2023
Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages
Oliveira G, Vogels C, Zolfaghari A, Saraf S, Klitting R, Weger-Lucarelli J, P Leon K, Ontiveros C, Agarwal R, Tsetsarkin K, Harris E, Ebel G, Wohl S, Grubaugh N, Andersen K. Genomic and phenotypic analyses suggest moderate fitness differences among Zika virus lineages. PLOS Neglected Tropical Diseases 2023, 17: e0011055. PMID: 36753510, PMCID: PMC9907835, DOI: 10.1371/journal.pntd.0011055.Peer-Reviewed Original ResearchConceptsHuman primary cellsFitness differencesVirus lineagesRapid molecular evolutionPrimary cellsShort generation timeAmino acid sitesFitness changesHigh mutation ratePhenotypic evolutionMolecular evolutionPositive selectionMutation rateLineagesPhenotypic analysisPhenotypic changesRNA virusesGeneration timeRecombinant virusesAedes aegypti mosquitoesReplicative fitnessFitnessAegypti mosquitoesMosquitoesZika virus
2021
Asynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic
Petrone ME, Earnest R, Lourenço J, Kraemer MUG, Paulino-Ramirez R, Grubaugh ND, Tapia L. Asynchronicity of endemic and emerging mosquito-borne disease outbreaks in the Dominican Republic. Nature Communications 2021, 12: 151. PMID: 33420058, PMCID: PMC7794562, DOI: 10.1038/s41467-020-20391-x.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAedesAnimalsChikungunya FeverChikungunya virusChildChild, PreschoolCommunicable Diseases, EmergingDengueDengue VirusDisease OutbreaksDominican RepublicEndemic DiseasesEpidemiological MonitoringFemaleHumansInfantInfant, NewbornMaleMosquito ControlMosquito VectorsSpatio-Temporal AnalysisYoung AdultZika VirusZika Virus InfectionConceptsDengue outbreakDisease outbreaksDengue fever casesOutbreak of chikungunyaMosquito-Borne VirusesFever casesFuture health crisesMosquito-borne disease outbreaksFuture outbreaksVirus transmissionAedes mosquitoesSustainable disease control measuresHealth crisisVirusDisease control measuresOutbreakChikungunyaConstant surveillanceZikaControl measuresDominican Republic
2020
Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors
Lemos D, Stuart JB, Louie W, Singapuri A, Ramírez AL, Watanabe J, Usachenko J, Keesler RI, Martin CS, Li T, Martyn C, Oliveira G, Saraf S, Grubaugh ND, Andersen KG, Thissen J, Allen J, Borucki M, Tsetsarkin KA, Pletnev AG, Chiu CY, Van Rompay KKA, Coffey LL. Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors. Journal Of Virology 2020, 94: 10.1128/jvi.01605-20. PMID: 32999034, PMCID: PMC7925200, DOI: 10.1128/jvi.01605-20.Peer-Reviewed Original ResearchConceptsCongenital Zika syndromeZika virusFetal infectionFetal deathZika syndromePregnant micePregnant wild-type miceRhesus macaque fetusesPregnant rhesus macaquesZika virus infectionWild-type miceNonpregnant hostsPlacental infectionLow viremiaMajority of animalsPregnant womenPregnant macaquesSevere outcomesMacaque fetusesVirus infectionFetusesInfectionZIKV polyproteinMouse fetusesRhesus macaques
2019
Travel Surveillance and Genomics Uncover a Hidden Zika Outbreak during the Waning Epidemic
Grubaugh ND, Saraf S, Gangavarapu K, Watts A, Tan AL, Oidtman RJ, Ladner JT, Oliveira G, Matteson NL, Kraemer MUG, Vogels CBF, Hentoff A, Bhatia D, Stanek D, Scott B, Landis V, Stryker I, Cone MR, Kopp EW, Cannons AC, Heberlein-Larson L, White S, Gillis LD, Ricciardi MJ, Kwal J, Lichtenberger PK, Magnani DM, Watkins DI, Palacios G, Hamer DH, Network G, Gardner LM, Perkins TA, Baele G, Khan K, Morrison A, Isern S, Michael SF, Andersen KG. Travel Surveillance and Genomics Uncover a Hidden Zika Outbreak during the Waning Epidemic. Cell 2019, 178: 1057-1071.e11. PMID: 31442400, PMCID: PMC6716374, DOI: 10.1016/j.cell.2019.07.018.Peer-Reviewed Original ResearchSmall RNA responses of Culex mosquitoes and cell lines during acute and persistent virus infection
Rückert C, Prasad AN, Garcia-Luna SM, Robison A, Grubaugh ND, Weger-Lucarelli J, Ebel GD. Small RNA responses of Culex mosquitoes and cell lines during acute and persistent virus infection. Insect Biochemistry And Molecular Biology 2019, 109: 13-23. PMID: 30959110, PMCID: PMC6516063, DOI: 10.1016/j.ibmb.2019.04.008.Peer-Reviewed Original ResearchConceptsCulex cellsSRNA responseViral DNA formsRNA interferenceMerida virusCulex mosquitoesSmall RNA responsesSmall RNA librariesCell linesWest Nile virusAntiviral RNA interferenceDNA formsHsu cellsVirus-derived DNANt siRNAsPiRNA responseRNA pathwaysSRNA readsRNA librariesWimpy testisEfficient antiviral responsePiRNAsAntiviral responseImportant virusesAedes sppArbovirus coinfection and co-transmission: A neglected public health concern?
