineDaily injectionsAcute cocaineDopaminergic responseCocaine exposureDopamine overflowNAc coreDopamine uptakeUptake inhibition
2002
Hippocampal α7 and α4β2 nicotinic receptors and working memory
Levin E, Bradley A, Addy N, Sigurani N. Hippocampal α7 and α4β2 nicotinic receptors and working memory. Neuroscience 2002, 109: 757-765. PMID: 11927157, DOI: 10.1016/s0306-4522(01)00538-3.Peer-Reviewed Original ResearchConceptsDH beta EAlpha 7 nicotinic receptorAlpha 4 beta 2Ventral hippocampusNicotinic receptorsLow dosesBeta EReference memory errorsCounter-balanced orderRadial mazeHigh dosesAdult female Sprague-Dawley ratsFemale Sprague-Dawley ratsNicotine-induced memory improvementAcute intrahippocampal infusionsVentral hippocampal infusionsBeta 2Nicotinic receptor blockadeNicotinic receptor agonistsSprague-Dawley ratsΑ4β2 nicotinic receptorsSignificant increaseChronic guide cannulaeHippocampal α7Nicotinic involvement
2001
Persistent behavioral consequences of neonatal chlorpyrifos exposure in rats
Levin E, Addy N, Nakajima A, Christopher N, Seidler F, Slotkin T. Persistent behavioral consequences of neonatal chlorpyrifos exposure in rats. Brain Research 2001, 130: 83-89. PMID: 11557096, DOI: 10.1016/s0165-3806(01)00215-2.Peer-Reviewed Original ResearchConceptsFigure-8 apparatusPostnatal CPF exposureCPF exposureEarly neonatal exposureMuscarinic cholinergic controlNeonatal chlorpyrifos exposurePersistent behavioral effectsRadial mazeT-mazeNicotinic cholinergic antagonistBrain cell developmentReference memory impairmentRadial arm mazeReference memory errorsPNDs 11Neonatal exposurePND 1Muscarinic antagonistNeonatal periodCholinergic antagonistsCholinergic controlNeurochemical changesRate of habituationCPF groupLong-term effects