2021
Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay
Beddingfield B, Hartnett J, Wilson R, Kulakosky P, Andersen K, Robles-Sikisaka R, Grubaugh N, Aybar A, Nunez M, Fermin C, Garry R. Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay. Viruses 2021, 13: 1771. PMID: 34578352, PMCID: PMC8473068, DOI: 10.3390/v13091771.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ViralAntigens, ViralCross ReactionsDengueDengue VirusEnzyme-Linked Immunosorbent AssayEpitopesFemaleFlavivirusHumansImmunoassayImmunologic TestsModels, MolecularMutagenesis, Site-DirectedPregnancyViral Nonstructural ProteinsWest Nile virusYellow fever virusZika VirusZika Virus InfectionConceptsZIKV nonstructural protein 1Nonstructural protein 1Antigen capture ELISADengue virusZika virusMild self-limiting illnessSelf-limiting illnessNS1 proteinMajor birth defectsSevere neurological diseaseYellow fever virusAntigen capture immunoassaySt. Louis encephalitis virusPolyclonal antibodiesLouis encephalitis virusWest Nile virusSerious outcomesAffinity-purified polyclonal antibodiesRelated flavivirusesNeurological diseasesRefinement of approachesEncephalitis virusWidespread flavivirusFlavivirusesFever virus
2020
Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors
Lemos D, Stuart JB, Louie W, Singapuri A, Ramírez AL, Watanabe J, Usachenko J, Keesler RI, Martin CS, Li T, Martyn C, Oliveira G, Saraf S, Grubaugh ND, Andersen KG, Thissen J, Allen J, Borucki M, Tsetsarkin KA, Pletnev AG, Chiu CY, Van Rompay KKA, Coffey LL. Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors. Journal Of Virology 2020, 94: 10.1128/jvi.01605-20. PMID: 32999034, PMCID: PMC7925200, DOI: 10.1128/jvi.01605-20.Peer-Reviewed Original ResearchConceptsCongenital Zika syndromeZika virusFetal infectionFetal deathZika syndromePregnant micePregnant wild-type miceRhesus macaque fetusesPregnant rhesus macaquesZika virus infectionWild-type miceNonpregnant hostsPlacental infectionLow viremiaMajority of animalsPregnant womenPregnant macaquesSevere outcomesMacaque fetusesVirus infectionFetusesInfectionZIKV polyproteinMouse fetusesRhesus macaques
2016
Isolation of a Novel Insect-Specific Flavivirus from Culiseta melanura in the Northeastern United States
Misencik MJ, Grubaugh ND, Andreadis TG, Ebel GD, Armstrong PM. Isolation of a Novel Insect-Specific Flavivirus from Culiseta melanura in the Northeastern United States. Vector-Borne And Zoonotic Diseases 2016, 16: 181-190. PMID: 26807512, PMCID: PMC4779966, DOI: 10.1089/vbz.2015.1889.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCulexCulicidaeFemaleFlavivirusFlavivirus InfectionsGenetic VariationInsect VectorsLarvaMaleNew EnglandPhylogenySeasonsSequence Analysis, DNAViral Nonstructural ProteinsConceptsInsect-specific flavivirusesOpen reading framePhylogenetic analysisDistinct evolutionary lineagesAmino acid sequence identityObvious geographic structureLimited genetic variationNovel insect-specific flavivirusEvolutionary lineagesHost associationsGeographic structureComplete genomeGenetic variationField-collected mosquitoesReading frameMelanura larvaeNucleotide sequenceSequence identityLarval stagesSampled populationsNortheastern United StatesGenus FlavivirusCalbertado virusNS5 geneViral sequences