Vogels CBF, Rückert C, Cavany SM, Perkins TA, Ebel GD, Grubaugh ND. Arbovirus coinfection and co-transmission: A neglected public health concern? PLOS Biology 2019, 17: e3000130. PMID: 30668574, PMCID: PMC6358106, DOI: 10.1371/journal.pbio.3000130.Peer-Reviewed Original ResearchConceptsImpact of coinfectionPublic health concernPublic healthEpidemiological synergyVirus coinfectionAedes aegypti mosquitoesClinical diseaseSuch coinfectionsZika virusCoinfectionOutbreaks of virusesHealth concernAegypti mosquitoesMultiple virusesTransmission dynamicsVirusHealthHumansMosquitoesDiseaseDengue
2017
Genomic epidemiology reveals multiple introductions of Zika virus into the United States
Grubaugh ND, Ladner JT, Kraemer MUG, Dudas G, Tan AL, Gangavarapu K, Wiley MR, White S, Thézé J, Magnani DM, Prieto K, Reyes D, Bingham AM, Paul LM, Robles-Sikisaka R, Oliveira G, Pronty D, Barcellona CM, Metsky HC, Baniecki ML, Barnes KG, Chak B, Freije CA, Gladden-Young A, Gnirke A, Luo C, MacInnis B, Matranga CB, Park DJ, Qu J, Schaffner SF, Tomkins-Tinch C, West KL, Winnicki SM, Wohl S, Yozwiak NL, Quick J, Fauver JR, Khan K, Brent SE, Reiner RC, Lichtenberger PN, Ricciardi MJ, Bailey VK, Watkins DI, Cone MR, Kopp EW, Hogan KN, Cannons AC, Jean R, Monaghan AJ, Garry RF, Loman NJ, Faria NR, Porcelli MC, Vasquez C, Nagle ER, Cummings DAT, Stanek D, Rambaut A, Sanchez-Lockhart M, Sabeti PC, Gillis LD, Michael SF, Bedford T, Pybus OG, Isern S, Palacios G, Andersen KG. Genomic epidemiology reveals multiple introductions of Zika virus into the United States. Nature 2017, 546: 401-405. PMID: 28538723, PMCID: PMC5536180, DOI: 10.1038/nature22400.Peer-Reviewed Original Research
2016
Genetic Drift during Systemic Arbovirus Infection of Mosquito Vectors Leads to Decreased Relative Fitness during Host Switching
Grubaugh ND, Weger-Lucarelli J, Murrieta RA, Fauver JR, Garcia-Luna SM, Prasad AN, Black WC, Ebel GD. Genetic Drift during Systemic Arbovirus Infection of Mosquito Vectors Leads to Decreased Relative Fitness during Host Switching. Cell Host & Microbe 2016, 19: 481-492. PMID: 27049584, PMCID: PMC4833525, DOI: 10.1016/j.chom.2016.03.002.Peer-Reviewed Original ResearchConceptsRelative fitnessMosquito speciesLower relative fitnessWest Nile virusComplex virus populationsSignificant fitness costMosquito-borne RNA virusesHost switchingGenetic driftGenetic diversityAdaptive potentialPopulation expansionFitness costsAvian cellsDeleterious mutationsMutational diversityRNA virusesBridge vectorsViral populationsVirus populationsMosquito vectorsFitnessSpeciesDiversityNile virus