Featured Publications
Mechanical stretch scales centriole number to apical area via Piezo1 in multiciliated cells
Kulkarni S, Marquez J, Date P, Ventrella R, Mitchell B, Khokha M. Mechanical stretch scales centriole number to apical area via Piezo1 in multiciliated cells. ELife 2021, 10: e66076. PMID: 34184636, PMCID: PMC8270640, DOI: 10.7554/elife.66076.Peer-Reviewed Original ResearchConceptsCentriole numberMulticiliated cellsCentriole amplificationHundreds of centriolesOptimal cell functionTension-dependent mannerMechanosensitive ion channelsEmbryonic elongationOrganelle numberCell biologyNumber controlIon channelsCentriolesMost cellsPiezo1Multiple ciliaCell functionMCC functionMechanical forcesApical areaCiliaCellsPotential roleAmplificationFundamental questionsWDR5 Stabilizes Actin Architecture to Promote Multiciliated Cell Formation
Kulkarni SS, Griffin JN, Date PP, Liem KF, Khokha MK. WDR5 Stabilizes Actin Architecture to Promote Multiciliated Cell Formation. Developmental Cell 2018, 46: 595-610.e3. PMID: 30205038, PMCID: PMC6177229, DOI: 10.1016/j.devcel.2018.08.009.Peer-Reviewed Original ResearchConceptsMulticiliated cellsF-actinBasal bodiesHundreds of ciliaApical actin networkApical F-actinApical actinChromatin modificationsChromatin modifiersTissue morphogenesisActin cytoskeletonActin architectureActin networkWDR5Intracellular organellesG-actinCell surfaceCell formationCellsCytoskeletonMorphogenesisOrganellesRegulatorActinBinds
2024
The Heterotaxy Gene CCDC11 Is Important for Cytokinesis via RhoA Regulation
Kulkarni S, Stephenson R, Amalraj S, Arrigo A, Betleja E, Moresco J, Yates J, Mahjoub M, Miller A, Khokha M. The Heterotaxy Gene CCDC11 Is Important for Cytokinesis via RhoA Regulation. Cytoskeleton 2024 PMID: 39479942, DOI: 10.1002/cm.21952.Peer-Reviewed Original ResearchRhoA regulationCytokinetic contractile ringLeft-right organizerLR patterningRhoA protein levelsContractile ringGTPase RhoADepletion phenotypeMonociliated cellsCCDC11Loss of functionCytokinesisRhoAPatient phenotypesPhenotypeDisease pathogenesisProtein levelsMultiple ciliaCellsPotential mechanismsRegulationGTPaseCFAP53MulticiliationMutations